Clostridium sp. CAG:352

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6RUW9|R6RUW9_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:352 OX=1262798 GN=BN621_00524 PE=4 SV=1
MM1 pKa = 7.52IKK3 pKa = 10.49DD4 pKa = 3.73KK5 pKa = 11.42SCLFKK10 pKa = 10.96DD11 pKa = 4.21IIEE14 pKa = 4.34DD15 pKa = 3.38MKK17 pKa = 11.24NIYY20 pKa = 10.61LEE22 pKa = 4.45FGDD25 pKa = 4.32EE26 pKa = 5.17KK27 pKa = 11.12LIEE30 pKa = 4.87DD31 pKa = 5.27DD32 pKa = 4.02DD33 pKa = 3.92WCIYY37 pKa = 8.32TVEE40 pKa = 4.58EE41 pKa = 4.18KK42 pKa = 11.06LEE44 pKa = 4.64PDD46 pKa = 3.54TEE48 pKa = 4.78CYY50 pKa = 10.57VFDD53 pKa = 4.13YY54 pKa = 10.81PDD56 pKa = 3.83YY57 pKa = 11.27DD58 pKa = 4.17DD59 pKa = 3.95EE60 pKa = 4.66TGEE63 pKa = 4.4EE64 pKa = 4.29ILPEE68 pKa = 4.11YY69 pKa = 10.05AVKK72 pKa = 10.16HH73 pKa = 4.55QMKK76 pKa = 10.43CVTTCEE82 pKa = 3.96ALQDD86 pKa = 3.9VIANSLSQRR95 pKa = 11.84SDD97 pKa = 3.18LTVEE101 pKa = 3.92QLIDD105 pKa = 3.32AVNYY109 pKa = 10.25YY110 pKa = 10.08FDD112 pKa = 5.6KK113 pKa = 10.64IAFMEE118 pKa = 4.57FF119 pKa = 2.98

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6R665|R6R665_9CLOT Phosphomethylpyrimidine kinase OS=Clostridium sp. CAG:352 OX=1262798 GN=BN621_01579 PE=3 SV=1
MM1 pKa = 7.59TISEE5 pKa = 4.21MLGFCFGIIIACFLAFAIVSCIYY28 pKa = 10.54YY29 pKa = 9.7FLIKK33 pKa = 10.48DD34 pKa = 3.09ILRR37 pKa = 11.84IVRR40 pKa = 11.84RR41 pKa = 11.84FMRR44 pKa = 11.84ARR46 pKa = 11.84RR47 pKa = 11.84IRR49 pKa = 11.84LASKK53 pKa = 10.37KK54 pKa = 10.45

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2097

0

2097

632363

29

3030

301.6

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.092 ± 0.056

1.521 ± 0.02

5.834 ± 0.045

7.181 ± 0.059

4.255 ± 0.036

6.48 ± 0.056

1.448 ± 0.027

8.578 ± 0.055

7.857 ± 0.041

8.4 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.023

5.447 ± 0.051

3.136 ± 0.034

2.622 ± 0.029

3.884 ± 0.045

6.909 ± 0.06

5.57 ± 0.046

6.314 ± 0.041

0.709 ± 0.017

4.089 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski