Paraburkholderia sp. BL23I1N1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R7ESB8|A0A3R7ESB8_9BURK LuxR family two component transcriptional regulator OS=Paraburkholderia sp. BL23I1N1 OX=1938802 GN=B0G76_1871 PE=4 SV=1
MM1 pKa = 7.03EE2 pKa = 4.97TLAQPASATPGVMPGLLEE20 pKa = 3.99FTPQAAAKK28 pKa = 8.83VASLIEE34 pKa = 4.23EE35 pKa = 4.21EE36 pKa = 4.73GNPNLKK42 pKa = 10.22LRR44 pKa = 11.84LYY46 pKa = 10.67VSGGGCSGFQYY57 pKa = 10.88GFAFDD62 pKa = 4.43DD63 pKa = 3.8QVVDD67 pKa = 5.61DD68 pKa = 5.56DD69 pKa = 4.42MQTVTDD75 pKa = 4.79GVTLLVDD82 pKa = 4.04SMSQQYY88 pKa = 10.93LFGARR93 pKa = 11.84VDD95 pKa = 4.01YY96 pKa = 11.17EE97 pKa = 4.54DD98 pKa = 4.21GLEE101 pKa = 3.97GSRR104 pKa = 11.84FVIHH108 pKa = 6.59NPNAQSNCGCGSSFSVV124 pKa = 3.54

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419Z068|A0A419Z068_9BURK RND family efflux transporter MFP subunit OS=Paraburkholderia sp. BL23I1N1 OX=1938802 GN=B0G76_7139 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7914

0

7914

2467661

30

2820

311.8

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.468 ± 0.039

0.978 ± 0.01

5.324 ± 0.018

5.113 ± 0.027

3.733 ± 0.018

8.106 ± 0.03

2.333 ± 0.013

4.767 ± 0.018

3.17 ± 0.021

10.139 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.012

2.92 ± 0.019

5.033 ± 0.019

3.67 ± 0.019

6.851 ± 0.03

5.886 ± 0.021

5.553 ± 0.026

7.721 ± 0.021

1.375 ± 0.01

2.459 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski