Ferrimicrobium acidiphilum DSM 19497

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Acidimicrobiaceae; Ferrimicrobium; Ferrimicrobium acidiphilum

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8FRF9|A0A0D8FRF9_9ACTN Uncharacterized protein OS=Ferrimicrobium acidiphilum DSM 19497 OX=1121877 GN=FEAC_25480 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 3.51FRR5 pKa = 11.84VTDD8 pKa = 3.38VAVEE12 pKa = 4.31VVLEE16 pKa = 3.9ALADD20 pKa = 3.92EE21 pKa = 5.04GQTDD25 pKa = 5.07ALALWIEE32 pKa = 4.22VAGNDD37 pKa = 4.35GASFMYY43 pKa = 10.1DD44 pKa = 2.88VYY46 pKa = 10.91FQEE49 pKa = 5.35LAQADD54 pKa = 4.29PDD56 pKa = 4.19DD57 pKa = 4.0LHH59 pKa = 8.71LHH61 pKa = 6.09FGEE64 pKa = 4.49LNVVIPKK71 pKa = 9.67TSIAQLNGATLEE83 pKa = 4.2VGGDD87 pKa = 3.65GEE89 pKa = 5.11LAITNPNTPTPEE101 pKa = 4.68ASDD104 pKa = 4.54LPDD107 pKa = 4.89LGPEE111 pKa = 3.85ALANDD116 pKa = 3.95LALRR120 pKa = 11.84VNAILEE126 pKa = 4.35EE127 pKa = 4.18QVNPAIAAHH136 pKa = 6.43GGYY139 pKa = 10.41AEE141 pKa = 4.34LVGVVDD147 pKa = 4.04ARR149 pKa = 11.84AYY151 pKa = 10.26VLMGGGCQGCGLAAMTLSQGIATAIQEE178 pKa = 4.47AVPEE182 pKa = 4.1IVDD185 pKa = 3.36IVDD188 pKa = 3.8VTNHH192 pKa = 6.18AAGNNPYY199 pKa = 9.62YY200 pKa = 10.65QPAKK204 pKa = 10.23KK205 pKa = 10.29

Molecular weight: 21.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8FVA5|A0A0D8FVA5_9ACTN Uncharacterized protein OS=Ferrimicrobium acidiphilum DSM 19497 OX=1121877 GN=FEAC_20590 PE=4 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84GVGLNTHH10 pKa = 5.67TLGFKK15 pKa = 10.64GSVAFTSAAIVLVRR29 pKa = 11.84SRR31 pKa = 11.84ASPSNRR37 pKa = 11.84AVRR40 pKa = 11.84QAHH43 pKa = 5.77GGGPRR48 pKa = 11.84RR49 pKa = 11.84QAMLLGNRR57 pKa = 11.84RR58 pKa = 11.84SS59 pKa = 3.32

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2974

0

2974

857107

29

1825

288.2

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.484 ± 0.049

0.85 ± 0.014

5.289 ± 0.043

5.834 ± 0.048

3.309 ± 0.024

8.18 ± 0.044

2.265 ± 0.021

5.364 ± 0.035

2.664 ± 0.032

10.878 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.018

2.666 ± 0.028

4.935 ± 0.029

3.272 ± 0.026

6.992 ± 0.047

7.122 ± 0.05

5.793 ± 0.052

8.354 ± 0.046

1.294 ± 0.019

2.387 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski