Micrococcus endophyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Micrococcus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8XRW7|A0A4Y8XRW7_9MICC ATP-binding cassette domain-containing protein (Fragment) OS=Micrococcus endophyticus OX=455343 GN=E4A41_14735 PE=4 SV=1
AA1 pKa = 7.32GAIAACEE8 pKa = 4.22CEE10 pKa = 4.8AGCPSCVQSPKK21 pKa = 10.18CGNRR25 pKa = 11.84NSPLDD30 pKa = 3.41KK31 pKa = 11.13AGALTVLRR39 pKa = 11.84FLLGQADD46 pKa = 3.79HH47 pKa = 6.69LVPVVAEE54 pKa = 4.56GSGEE58 pKa = 4.19TDD60 pKa = 2.67GGTDD64 pKa = 3.8DD65 pKa = 4.95GVGVDD70 pKa = 5.68DD71 pKa = 5.09EE72 pKa = 4.84DD73 pKa = 5.42ASGSGSSGTAA83 pKa = 2.89

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8ZDD0|A0A4Y8ZDD0_9MICC Dihydroorotase (Fragment) OS=Micrococcus endophyticus OX=455343 GN=E4A41_07490 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAAA34 pKa = 4.46

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2319

0

2319

377349

21

930

162.7

17.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.701 ± 0.081

0.59 ± 0.015

6.081 ± 0.051

6.397 ± 0.065

3.127 ± 0.041

8.873 ± 0.057

2.342 ± 0.033

3.758 ± 0.041

2.171 ± 0.044

10.183 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.028

1.877 ± 0.031

5.274 ± 0.046

3.017 ± 0.037

7.527 ± 0.062

5.06 ± 0.04

6.05 ± 0.047

9.247 ± 0.07

1.523 ± 0.028

1.994 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski