[Clostridium] methylpentosum DSM 5476

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillospiraceae incertae sedis; [Clostridium] methylpentosum

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3907 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0EIG0|C0EIG0_9FIRM Spermidine/putrescine import ATP-binding protein PotA OS=[Clostridium] methylpentosum DSM 5476 OX=537013 GN=potA PE=3 SV=1
MM1 pKa = 7.41NEE3 pKa = 3.41TWEE6 pKa = 4.56DD7 pKa = 3.62VAGDD11 pKa = 4.13ITDD14 pKa = 2.58KK15 pKa = 10.77WYY17 pKa = 8.9ITNVCIKK24 pKa = 10.43GFTNPLPEE32 pKa = 5.44SDD34 pKa = 3.54TAVASVRR41 pKa = 11.84FSEE44 pKa = 4.47MEE46 pKa = 4.09GPLADD51 pKa = 4.01GTDD54 pKa = 3.1ISLTAEE60 pKa = 4.08GAEE63 pKa = 4.09EE64 pKa = 4.11IYY66 pKa = 11.13YY67 pKa = 10.7SVDD70 pKa = 2.52GAAYY74 pKa = 9.64QKK76 pKa = 9.46YY77 pKa = 6.42TAPLTLDD84 pKa = 4.31FAQQDD89 pKa = 3.65AHH91 pKa = 5.97TVAAYY96 pKa = 9.94AVDD99 pKa = 3.51NGKK102 pKa = 9.71QGNTVEE108 pKa = 4.17KK109 pKa = 10.38VYY111 pKa = 10.64TKK113 pKa = 10.87AVAQLTDD120 pKa = 3.65LALKK124 pKa = 9.48YY125 pKa = 10.65DD126 pKa = 4.08GVVEE130 pKa = 4.11HH131 pKa = 7.32CEE133 pKa = 3.85TDD135 pKa = 3.1GLTKK139 pKa = 10.62HH140 pKa = 6.25FVYY143 pKa = 10.71LPNCADD149 pKa = 3.49NVQVMAQSADD159 pKa = 3.49KK160 pKa = 10.84IRR162 pKa = 11.84VNGQMLNSSDD172 pKa = 3.22WSDD175 pKa = 4.26GISLTPGEE183 pKa = 4.37TTEE186 pKa = 3.81ITIEE190 pKa = 4.01VTGSGKK196 pKa = 8.37TPSTYY201 pKa = 9.73TLEE204 pKa = 4.83AYY206 pKa = 10.4RR207 pKa = 11.84SMLIFDD213 pKa = 4.51YY214 pKa = 10.77EE215 pKa = 4.47AEE217 pKa = 4.34TVSYY221 pKa = 11.24DD222 pKa = 3.36DD223 pKa = 3.4TNYY226 pKa = 10.52TVMDD230 pKa = 4.06TQQNEE235 pKa = 4.32IKK237 pKa = 10.7SGDD240 pKa = 3.89SIAALISTEE249 pKa = 4.2EE250 pKa = 4.12KK251 pKa = 10.32TEE253 pKa = 4.02LTVTPIAGGDD263 pKa = 3.52NLIEE267 pKa = 4.0YY268 pKa = 9.05VPKK271 pKa = 10.53RR272 pKa = 11.84ILVSPTGINYY282 pKa = 10.21ALEE285 pKa = 4.37EE286 pKa = 4.29TDD288 pKa = 4.86SSFSDD293 pKa = 4.18LYY295 pKa = 11.15AYY297 pKa = 9.79SANADD302 pKa = 3.2MSEE305 pKa = 3.95AVQCEE310 pKa = 4.09YY311 pKa = 11.29GQTIPLTPGVDD322 pKa = 3.56VYY324 pKa = 10.78IQRR327 pKa = 11.84QATDD331 pKa = 3.52RR332 pKa = 11.84DD333 pKa = 4.49FISAIYY339 pKa = 9.42HH340 pKa = 5.74LEE342 pKa = 4.05VPRR345 pKa = 11.84RR346 pKa = 11.84PAAPEE351 pKa = 3.73VTAEE355 pKa = 4.35EE356 pKa = 4.23ITDD359 pKa = 3.64TSVTLQAMEE368 pKa = 4.28GALYY372 pKa = 10.61SAGGDD377 pKa = 3.39WQDD380 pKa = 3.3TPVFTDD386 pKa = 3.64LTPGTEE392 pKa = 4.15YY393 pKa = 10.63TFQVCLMATEE403 pKa = 4.0NAFRR407 pKa = 11.84SEE409 pKa = 3.82YY410 pKa = 10.37GAAKK414 pKa = 8.73ITTKK418 pKa = 10.14IPQVNPSDD426 pKa = 3.56YY427 pKa = 10.48SFEE430 pKa = 4.12VKK432 pKa = 10.71YY433 pKa = 11.12VDD435 pKa = 4.67GEE437 pKa = 4.54GNPVPGGGIISFEE450 pKa = 3.88EE451 pKa = 3.61VGPYY455 pKa = 9.68NRR457 pKa = 11.84EE458 pKa = 4.47DD459 pKa = 3.17IPLPYY464 pKa = 10.21GYY466 pKa = 8.53MQIIPAHH473 pKa = 6.85PDD475 pKa = 3.31EE476 pKa = 5.74DD477 pKa = 4.06WLFPTALEE485 pKa = 4.3WVDD488 pKa = 4.72GEE490 pKa = 4.47WKK492 pKa = 8.52VTNPVVEE499 pKa = 4.12IMVEE503 pKa = 3.97KK504 pKa = 9.62MASVDD509 pKa = 4.19IIFKK513 pKa = 9.99TPDD516 pKa = 3.05GNILEE521 pKa = 4.53DD522 pKa = 3.41WNYY525 pKa = 8.18TRR527 pKa = 11.84YY528 pKa = 10.06YY529 pKa = 10.24DD530 pKa = 4.04SEE532 pKa = 5.16GGGIEE537 pKa = 4.21TVPAPEE543 pKa = 4.89GYY545 pKa = 10.48EE546 pKa = 3.75FVGEE550 pKa = 3.7NTYY553 pKa = 10.93AVEE556 pKa = 4.48VIRR559 pKa = 11.84DD560 pKa = 3.63EE561 pKa = 4.28TGKK564 pKa = 10.75LVADD568 pKa = 4.18PSEE571 pKa = 3.92VTFWVKK577 pKa = 10.58EE578 pKa = 3.91IGSEE582 pKa = 4.29IPVTPSDD589 pKa = 3.49YY590 pKa = 11.03SFEE593 pKa = 4.1VKK595 pKa = 10.87YY596 pKa = 10.59IDD598 pKa = 4.83GEE600 pKa = 4.55GNPVPGGGTISFEE613 pKa = 3.83EE614 pKa = 4.06AGPYY618 pKa = 9.36NRR620 pKa = 11.84EE621 pKa = 4.74DD622 pKa = 3.13IPLPYY627 pKa = 10.21GYY629 pKa = 8.53MQIIPAHH636 pKa = 6.85PDD638 pKa = 3.31EE639 pKa = 5.76DD640 pKa = 3.98WLFPTSLVLVDD651 pKa = 4.98GEE653 pKa = 4.47WKK655 pKa = 8.46VTNPVVEE662 pKa = 4.12IMVEE666 pKa = 3.97KK667 pKa = 9.62MASVDD672 pKa = 5.05IIFQTPDD679 pKa = 2.95GNILEE684 pKa = 4.53DD685 pKa = 3.41WNYY688 pKa = 8.18TRR690 pKa = 11.84YY691 pKa = 10.06YY692 pKa = 10.24DD693 pKa = 4.04SEE695 pKa = 5.16GGGIEE700 pKa = 4.21TVPAPEE706 pKa = 4.77GYY708 pKa = 10.55EE709 pKa = 3.95FIGEE713 pKa = 3.68NTYY716 pKa = 10.89AVEE719 pKa = 4.48VIRR722 pKa = 11.84DD723 pKa = 3.63EE724 pKa = 4.28TGKK727 pKa = 10.75LVADD731 pKa = 4.06PSEE734 pKa = 4.12VIFIVKK740 pKa = 10.42AIDD743 pKa = 3.4TEE745 pKa = 4.88GPSDD749 pKa = 4.01PEE751 pKa = 4.22EE752 pKa = 4.38PSTPGGTNTPEE763 pKa = 4.34GNSPQTGDD771 pKa = 4.34DD772 pKa = 3.45SHH774 pKa = 8.32ASNLLLLILVSAGTLLLVTIIRR796 pKa = 11.84NQKK799 pKa = 8.33KK800 pKa = 9.75KK801 pKa = 11.06EE802 pKa = 3.95MTTEE806 pKa = 3.89EE807 pKa = 4.12

Molecular weight: 88.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0ED61|C0ED61_9FIRM UDP-galactose 4-epimerase OS=[Clostridium] methylpentosum DSM 5476 OX=537013 GN=CLOSTMETH_01787 PE=3 SV=1
MM1 pKa = 7.58LFFLRR6 pKa = 11.84FFISIHH12 pKa = 4.67TRR14 pKa = 11.84KK15 pKa = 8.61GTATRR20 pKa = 11.84RR21 pKa = 11.84GLSIAQTFLTISIHH35 pKa = 4.47TRR37 pKa = 11.84KK38 pKa = 8.8GTVTSRR44 pKa = 11.84SPGQIQRR51 pKa = 11.84AFSSIHH57 pKa = 4.49TRR59 pKa = 11.84KK60 pKa = 10.55GPGQLL65 pKa = 3.57

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3907

0

3907

988130

34

2451

252.9

28.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.249 ± 0.052

1.532 ± 0.022

5.58 ± 0.037

6.65 ± 0.043

4.258 ± 0.035

7.115 ± 0.039

1.732 ± 0.021

6.385 ± 0.041

5.662 ± 0.047

9.458 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.018

4.234 ± 0.034

3.845 ± 0.028

4.066 ± 0.03

4.803 ± 0.044

6.535 ± 0.036

5.732 ± 0.047

6.794 ± 0.032

1.013 ± 0.018

3.803 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski