Anaeromassilibacillus sp. An200

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaeromassilibacillus; unclassified Anaeromassilibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3479 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4HJH9|A0A1Y4HJH9_9FIRM ABC transmembrane type-1 domain-containing protein OS=Anaeromassilibacillus sp. An200 OX=1965587 GN=B5F35_13655 PE=3 SV=1
MM1 pKa = 7.88EE2 pKa = 6.17DD3 pKa = 3.37FVSCVTLADD12 pKa = 3.82GSRR15 pKa = 11.84IVLDD19 pKa = 4.83DD20 pKa = 3.99EE21 pKa = 6.03DD22 pKa = 4.7NVLLSLLSHH31 pKa = 6.59NGVEE35 pKa = 4.26DD36 pKa = 3.7TLQFSHH42 pKa = 7.43LSGNYY47 pKa = 9.96GGGSLLLSPSQKK59 pKa = 10.78YY60 pKa = 10.79LIFSYY65 pKa = 10.72FSGEE69 pKa = 4.18SEE71 pKa = 4.03EE72 pKa = 5.21GFALLEE78 pKa = 4.03IANNRR83 pKa = 11.84LKK85 pKa = 10.94LLYY88 pKa = 10.61DD89 pKa = 3.19SDD91 pKa = 4.07YY92 pKa = 11.38LYY94 pKa = 11.46GEE96 pKa = 4.53DD97 pKa = 4.44ANYY100 pKa = 11.01SFTNNEE106 pKa = 3.98TIIIQTFRR114 pKa = 11.84TGAWYY119 pKa = 10.26QDD121 pKa = 3.5DD122 pKa = 4.29ASIDD126 pKa = 3.93EE127 pKa = 4.77NGDD130 pKa = 3.04MYY132 pKa = 11.73YY133 pKa = 10.69EE134 pKa = 4.18FGEE137 pKa = 4.36LNLLNLEE144 pKa = 4.34TYY146 pKa = 10.9DD147 pKa = 4.57LDD149 pKa = 3.42RR150 pKa = 11.84HH151 pKa = 6.15TILVYY156 pKa = 10.15PSADD160 pKa = 2.88WKK162 pKa = 11.23EE163 pKa = 4.11EE164 pKa = 3.88EE165 pKa = 4.14TDD167 pKa = 3.05AGTFLFSDD175 pKa = 3.93ITSGMLKK182 pKa = 9.44IEE184 pKa = 4.37MPWGSEE190 pKa = 3.91SFPFPLQEE198 pKa = 3.88TLIVKK203 pKa = 9.02FNKK206 pKa = 10.02

Molecular weight: 23.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4HMA5|A0A1Y4HMA5_9FIRM Uncharacterized protein OS=Anaeromassilibacillus sp. An200 OX=1965587 GN=B5F35_16665 PE=4 SV=1
MM1 pKa = 7.27RR2 pKa = 11.84QWEE5 pKa = 4.44KK6 pKa = 10.36CGKK9 pKa = 4.56WTRR12 pKa = 11.84AAIIGSLCGLLNGLFGAGGGMAAVPLLRR40 pKa = 11.84AGGLEE45 pKa = 3.93QKK47 pKa = 9.93KK48 pKa = 9.57AHH50 pKa = 5.81ATSLAVILPLAAVSAVSYY68 pKa = 10.87LMAEE72 pKa = 4.09AVSFSQAAPYY82 pKa = 10.42LPGGFVGACAGALLMNRR99 pKa = 11.84IPSGMLRR106 pKa = 11.84RR107 pKa = 11.84VFGGLLIFAGVRR119 pKa = 11.84LLLRR123 pKa = 4.29

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3479

0

3479

1072320

23

2544

308.2

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.909 ± 0.049

1.704 ± 0.02

5.345 ± 0.036

7.398 ± 0.04

4.083 ± 0.032

7.417 ± 0.041

1.875 ± 0.019

5.65 ± 0.044

4.881 ± 0.035

9.896 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.02

3.446 ± 0.029

4.267 ± 0.027

3.689 ± 0.028

5.886 ± 0.045

6.123 ± 0.035

5.344 ± 0.035

6.752 ± 0.033

1.103 ± 0.019

3.536 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski