Amino acid dipepetide frequency for Halorubrum sp. SD626R

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.555AlaAla: 16.555 ± 0.17	0.726AlaCys: 0.726 ± 0.02	11.554AlaAsp: 11.554 ± 0.113	9.488AlaGlu: 9.488 ± 0.094	4.3AlaPhe: 4.3 ± 0.057	11.051AlaGly: 11.051 ± 0.109	1.87AlaHis: 1.87 ± 0.038	4.733AlaIle: 4.733 ± 0.062	1.79AlaLys: 1.79 ± 0.037	10.88AlaLeu: 10.88 ± 0.121	2.05AlaMet: 2.05 ± 0.031	2.395AlaAsn: 2.395 ± 0.041	4.26AlaPro: 4.26 ± 0.065	1.928AlaGln: 1.928 ± 0.036	6.619AlaArg: 6.619 ± 0.082	5.644AlaSer: 5.644 ± 0.069	7.093AlaThr: 7.093 ± 0.076	12.237AlaVal: 12.237 ± 0.118	1.153AlaTrp: 1.153 ± 0.026	2.836AlaTyr: 2.836 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.623CysAla: 0.623 ± 0.019	0.075CysCys: 0.075 ± 0.007	0.586CysAsp: 0.586 ± 0.02	0.578CysGlu: 0.578 ± 0.019	0.191CysPhe: 0.191 ± 0.011	0.834CysGly: 0.834 ± 0.026	0.163CysHis: 0.163 ± 0.01	0.206CysIle: 0.206 ± 0.013	0.108CysLys: 0.108 ± 0.009	0.485CysLeu: 0.485 ± 0.016	0.088CysMet: 0.088 ± 0.007	0.177CysAsn: 0.177 ± 0.011	0.529CysPro: 0.529 ± 0.02	0.132CysGln: 0.132 ± 0.011	0.444CysArg: 0.444 ± 0.017	0.384CysSer: 0.384 ± 0.017	0.368CysThr: 0.368 ± 0.017	0.478CysVal: 0.478 ± 0.018	0.081CysTrp: 0.081 ± 0.007	0.152CysTyr: 0.152 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	12.698AspAla: 12.698 ± 0.128	0.617AspCys: 0.617 ± 0.022	8.621AspAsp: 8.621 ± 0.099	8.394AspGlu: 8.394 ± 0.091	1.87AspPhe: 1.87 ± 0.033	9.336AspGly: 9.336 ± 0.12	1.759AspHis: 1.759 ± 0.034	2.718AspIle: 2.718 ± 0.046	0.918AspLys: 0.918 ± 0.027	7.427AspLeu: 7.427 ± 0.074	1.211AspMet: 1.211 ± 0.026	1.204AspAsn: 1.204 ± 0.033	5.392AspPro: 5.392 ± 0.073	1.524AspGln: 1.524 ± 0.037	7.745AspArg: 7.745 ± 0.081	3.462AspSer: 3.462 ± 0.051	3.839AspThr: 3.839 ± 0.052	7.931AspVal: 7.931 ± 0.078	0.967AspTrp: 0.967 ± 0.023	1.706AspTyr: 1.706 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	9.461GluAla: 9.461 ± 0.095	0.454GluCys: 0.454 ± 0.02	5.037GluAsp: 5.037 ± 0.067	6.585GluGlu: 6.585 ± 0.091	2.986GluPhe: 2.986 ± 0.045	5.932GluGly: 5.932 ± 0.065	1.861GluHis: 1.861 ± 0.036	3.764GluIle: 3.764 ± 0.059	1.725GluLys: 1.725 ± 0.038	7.256GluLeu: 7.256 ± 0.082	1.789GluMet: 1.789 ± 0.04	2.01GluAsn: 2.01 ± 0.041	4.23GluPro: 4.23 ± 0.057	2.067GluGln: 2.067 ± 0.044	7.928GluArg: 7.928 ± 0.097	5.284GluSer: 5.284 ± 0.065	6.481GluThr: 6.481 ± 0.069	5.993GluVal: 5.993 ± 0.067	1.108GluTrp: 1.108 ± 0.026	2.489GluTyr: 2.489 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	3.879PheAla: 3.879 ± 0.057	0.274PheCys: 0.274 ± 0.013	3.327PheAsp: 3.327 ± 0.049	3.019PheGlu: 3.019 ± 0.043	0.977PhePhe: 0.977 ± 0.031	3.213PheGly: 3.213 ± 0.048	0.604PheHis: 0.604 ± 0.02	0.994PheIle: 0.994 ± 0.032	0.426PheLys: 0.426 ± 0.018	2.698PheLeu: 2.698 ± 0.044	0.49PheMet: 0.49 ± 0.017	0.677PheAsn: 0.677 ± 0.022	1.319PhePro: 1.319 ± 0.028	0.682PheGln: 0.682 ± 0.022	1.844PheArg: 1.844 ± 0.032	1.611PheSer: 1.611 ± 0.035	1.774PheThr: 1.774 ± 0.035	3.16PheVal: 3.16 ± 0.057	0.347PheTrp: 0.347 ± 0.014	0.809PheTyr: 0.809 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	9.173GlyAla: 9.173 ± 0.103	0.63GlyCys: 0.63 ± 0.023	8.258GlyAsp: 8.258 ± 0.095	7.707GlyGlu: 7.707 ± 0.088	3.157GlyPhe: 3.157 ± 0.05	8.763GlyGly: 8.763 ± 0.1	1.639GlyHis: 1.639 ± 0.034	4.106GlyIle: 4.106 ± 0.053	1.753GlyLys: 1.753 ± 0.039	7.39GlyLeu: 7.39 ± 0.085	1.636GlyMet: 1.636 ± 0.034	1.911GlyAsn: 1.911 ± 0.036	3.636GlyPro: 3.636 ± 0.052	1.727GlyGln: 1.727 ± 0.035	5.345GlyArg: 5.345 ± 0.071	5.448GlySer: 5.448 ± 0.063	5.651GlyThr: 5.651 ± 0.058	8.41GlyVal: 8.41 ± 0.081	1.08GlyTrp: 1.08 ± 0.027	2.692GlyTyr: 2.692 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.128HisAla: 2.128 ± 0.041	0.169HisCys: 0.169 ± 0.01	1.697HisAsp: 1.697 ± 0.034	1.552HisGlu: 1.552 ± 0.029	0.518HisPhe: 0.518 ± 0.016	1.904HisGly: 1.904 ± 0.037	0.48HisHis: 0.48 ± 0.022	0.546HisIle: 0.546 ± 0.019	0.279HisLys: 0.279 ± 0.015	1.56HisLeu: 1.56 ± 0.032	0.275HisMet: 0.275 ± 0.014	0.456HisAsn: 0.456 ± 0.016	1.152HisPro: 1.152 ± 0.03	0.421HisGln: 0.421 ± 0.017	1.277HisArg: 1.277 ± 0.027	0.733HisSer: 0.733 ± 0.021	0.982HisThr: 0.982 ± 0.029	1.835HisVal: 1.835 ± 0.031	0.213HisTrp: 0.213 ± 0.013	0.496HisTyr: 0.496 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	4.883IleAla: 4.883 ± 0.057	0.258IleCys: 0.258 ± 0.013	4.108IleAsp: 4.108 ± 0.047	4.087IleGlu: 4.087 ± 0.059	0.816IlePhe: 0.816 ± 0.024	3.815IleGly: 3.815 ± 0.053	0.757IleHis: 0.757 ± 0.019	1.137IleIle: 1.137 ± 0.032	0.648IleLys: 0.648 ± 0.022	2.635IleLeu: 2.635 ± 0.049	0.481IleMet: 0.481 ± 0.018	0.961IleAsn: 0.961 ± 0.026	1.951IlePro: 1.951 ± 0.04	0.943IleGln: 0.943 ± 0.028	2.511IleArg: 2.511 ± 0.045	1.943IleSer: 1.943 ± 0.042	2.171IleThr: 2.171 ± 0.043	3.544IleVal: 3.544 ± 0.042	0.269IleTrp: 0.269 ± 0.012	0.778IleTyr: 0.778 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	1.758LysAla: 1.758 ± 0.037	0.1LysCys: 0.1 ± 0.009	0.917LysAsp: 0.917 ± 0.031	1.305LysGlu: 1.305 ± 0.036	0.475LysPhe: 0.475 ± 0.017	1.31LysGly: 1.31 ± 0.031	0.43LysHis: 0.43 ± 0.019	0.695LysIle: 0.695 ± 0.025	0.486LysLys: 0.486 ± 0.024	1.508LysLeu: 1.508 ± 0.033	0.344LysMet: 0.344 ± 0.014	0.521LysAsn: 0.521 ± 0.022	0.877LysPro: 0.877 ± 0.024	0.688LysGln: 0.688 ± 0.023	1.701LysArg: 1.701 ± 0.035	1.105LysSer: 1.105 ± 0.029	1.228LysThr: 1.228 ± 0.033	1.138LysVal: 1.138 ± 0.026	0.225LysTrp: 0.225 ± 0.013	0.491LysTyr: 0.491 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	11.332LeuAla: 11.332 ± 0.127	0.529LeuCys: 0.529 ± 0.019	7.998LeuAsp: 7.998 ± 0.079	5.579LeuGlu: 5.579 ± 0.074	2.936LeuPhe: 2.936 ± 0.052	7.602LeuGly: 7.602 ± 0.084	1.378LeuHis: 1.378 ± 0.029	3.062LeuIle: 3.062 ± 0.05	1.548LeuLys: 1.548 ± 0.033	7.907LeuLeu: 7.907 ± 0.111	1.232LeuMet: 1.232 ± 0.031	1.746LeuAsn: 1.746 ± 0.031	4.032LeuPro: 4.032 ± 0.051	1.737LeuGln: 1.737 ± 0.037	6.006LeuArg: 6.006 ± 0.063	5.499LeuSer: 5.499 ± 0.067	4.933LeuThr: 4.933 ± 0.062	7.987LeuVal: 7.987 ± 0.078	0.819LeuTrp: 0.819 ± 0.021	2.077LeuTyr: 2.077 ± 0.039	0.001LeuXaa: 0.001 ± 0.001
Met	1.722MetAla: 1.722 ± 0.035	0.118MetCys: 0.118 ± 0.008	1.171MetAsp: 1.171 ± 0.031	1.091MetGlu: 1.091 ± 0.03	0.475MetPhe: 0.475 ± 0.02	1.306MetGly: 1.306 ± 0.03	0.288MetHis: 0.288 ± 0.013	0.67MetIle: 0.67 ± 0.021	0.395MetLys: 0.395 ± 0.016	1.316MetLeu: 1.316 ± 0.03	0.271MetMet: 0.271 ± 0.015	0.554MetAsn: 0.554 ± 0.019	0.75MetPro: 0.75 ± 0.021	0.397MetGln: 0.397 ± 0.017	1.086MetArg: 1.086 ± 0.024	1.52MetSer: 1.52 ± 0.032	1.45MetThr: 1.45 ± 0.031	1.165MetVal: 1.165 ± 0.026	0.145MetTrp: 0.145 ± 0.011	0.398MetTyr: 0.398 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.616AsnAla: 2.616 ± 0.038	0.179AsnCys: 0.179 ± 0.013	1.619AsnAsp: 1.619 ± 0.029	1.715AsnGlu: 1.715 ± 0.035	0.619AsnPhe: 0.619 ± 0.021	1.88AsnGly: 1.88 ± 0.037	0.419AsnHis: 0.419 ± 0.016	0.831AsnIle: 0.831 ± 0.027	0.431AsnLys: 0.431 ± 0.02	1.841AsnLeu: 1.841 ± 0.036	0.36AsnMet: 0.36 ± 0.014	0.539AsnAsn: 0.539 ± 0.024	1.531AsnPro: 1.531 ± 0.03	0.559AsnGln: 0.559 ± 0.02	1.585AsnArg: 1.585 ± 0.031	0.922AsnSer: 0.922 ± 0.026	1.206AsnThr: 1.206 ± 0.034	2.23AsnVal: 2.23 ± 0.043	0.282AsnTrp: 0.282 ± 0.013	0.574AsnTyr: 0.574 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	5.215ProAla: 5.215 ± 0.071	0.239ProCys: 0.239 ± 0.013	5.355ProAsp: 5.355 ± 0.064	4.668ProGlu: 4.668 ± 0.054	1.607ProPhe: 1.607 ± 0.037	4.034ProGly: 4.034 ± 0.052	0.846ProHis: 0.846 ± 0.026	1.896ProIle: 1.896 ± 0.03	0.816ProLys: 0.816 ± 0.023	3.586ProLeu: 3.586 ± 0.053	0.77ProMet: 0.77 ± 0.024	1.117ProAsn: 1.117 ± 0.027	2.176ProPro: 2.176 ± 0.043	0.808ProGln: 0.808 ± 0.026	2.391ProArg: 2.391 ± 0.04	2.613ProSer: 2.613 ± 0.034	3.059ProThr: 3.059 ± 0.046	4.393ProVal: 4.393 ± 0.05	0.468ProTrp: 0.468 ± 0.017	1.091ProTyr: 1.091 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	2.066GlnAla: 2.066 ± 0.04	0.15GlnCys: 0.15 ± 0.01	1.122GlnAsp: 1.122 ± 0.03	1.543GlnGlu: 1.543 ± 0.039	0.886GlnPhe: 0.886 ± 0.028	1.484GlnGly: 1.484 ± 0.033	0.413GlnHis: 0.413 ± 0.019	1.035GlnIle: 1.035 ± 0.028	0.517GlnLys: 0.517 ± 0.021	2.019GlnLeu: 2.019 ± 0.041	0.472GlnMet: 0.472 ± 0.018	0.612GlnAsn: 0.612 ± 0.023	0.93GlnPro: 0.93 ± 0.024	0.842GlnGln: 0.842 ± 0.034	1.696GlnArg: 1.696 ± 0.035	1.34GlnSer: 1.34 ± 0.031	1.384GlnThr: 1.384 ± 0.036	1.578GlnVal: 1.578 ± 0.033	0.291GlnTrp: 0.291 ± 0.016	0.735GlnTyr: 0.735 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	7.031ArgAla: 7.031 ± 0.084	0.453ArgCys: 0.453 ± 0.017	5.778ArgAsp: 5.778 ± 0.056	7.234ArgGlu: 7.234 ± 0.079	2.476ArgPhe: 2.476 ± 0.041	5.084ArgGly: 5.084 ± 0.057	1.241ArgHis: 1.241 ± 0.026	3.112ArgIle: 3.112 ± 0.045	1.348ArgLys: 1.348 ± 0.031	6.421ArgLeu: 6.421 ± 0.063	1.221ArgMet: 1.221 ± 0.027	1.647ArgAsn: 1.647 ± 0.033	2.731ArgPro: 2.731 ± 0.045	1.625ArgGln: 1.625 ± 0.036	5.273ArgArg: 5.273 ± 0.072	3.667ArgSer: 3.667 ± 0.059	3.955ArgThr: 3.955 ± 0.052	5.944ArgVal: 5.944 ± 0.067	0.718ArgTrp: 0.718 ± 0.024	1.949ArgTyr: 1.949 ± 0.037	0.001ArgXaa: 0.001 ± 0.001
Ser	5.583SerAla: 5.583 ± 0.06	0.308SerCys: 0.308 ± 0.016	4.82SerAsp: 4.82 ± 0.053	4.16SerGlu: 4.16 ± 0.065	1.835SerPhe: 1.835 ± 0.038	5.501SerGly: 5.501 ± 0.057	0.951SerHis: 0.951 ± 0.025	2.239SerIle: 2.239 ± 0.039	1.131SerLys: 1.131 ± 0.027	4.605SerLeu: 4.605 ± 0.058	0.917SerMet: 0.917 ± 0.023	1.283SerAsn: 1.283 ± 0.035	2.647SerPro: 2.647 ± 0.038	1.189SerGln: 1.189 ± 0.031	3.302SerArg: 3.302 ± 0.049	2.65SerSer: 2.65 ± 0.045	3.306SerThr: 3.306 ± 0.054	4.916SerVal: 4.916 ± 0.063	0.56SerTrp: 0.56 ± 0.018	1.331SerTyr: 1.331 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	7.106ThrAla: 7.106 ± 0.085	0.377ThrCys: 0.377 ± 0.014	5.547ThrAsp: 5.547 ± 0.068	4.57ThrGlu: 4.57 ± 0.065	2.004ThrPhe: 2.004 ± 0.037	5.811ThrGly: 5.811 ± 0.067	1.139ThrHis: 1.139 ± 0.026	2.481ThrIle: 2.481 ± 0.046	0.948ThrLys: 0.948 ± 0.028	5.487ThrLeu: 5.487 ± 0.064	0.874ThrMet: 0.874 ± 0.024	1.322ThrAsn: 1.322 ± 0.033	3.101ThrPro: 3.101 ± 0.043	1.228ThrGln: 1.228 ± 0.032	3.454ThrArg: 3.454 ± 0.041	2.613ThrSer: 2.613 ± 0.04	3.927ThrThr: 3.927 ± 0.058	6.819ThrVal: 6.819 ± 0.072	0.565ThrTrp: 0.565 ± 0.02	1.609ThrTyr: 1.609 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	11.577ValAla: 11.577 ± 0.11	0.681ValCys: 0.681 ± 0.021	8.604ValAsp: 8.604 ± 0.091	7.991ValGlu: 7.991 ± 0.081	2.857ValPhe: 2.857 ± 0.044	8.225ValGly: 8.225 ± 0.084	1.645ValHis: 1.645 ± 0.033	3.291ValIle: 3.291 ± 0.046	1.368ValLys: 1.368 ± 0.032	7.385ValLeu: 7.385 ± 0.082	1.283ValMet: 1.283 ± 0.03	1.939ValAsn: 1.939 ± 0.038	4.29ValPro: 4.29 ± 0.058	1.677ValGln: 1.677 ± 0.031	5.958ValArg: 5.958 ± 0.075	5.18ValSer: 5.18 ± 0.064	6.096ValThr: 6.096 ± 0.064	9.106ValVal: 9.106 ± 0.089	0.784ValTrp: 0.784 ± 0.022	2.05ValTyr: 2.05 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	0.914TrpAla: 0.914 ± 0.027	0.114TrpCys: 0.114 ± 0.009	0.72TrpAsp: 0.72 ± 0.022	0.824TrpGlu: 0.824 ± 0.023	0.404TrpPhe: 0.404 ± 0.016	0.797TrpGly: 0.797 ± 0.021	0.211TrpHis: 0.211 ± 0.012	0.508TrpIle: 0.508 ± 0.018	0.247TrpLys: 0.247 ± 0.014	1.129TrpLeu: 1.129 ± 0.027	0.188TrpMet: 0.188 ± 0.012	0.303TrpAsn: 0.303 ± 0.013	0.422TrpPro: 0.422 ± 0.016	0.307TrpGln: 0.307 ± 0.015	0.859TrpArg: 0.859 ± 0.025	0.533TrpSer: 0.533 ± 0.021	0.713TrpThr: 0.713 ± 0.021	0.841TrpVal: 0.841 ± 0.022	0.162TrpTrp: 0.162 ± 0.01	0.344TrpTyr: 0.344 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.84TyrAla: 2.84 ± 0.044	0.228TyrCys: 0.228 ± 0.012	2.497TyrAsp: 2.497 ± 0.039	2.352TyrGlu: 2.352 ± 0.042	0.738TyrPhe: 0.738 ± 0.019	2.228TyrGly: 2.228 ± 0.042	0.579TyrHis: 0.579 ± 0.018	0.672TyrIle: 0.672 ± 0.021	0.421TyrLys: 0.421 ± 0.016	2.404TyrLeu: 2.404 ± 0.04	0.34TyrMet: 0.34 ± 0.016	0.562TyrAsn: 0.562 ± 0.022	1.193TyrPro: 1.193 ± 0.028	0.637TyrGln: 0.637 ± 0.022	1.885TyrArg: 1.885 ± 0.033	1.083TyrSer: 1.083 ± 0.026	1.366TyrThr: 1.366 ± 0.031	2.282TyrVal: 2.282 ± 0.036	0.302TyrTrp: 0.302 ± 0.013	0.773TyrTyr: 0.773 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 6274 proteins (1603608 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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