Desulfotomaculum arcticum DSM 17038

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Desulfallaceae; Desulfallas; Desulfallas arcticus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5051 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1I2PJJ3|A0A1I2PJJ3_9FIRM ABC-2 type transport system permease protein OS=Desulfotomaculum arcticum DSM 17038 OX=1121424 GN=SAMN05660649_00907 PE=4 SV=1
MM1 pKa = 7.23YY2 pKa = 10.32IFVKK6 pKa = 10.48GYY8 pKa = 10.71NLAKK12 pKa = 10.38DD13 pKa = 3.51RR14 pKa = 11.84NGRR17 pKa = 11.84LINIIVRR24 pKa = 11.84TLRR27 pKa = 11.84KK28 pKa = 9.61YY29 pKa = 7.68IHH31 pKa = 7.01KK32 pKa = 10.61YY33 pKa = 10.45GILFLLLCLFSTMTACEE50 pKa = 4.24SKK52 pKa = 10.81DD53 pKa = 3.15ILGDD57 pKa = 3.76DD58 pKa = 4.11EE59 pKa = 5.84PEE61 pKa = 4.0VSQNATEE68 pKa = 4.68DD69 pKa = 3.47GTMGSSNDD77 pKa = 3.69GVNTEE82 pKa = 4.06ADD84 pKa = 3.26KK85 pKa = 11.38GAEE88 pKa = 3.83QSTAPNEE95 pKa = 4.22LEE97 pKa = 4.33DD98 pKa = 4.14TVSGQDD104 pKa = 3.96GEE106 pKa = 4.38EE107 pKa = 4.12TQMNEE112 pKa = 3.52ADD114 pKa = 3.65IDD116 pKa = 3.85RR117 pKa = 11.84EE118 pKa = 4.27AMMNEE123 pKa = 4.07YY124 pKa = 10.69KK125 pKa = 10.76SILKK129 pKa = 10.36GIYY132 pKa = 9.44DD133 pKa = 3.5NHH135 pKa = 6.7IFPDD139 pKa = 4.37GQACDD144 pKa = 3.13WDD146 pKa = 3.92GRR148 pKa = 11.84SDD150 pKa = 3.44VTQNQFAVYY159 pKa = 10.22DD160 pKa = 4.02IDD162 pKa = 5.61QDD164 pKa = 4.09TQDD167 pKa = 4.34EE168 pKa = 5.12LILAYY173 pKa = 9.41TNSSMAGMIEE183 pKa = 5.15LIYY186 pKa = 11.14DD187 pKa = 3.95FDD189 pKa = 5.41NDD191 pKa = 3.38TKK193 pKa = 10.56TANEE197 pKa = 4.12EE198 pKa = 3.85FRR200 pKa = 11.84EE201 pKa = 4.46FPTLTYY207 pKa = 10.19YY208 pKa = 11.24DD209 pKa = 3.58NGIIKK214 pKa = 10.32ADD216 pKa = 3.23WSHH219 pKa = 5.59NQGLAGDD226 pKa = 4.19FWPYY230 pKa = 10.32TLYY233 pKa = 10.77QYY235 pKa = 11.03DD236 pKa = 4.35KK237 pKa = 11.11KK238 pKa = 10.93SDD240 pKa = 3.6SYY242 pKa = 11.84VDD244 pKa = 3.4VGMVDD249 pKa = 2.56AWDD252 pKa = 4.23RR253 pKa = 11.84SLSATDD259 pKa = 3.7YY260 pKa = 11.4NGDD263 pKa = 3.85PFPAEE268 pKa = 4.22SDD270 pKa = 3.3TDD272 pKa = 3.76GDD274 pKa = 4.34GIVYY278 pKa = 10.27YY279 pKa = 10.31IMQDD283 pKa = 3.06GEE285 pKa = 4.6YY286 pKa = 10.61KK287 pKa = 10.23LDD289 pKa = 3.69HH290 pKa = 7.17PIDD293 pKa = 3.96GAEE296 pKa = 3.87YY297 pKa = 8.46EE298 pKa = 4.16QWRR301 pKa = 11.84DD302 pKa = 3.46FYY304 pKa = 10.92IGNAKK309 pKa = 9.28EE310 pKa = 3.97VNIPFINLTEE320 pKa = 4.02EE321 pKa = 4.45NIDD324 pKa = 3.97NIQQ327 pKa = 3.01

Molecular weight:
37.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1I2ZC58|A0A1I2ZC58_9FIRM Transposase DDE domain-containing protein (Fragment) OS=Desulfotomaculum arcticum DSM 17038 OX=1121424 GN=SAMN05660649_04876 PE=4 SV=1
MM1 pKa = 7.68PKK3 pKa = 9.97IKK5 pKa = 8.1THH7 pKa = 6.4RR8 pKa = 11.84GAAKK12 pKa = 10.0RR13 pKa = 11.84FKK15 pKa = 9.91KK16 pKa = 9.16TGSGKK21 pKa = 10.45FKK23 pKa = 10.02TSHH26 pKa = 6.54AFHH29 pKa = 6.38SHH31 pKa = 5.49ILGKK35 pKa = 8.81KK36 pKa = 5.69TPKK39 pKa = 9.96RR40 pKa = 11.84KK41 pKa = 9.57RR42 pKa = 11.84KK43 pKa = 9.1LRR45 pKa = 11.84KK46 pKa = 9.37ALIVHH51 pKa = 6.96PANAANLQRR60 pKa = 11.84LLPP63 pKa = 4.35

Molecular weight:
7.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5051

0

5051

1486842

39

3953

294.4

32.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.343 ± 0.037

1.232 ± 0.018

5.067 ± 0.034

6.55 ± 0.032

3.915 ± 0.026

7.754 ± 0.03

1.738 ± 0.017

7.329 ± 0.031

6.026 ± 0.028

10.057 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.018

4.374 ± 0.027

4.133 ± 0.02

3.511 ± 0.024

5.002 ± 0.032

5.466 ± 0.023

5.091 ± 0.032

7.347 ± 0.033

1.015 ± 0.014

3.331 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski