Avon-Heathcote Estuary associated kieseladnavirus (AHEaBV) (Avon-Heathcote Estuary associated bacilladnavirus)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Baphyvirales; Bacilladnaviridae; Kieseladnavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1P8YT84|A0A1P8YT84_AHEBV p3 OS=Avon-Heathcote Estuary associated kieseladnavirus OX=2169964 PE=4 SV=1
MM1 pKa = 7.41CRR3 pKa = 11.84ASSMDD8 pKa = 3.47SPTLRR13 pKa = 11.84TSQVNEE19 pKa = 3.62FLNFGDD25 pKa = 4.37RR26 pKa = 11.84NVHH29 pKa = 5.08VDD31 pKa = 3.63NIVRR35 pKa = 11.84LLDD38 pKa = 3.65FLVDD42 pKa = 4.07GGSFTPQQADD52 pKa = 4.21LYY54 pKa = 9.21WNALYY59 pKa = 10.35EE60 pKa = 4.51HH61 pKa = 6.49YY62 pKa = 10.58HH63 pKa = 6.53HH64 pKa = 7.02GVLPGMEE71 pKa = 4.44RR72 pKa = 11.84FPLPGIDD79 pKa = 3.34RR80 pKa = 11.84EE81 pKa = 4.67LYY83 pKa = 9.88FLPALTVLFDD93 pKa = 4.0NYY95 pKa = 10.16IDD97 pKa = 4.89PPTQLEE103 pKa = 4.31SIRR106 pKa = 11.84IEE108 pKa = 3.69WSEE111 pKa = 4.1EE112 pKa = 3.84VLSDD116 pKa = 3.71TDD118 pKa = 3.83SLTDD122 pKa = 3.63SEE124 pKa = 5.55LSDD127 pKa = 3.97LVGDD131 pKa = 4.91IIDD134 pKa = 4.39PDD136 pKa = 4.16SVIDD140 pKa = 3.72EE141 pKa = 4.61CLLAVINGEE150 pKa = 4.1LGDD153 pKa = 3.69VGGDD157 pKa = 3.33VGQDD161 pKa = 3.67DD162 pKa = 5.76LSDD165 pKa = 4.02ASDD168 pKa = 3.74GTDD171 pKa = 2.86TLFF174 pKa = 4.91

Molecular weight:
19.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|A0A1P8YT88|CAPSD_AHEBV Capsid protein OS=Avon-Heathcote Estuary associated kieseladnavirus OX=2169964 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84RR3 pKa = 11.84TRR5 pKa = 11.84SRR7 pKa = 11.84SWTPGTPPRR16 pKa = 11.84KK17 pKa = 9.4KK18 pKa = 10.14SEE20 pKa = 3.96RR21 pKa = 11.84CHH23 pKa = 5.98VVSRR27 pKa = 11.84DD28 pKa = 3.36PARR31 pKa = 11.84RR32 pKa = 11.84LPWSRR37 pKa = 11.84NRR39 pKa = 11.84TDD41 pKa = 4.0ARR43 pKa = 11.84PSQAKK48 pKa = 9.05PVSKK52 pKa = 10.66KK53 pKa = 8.84PTATALMRR61 pKa = 11.84IPTVQAVKK69 pKa = 10.6KK70 pKa = 9.09EE71 pKa = 3.94YY72 pKa = 8.83HH73 pKa = 5.54TALTVKK79 pKa = 10.21QQDD82 pKa = 3.05KK83 pKa = 10.57RR84 pKa = 11.84RR85 pKa = 11.84EE86 pKa = 4.31SKK88 pKa = 10.47FKK90 pKa = 10.09QALRR94 pKa = 11.84EE95 pKa = 4.12TKK97 pKa = 9.78RR98 pKa = 11.84VKK100 pKa = 10.56DD101 pKa = 3.49SIMRR105 pKa = 11.84GIEE108 pKa = 3.86YY109 pKa = 7.42TAHH112 pKa = 5.67VAGEE116 pKa = 4.02VSKK119 pKa = 11.14VVEE122 pKa = 4.13PLALGGSAAQPEE134 pKa = 4.59MAEE137 pKa = 4.14FLLPAAGIADD147 pKa = 3.79VVKK150 pKa = 10.34RR151 pKa = 11.84KK152 pKa = 9.49AAIIEE157 pKa = 4.29KK158 pKa = 8.44TAKK161 pKa = 9.99AAQGDD166 pKa = 3.57RR167 pKa = 11.84DD168 pKa = 3.98AYY170 pKa = 10.13KK171 pKa = 10.82SLTDD175 pKa = 3.11TGKK178 pKa = 8.31YY179 pKa = 8.31QKK181 pKa = 10.35MMLEE185 pKa = 4.18AKK187 pKa = 9.82NRR189 pKa = 11.84PKK191 pKa = 10.91VNLIEE196 pKa = 4.05YY197 pKa = 9.52VPRR200 pKa = 11.84AEE202 pKa = 4.4DD203 pKa = 3.46LQIEE207 pKa = 4.61MIDD210 pKa = 3.3

Molecular weight:
23.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1462

174

474

292.4

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.524 ± 0.893

1.984 ± 0.569

7.182 ± 1.275

5.267 ± 0.396

4.856 ± 0.839

5.609 ± 0.923

2.804 ± 0.421

4.993 ± 0.265

5.472 ± 1.304

8.96 ± 1.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.615

3.967 ± 1.198

4.925 ± 0.855

3.557 ± 0.764

6.019 ± 0.881

7.25 ± 0.486

7.182 ± 0.573

5.814 ± 0.463

2.052 ± 0.454

2.052 ± 0.261

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski