Pseudonocardia hydrocarbonoxydans

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y3WMQ7|A0A4Y3WMQ7_9PSEU Uncharacterized protein OS=Pseudonocardia hydrocarbonoxydans OX=76726 GN=PHY01_23780 PE=4 SV=1
MM1 pKa = 7.39PTRR4 pKa = 11.84DD5 pKa = 3.77TAWPAGTPCWVDD17 pKa = 3.07LAVPDD22 pKa = 4.84LPAATAFYY30 pKa = 10.37GAVLGWEE37 pKa = 4.32FVDD40 pKa = 3.42TGEE43 pKa = 4.44EE44 pKa = 4.04FGHH47 pKa = 5.43YY48 pKa = 9.13TLAQVGGRR56 pKa = 11.84SAAAIAPVQPGQPSGSTLYY75 pKa = 10.69LASDD79 pKa = 4.47DD80 pKa = 3.88VDD82 pKa = 3.45ATAKK86 pKa = 10.39LVAEE90 pKa = 4.65HH91 pKa = 6.86GGTVAVEE98 pKa = 4.05PFDD101 pKa = 3.84VSGMGRR107 pKa = 11.84MAVALDD113 pKa = 3.42ASGAAFGIWQQAGMIGAEE131 pKa = 4.51VYY133 pKa = 10.58NEE135 pKa = 4.12PGSLIWEE142 pKa = 4.5DD143 pKa = 4.46ARR145 pKa = 11.84LTDD148 pKa = 4.08AAAGQAFYY156 pKa = 10.86AAVFGHH162 pKa = 6.24TFGAVPGAPDD172 pKa = 4.11DD173 pKa = 4.13YY174 pKa = 11.35VTFAVDD180 pKa = 3.61GEE182 pKa = 4.96VVGGMGGMMGAPEE195 pKa = 4.42GTPSHH200 pKa = 6.64WLPYY204 pKa = 10.68FMVSDD209 pKa = 3.79VDD211 pKa = 3.48AALAAVGSGGGTVLMEE227 pKa = 4.35ATDD230 pKa = 3.89TPFGRR235 pKa = 11.84MGIATDD241 pKa = 3.65PFGATFALHH250 pKa = 6.68GGMPAAA256 pKa = 5.23

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y3WJU5|A0A4Y3WJU5_9PSEU Uncharacterized protein OS=Pseudonocardia hydrocarbonoxydans OX=76726 GN=PHY01_15100 PE=4 SV=1
MM1 pKa = 7.23NRR3 pKa = 11.84LLGALRR9 pKa = 11.84GAANPGHH16 pKa = 7.11PGGARR21 pKa = 11.84PAGGRR26 pKa = 11.84PVGAGGHH33 pKa = 5.4GARR36 pKa = 11.84PGGGLAGIIGSLLSGRR52 pKa = 11.84GRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84GLL58 pKa = 3.26

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5254

0

5254

1607864

39

1859

306.0

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.696 ± 0.054

0.75 ± 0.009

6.437 ± 0.027

5.245 ± 0.031

2.568 ± 0.019

9.847 ± 0.037

2.208 ± 0.018

2.9 ± 0.025

1.258 ± 0.022

10.561 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.627 ± 0.011

1.361 ± 0.015

6.482 ± 0.036

2.296 ± 0.017

8.545 ± 0.034

4.429 ± 0.019

5.841 ± 0.021

9.816 ± 0.038

1.43 ± 0.013

1.702 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski