Roseburia sp. CAG:197

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5YU86|R5YU86_9FIRM Riboflavin biosynthesis protein OS=Roseburia sp. CAG:197 OX=1262943 GN=BN528_01788 PE=3 SV=1
MM1 pKa = 7.83EE2 pKa = 6.29DD3 pKa = 3.92LFEE6 pKa = 4.3KK7 pKa = 10.92LKK9 pKa = 10.99DD10 pKa = 3.61RR11 pKa = 11.84EE12 pKa = 4.31SFDD15 pKa = 4.8AYY17 pKa = 9.49WSEE20 pKa = 4.19NYY22 pKa = 10.07VPITYY27 pKa = 10.6ADD29 pKa = 3.39VKK31 pKa = 10.68DD32 pKa = 3.96AYY34 pKa = 10.28EE35 pKa = 4.63DD36 pKa = 3.74FVKK39 pKa = 10.95ASDD42 pKa = 3.27KK43 pKa = 11.09HH44 pKa = 7.99IFVSDD49 pKa = 3.5YY50 pKa = 11.51GEE52 pKa = 4.21SGNINRR58 pKa = 11.84DD59 pKa = 3.13DD60 pKa = 4.35FMDD63 pKa = 4.15NLSQTAQFTFQDD75 pKa = 3.65SLTEE79 pKa = 3.96AFYY82 pKa = 11.27DD83 pKa = 3.98KK84 pKa = 11.43NPDD87 pKa = 3.31LYY89 pKa = 9.27EE90 pKa = 3.88TAFAIYY96 pKa = 10.28EE97 pKa = 4.04EE98 pKa = 4.39AQMNGGNDD106 pKa = 3.31EE107 pKa = 4.73NIAATFHH114 pKa = 6.5EE115 pKa = 5.35EE116 pKa = 3.8YY117 pKa = 10.7NRR119 pKa = 11.84LYY121 pKa = 11.0KK122 pKa = 10.62EE123 pKa = 4.45FLLAMYY129 pKa = 10.57DD130 pKa = 3.49AMFF133 pKa = 4.53

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5YMC7|R5YMC7_9FIRM Uncharacterized protein OS=Roseburia sp. CAG:197 OX=1262943 GN=BN528_00322 PE=4 SV=1
MM1 pKa = 7.99AYY3 pKa = 10.52NKK5 pKa = 8.97TAEE8 pKa = 4.31GSSMKK13 pKa = 9.8RR14 pKa = 11.84RR15 pKa = 11.84PMRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.25KK22 pKa = 9.78VCVFCGKK29 pKa = 10.59DD30 pKa = 3.18NVIDD34 pKa = 3.9YY35 pKa = 11.17KK36 pKa = 10.78DD37 pKa = 3.45TNKK40 pKa = 10.31LKK42 pKa = 10.49RR43 pKa = 11.84YY44 pKa = 8.14ISEE47 pKa = 4.0RR48 pKa = 11.84GKK50 pKa = 10.09ILPRR54 pKa = 11.84RR55 pKa = 11.84ITGNCAKK62 pKa = 9.81HH63 pKa = 5.44QRR65 pKa = 11.84ALTVAIKK72 pKa = 10.27RR73 pKa = 11.84ARR75 pKa = 11.84HH76 pKa = 5.31LAMMPYY82 pKa = 9.83VCEE85 pKa = 3.96

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2420

0

2420

741697

30

1517

306.5

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.453 ± 0.055

1.472 ± 0.019

5.941 ± 0.044

7.381 ± 0.06

3.97 ± 0.034

6.709 ± 0.042

1.781 ± 0.025

7.483 ± 0.05

7.313 ± 0.047

8.55 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.216 ± 0.025

4.687 ± 0.037

2.979 ± 0.029

3.511 ± 0.032

3.983 ± 0.038

5.724 ± 0.041

5.583 ± 0.046

7.096 ± 0.043

0.842 ± 0.016

4.321 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski