Defluviimonas denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Defluviimonas

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8S7F9|A0A2S8S7F9_9RHOB Regulatory LuxR family protein OS=Defluviimonas denitrificans OX=404881 GN=LX70_02325 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.94MKK4 pKa = 10.37SHH6 pKa = 6.7TLAAVLCSAALFAAPTVASADD27 pKa = 3.76SMSGMDD33 pKa = 4.18GMSGMGGMSGMSGMGGMSGMGGMSGMSGMGGMSGMSGMSGNGGMSWVCTQWTSTGMGGMSGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMAGMDD135 pKa = 3.73GMSGMGGMSGMGGMSGMGGMAGMDD159 pKa = 3.73GMSGMGGMAGMDD171 pKa = 3.73GMSGMGGMAGMDD183 pKa = 3.73GMSGMGGMAGMDD195 pKa = 3.73GMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMAGNASGWVCTQWTVSGPAMSGMSGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMGGMSGMDD307 pKa = 3.65GMSGMDD313 pKa = 3.38GMSGNN318 pKa = 4.11

Molecular weight: 29.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8S6C5|A0A2S8S6C5_9RHOB Uncharacterized protein OS=Defluviimonas denitrificans OX=404881 GN=LX70_02608 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44AGRR28 pKa = 11.84KK29 pKa = 8.46VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.53GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4024

0

4024

1248739

40

2640

310.3

33.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.25 ± 0.056

0.863 ± 0.011

5.917 ± 0.029

5.587 ± 0.037

3.666 ± 0.022

9.174 ± 0.04

1.997 ± 0.019

5.026 ± 0.029

3.067 ± 0.029

10.103 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.019

2.314 ± 0.02

5.285 ± 0.03

2.781 ± 0.019

7.11 ± 0.042

4.693 ± 0.023

5.47 ± 0.033

7.322 ± 0.029

1.451 ± 0.018

2.204 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski