Endomicrobium proavitum

Taxonomy: cellular organisms; Bacteria; Elusimicrobia; Endomicrobia; Endomicrobiales; Endomicrobiaceae; Endomicrobium

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3WJA7|A0A0G3WJA7_9BACT Uncharacterized protein OS=Endomicrobium proavitum OX=1408281 GN=Epro_1044 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.49KK3 pKa = 10.47SMHH6 pKa = 6.34SKK8 pKa = 9.22WNPFICMLLSFCMTVYY24 pKa = 10.82AFSPVYY30 pKa = 10.18AVNVTQSSDD39 pKa = 3.33FASNWNSAVSPIVVVGAPIDD59 pKa = 3.78SNGNYY64 pKa = 10.5GIILSNYY71 pKa = 8.98LNAIGTNMSLVGYY84 pKa = 9.42SGSPDD89 pKa = 3.25GEE91 pKa = 4.1MLVLGQYY98 pKa = 11.06ALAFAPGWSNDD109 pKa = 2.72INVNISSLSISGATNGAVSFQGNGTGAHH137 pKa = 6.17NLSVSDD143 pKa = 3.15IYY145 pKa = 10.97FYY147 pKa = 11.65NNQFASGNGGAINVGTQIQSVANNKK172 pKa = 9.82FILTDD177 pKa = 3.32NDD179 pKa = 4.15FISNKK184 pKa = 8.8AQNGGAVYY192 pKa = 10.3VSAVNAYY199 pKa = 9.99LSSLLQPVFSVIDD212 pKa = 3.43TTNNNGHH219 pKa = 5.91YY220 pKa = 10.05FAFNQATNGNGGALYY235 pKa = 9.7FASQNAQSTVVNQFTAQGYY254 pKa = 9.61SYY256 pKa = 9.7YY257 pKa = 11.23ANYY260 pKa = 10.89ASGLGGAIYY269 pKa = 10.72NGVSNGNSPAVNEE282 pKa = 4.26FNISSGVFSKK292 pKa = 10.92NQAGSGGAVATTVNGGNTAVNVVINGEE319 pKa = 4.34FYY321 pKa = 11.45SNAATNGGGAVYY333 pKa = 10.66NFVGDD338 pKa = 3.34QSSPYY343 pKa = 9.89GGNINFNVNGIFDD356 pKa = 3.84GNKK359 pKa = 9.45AVNGGAIYY367 pKa = 11.04NNIQPGQATIVTNAGGSFTNNFASGNGGAIYY398 pKa = 8.51NTAGLNTAILNIADD412 pKa = 3.7GTLFWGNGASYY423 pKa = 10.9GGAIFNSGSGVINLNTVSGNAITFGNNYY451 pKa = 9.03AANALNGADD460 pKa = 4.05IYY462 pKa = 11.11QDD464 pKa = 3.19SANAVINITGSGTVNINDD482 pKa = 3.63GFGGIGTINQQTGTTLNLNAPGINSGFTGTFNQAVGSILNASGVMFGGQNNIGGTANINSSQNSFYY548 pKa = 11.08FNANMLSGATLNFTSGSNDD567 pKa = 2.91RR568 pKa = 11.84VSIGVASSVSSPGIRR583 pKa = 11.84FQGTGASVGFNSSVSSGSAYY603 pKa = 11.34NLMQDD608 pKa = 3.79ISNGQSNSVNFNNSNVTFGSTQFTGATAYY637 pKa = 10.28GFYY640 pKa = 10.51NNSVINLADD649 pKa = 3.39SSSNYY654 pKa = 5.97QTYY657 pKa = 10.7NFSTLTTDD665 pKa = 3.55GSGVMNLKK673 pKa = 10.05IGNDD677 pKa = 3.49QNALLSDD684 pKa = 4.01AVNVQNGGGNIGLGKK699 pKa = 10.03IYY701 pKa = 10.47INDD704 pKa = 3.65EE705 pKa = 3.88EE706 pKa = 4.51GLITGYY712 pKa = 9.34MRR714 pKa = 11.84IIYY717 pKa = 8.65GGPLKK722 pKa = 10.3FVNGLTQYY730 pKa = 10.8VATSTGTYY738 pKa = 9.74TITTANDD745 pKa = 3.44YY746 pKa = 10.23YY747 pKa = 11.43VQFNSIPQTPPSGGVVVSTAGAGGDD772 pKa = 3.47TSSTINNIDD781 pKa = 3.36GSTTTVNTNTDD792 pKa = 3.13GSASSTTNNVDD803 pKa = 3.22GSSTTVTTGTDD814 pKa = 3.29GSASVTNTGSGGTDD828 pKa = 3.12TSASTTNPDD837 pKa = 2.89GSTSTTVTNPDD848 pKa = 2.98GSTTTVTTNPDD859 pKa = 2.96GSTSTTNTNAGGTTTSQSTTNPDD882 pKa = 2.79GSTTTTITNPDD893 pKa = 3.51GSTVTTTTNPDD904 pKa = 2.66GSTSVTHH911 pKa = 6.69TDD913 pKa = 2.73STGQSTTVVTNPDD926 pKa = 2.98GSAVITNPDD935 pKa = 3.26SSTTPINPGDD945 pKa = 4.07STSVTTPDD953 pKa = 3.41GSTVDD958 pKa = 3.67VNAGGDD964 pKa = 3.68GTTTTNVTPSGGGNASATTNPDD986 pKa = 2.61GTTTSTTTDD995 pKa = 2.97ADD997 pKa = 3.73GNVINNTTGSDD1008 pKa = 3.72GTSTTTDD1015 pKa = 3.02TTAGGGTAGTTTNPDD1030 pKa = 2.87GTTTSTTTDD1039 pKa = 3.24SNGNTIDD1046 pKa = 4.12SSTSSDD1052 pKa = 3.51GNSTTTNTTPGGTTTITTNPGDD1074 pKa = 3.71NTTVVTDD1081 pKa = 3.43SGGNTTTVITNPDD1094 pKa = 3.39GGGSSASLPDD1104 pKa = 3.39GTTTSTTVDD1113 pKa = 3.57NGDD1116 pKa = 3.2STAQINTPNNQPSLVLVLNDD1136 pKa = 3.19VNAVSVAAFGAGISSSTPRR1155 pKa = 11.84SFQIGANEE1163 pKa = 4.25TYY1165 pKa = 11.2YY1166 pKa = 11.56NDD1168 pKa = 3.92ANLDD1172 pKa = 3.52AMAGGVFTVFGANPGTRR1189 pKa = 11.84TSILSGLNATDD1200 pKa = 3.95LTTQQSLFNIASDD1213 pKa = 4.01SITFTLEE1220 pKa = 3.77DD1221 pKa = 3.53LTITNAYY1228 pKa = 8.13AASGGSVLNMNAANSSAYY1246 pKa = 10.33LGNLLITGNSSGADD1260 pKa = 2.94GGAINVSNGSVYY1272 pKa = 9.21ITGVDD1277 pKa = 4.89FISNSAAGNGGAISNAGGTVVQSGSFIGNSAVNGGAIYY1315 pKa = 8.56NTALAAAPMSIYY1327 pKa = 10.66SATSNLLLSNNTAAALGGAIYY1348 pKa = 10.63NDD1350 pKa = 3.28GFMTIGSATGADD1362 pKa = 3.0IVFSSNTANGVANDD1376 pKa = 4.13IYY1378 pKa = 11.55NSGTLNFASTGGNILITGGIAGSAAGIINKK1408 pKa = 9.74NGLADD1413 pKa = 3.67VVLAATADD1421 pKa = 3.33NSQYY1425 pKa = 11.26LGAFNMTNGIVDD1437 pKa = 3.75ANGKK1441 pKa = 9.51FFGGQSTVQSGILNWNAGASKK1462 pKa = 10.62EE1463 pKa = 4.02EE1464 pKa = 4.17SAILAVTGGVINIYY1478 pKa = 10.99GNLILANANDD1488 pKa = 4.55VIGQYY1493 pKa = 11.63ANLNLFSGGYY1503 pKa = 8.8YY1504 pKa = 9.63EE1505 pKa = 4.28VAGGSVFIDD1514 pKa = 3.6SNDD1517 pKa = 3.43MLVAPDD1523 pKa = 4.2GSYY1526 pKa = 11.58GSAKK1530 pKa = 9.96MSDD1533 pKa = 2.97GHH1535 pKa = 7.17LVLNGQGLVVYY1546 pKa = 10.39SGAYY1550 pKa = 8.12NQTGGVLHH1558 pKa = 6.46VEE1560 pKa = 4.34NQAVTYY1566 pKa = 8.35TIYY1569 pKa = 10.86EE1570 pKa = 4.39GAQSNNGILGGDD1582 pKa = 3.33ISITNARR1589 pKa = 11.84VNVLWYY1595 pKa = 10.47NFNISSGTGSIIPTKK1610 pKa = 10.54GSLSMGDD1617 pKa = 3.02GALFYY1622 pKa = 10.54TVDD1625 pKa = 3.53NTLQTHH1631 pKa = 5.82TFAGDD1636 pKa = 3.42FALFSAVSAFNTAANFEE1653 pKa = 4.27VDD1655 pKa = 5.21LDD1657 pKa = 4.09AQAHH1661 pKa = 6.11KK1662 pKa = 11.0SDD1664 pKa = 3.13ILVFGGTAAPGTIVSQGNPAAGIADD1689 pKa = 3.73VVASSGVVNLSGINFINSPKK1709 pKa = 10.32DD1710 pKa = 3.04AVVPFRR1716 pKa = 11.84IMDD1719 pKa = 4.04APSFDD1724 pKa = 3.47AGIIFSATTKK1734 pKa = 10.28RR1735 pKa = 11.84YY1736 pKa = 6.08YY1737 pKa = 10.31TPIGIYY1743 pKa = 10.16DD1744 pKa = 4.4LYY1746 pKa = 11.62SFGEE1750 pKa = 4.26GNYY1753 pKa = 8.3EE1754 pKa = 4.7LRR1756 pKa = 11.84MDD1758 pKa = 4.02SLNPVTLRR1766 pKa = 11.84AEE1768 pKa = 4.18AAVLAMIDD1776 pKa = 3.57AQNVSNSVLFDD1787 pKa = 3.41HH1788 pKa = 7.08VFFDD1792 pKa = 4.49SNQMLVNANKK1802 pKa = 10.26NEE1804 pKa = 3.68YY1805 pKa = 10.17RR1806 pKa = 11.84FLPRR1810 pKa = 11.84QFLKK1814 pKa = 10.66EE1815 pKa = 4.0NRR1817 pKa = 11.84EE1818 pKa = 3.2KK1819 pKa = 10.95DD1820 pKa = 3.58YY1821 pKa = 10.68WIKK1824 pKa = 9.99TYY1826 pKa = 10.6YY1827 pKa = 8.99EE1828 pKa = 4.15HH1829 pKa = 7.32EE1830 pKa = 4.43AFSVIDD1836 pKa = 3.69NTDD1839 pKa = 3.24LKK1841 pKa = 11.15NNLYY1845 pKa = 10.65GAILGLDD1852 pKa = 4.18FTASDD1857 pKa = 3.59IGEE1860 pKa = 4.19NSYY1863 pKa = 11.21FMPTVFAGYY1872 pKa = 8.33TGASQTLDD1880 pKa = 3.33AASMHH1885 pKa = 5.6QNAVKK1890 pKa = 10.48AGFMASAMVNDD1901 pKa = 4.75VYY1903 pKa = 10.88TVSALVYY1910 pKa = 9.65GGLYY1914 pKa = 8.47QNHH1917 pKa = 6.11MEE1919 pKa = 4.42VDD1921 pKa = 3.69GAEE1924 pKa = 4.35DD1925 pKa = 3.93NLNNWFAGVSVKK1937 pKa = 10.34NAYY1940 pKa = 10.36DD1941 pKa = 3.35MYY1943 pKa = 10.78IDD1945 pKa = 4.23NFVLQPFVTFSYY1957 pKa = 10.79NIYY1960 pKa = 10.59GSQKK1964 pKa = 8.15WNSSYY1969 pKa = 11.41GSIAMQTDD1977 pKa = 3.3NVDD1980 pKa = 3.56GFNASPGVNIIYY1992 pKa = 10.66GLDD1995 pKa = 2.83TWNFVIGEE2003 pKa = 4.24AYY2005 pKa = 10.34VYY2007 pKa = 11.16NLGNKK2012 pKa = 9.51ISGKK2016 pKa = 9.53IGNIEE2021 pKa = 4.91LPDD2024 pKa = 3.94LNNGIGYY2031 pKa = 8.38FEE2033 pKa = 4.22WTAGASKK2040 pKa = 10.7LFSKK2044 pKa = 10.34QLNVWLQAVYY2054 pKa = 8.63KK2055 pKa = 8.42TNRR2058 pKa = 11.84DD2059 pKa = 2.69ADD2061 pKa = 3.6IGVKK2065 pKa = 10.31AGAGWRR2071 pKa = 11.84FF2072 pKa = 3.12

Molecular weight: 211.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3WJQ1|A0A0G3WJQ1_9BACT Uncharacterized protein OS=Endomicrobium proavitum OX=1408281 GN=Epro_1134 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.31QPNKK9 pKa = 9.78DD10 pKa = 3.43RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.9KK14 pKa = 10.69KK15 pKa = 8.91IGFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.2GRR39 pKa = 11.84KK40 pKa = 7.48TISAA44 pKa = 3.98

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1336

0

1336

479164

27

8155

358.7

39.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.874 ± 0.106

0.922 ± 0.037

4.942 ± 0.064

5.789 ± 0.121

4.959 ± 0.062

7.407 ± 0.183

1.31 ± 0.034

8.017 ± 0.065

8.676 ± 0.187

8.321 ± 0.115

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.039

6.093 ± 0.176

2.988 ± 0.08

2.764 ± 0.044

3.191 ± 0.074

6.893 ± 0.109

5.4 ± 0.15

6.879 ± 0.049

0.782 ± 0.025

3.848 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski