Halomonas sp. PA5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4FNX0|A0A6M4FNX0_9GAMM Acylphosphatase OS=Halomonas sp. PA5 OX=2730357 GN=HIO72_16250 PE=3 SV=1
MM1 pKa = 7.7PRR3 pKa = 11.84TLPNRR8 pKa = 11.84SILAATLSTTLLATALSAPMTQAQEE33 pKa = 3.72RR34 pKa = 11.84DD35 pKa = 3.59QFSVCWSIYY44 pKa = 9.54AGWMPWAYY52 pKa = 10.68ADD54 pKa = 4.53IEE56 pKa = 4.67GIVDD60 pKa = 3.57KK61 pKa = 10.6WADD64 pKa = 3.59EE65 pKa = 4.13YY66 pKa = 11.54GISIDD71 pKa = 3.78VIQVNDD77 pKa = 3.61YY78 pKa = 11.01VEE80 pKa = 5.11SINQFTSGNVDD91 pKa = 3.21GCAMTNMDD99 pKa = 5.17ALTIPAASGVDD110 pKa = 3.58STALIINDD118 pKa = 3.79YY119 pKa = 11.35SDD121 pKa = 4.12GNDD124 pKa = 4.04GIVLKK129 pKa = 11.11NSDD132 pKa = 4.49DD133 pKa = 3.81LADD136 pKa = 3.49IEE138 pKa = 4.33GRR140 pKa = 11.84RR141 pKa = 11.84VNLVQFSVSHH151 pKa = 5.55YY152 pKa = 8.95FLARR156 pKa = 11.84ALEE159 pKa = 4.48SVGLTEE165 pKa = 5.89RR166 pKa = 11.84DD167 pKa = 3.11IEE169 pKa = 4.52TVNTSDD175 pKa = 3.85ADD177 pKa = 3.6IVGLFSSASTEE188 pKa = 5.17AVAAWNPQLSAIADD202 pKa = 3.8MPDD205 pKa = 3.29SNVVYY210 pKa = 9.59TSAEE214 pKa = 3.85IPGEE218 pKa = 4.05ILDD221 pKa = 3.86MMVVNTDD228 pKa = 3.31TLAANPDD235 pKa = 3.58FGHH238 pKa = 6.83ALVGAWYY245 pKa = 9.75EE246 pKa = 4.12VMALVEE252 pKa = 4.49ARR254 pKa = 11.84DD255 pKa = 3.84EE256 pKa = 4.13QALSIMADD264 pKa = 3.14AAGTDD269 pKa = 3.62LEE271 pKa = 5.34GYY273 pKa = 9.63LAQLDD278 pKa = 3.86ATYY281 pKa = 10.47LYY283 pKa = 10.01TDD285 pKa = 3.55PTEE288 pKa = 5.49AIALMEE294 pKa = 4.51GDD296 pKa = 4.18EE297 pKa = 4.87LLEE300 pKa = 4.04TMQRR304 pKa = 11.84VAEE307 pKa = 4.28FSFEE311 pKa = 4.11HH312 pKa = 6.7GLLGDD317 pKa = 4.01MAPDD321 pKa = 4.04PGVVGVEE328 pKa = 4.5TPSGVYY334 pKa = 10.35GDD336 pKa = 4.77EE337 pKa = 4.3SNIMLRR343 pKa = 11.84FDD345 pKa = 3.72PSYY348 pKa = 8.2TQAYY352 pKa = 8.53IDD354 pKa = 3.71SRR356 pKa = 11.84QQ357 pKa = 3.03

Molecular weight: 38.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4FKT5|A0A6M4FKT5_9GAMM Exodeoxyribonuclease 7 large subunit OS=Halomonas sp. PA5 OX=2730357 GN=xseA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3407

0

3407

1107370

23

2263

325.0

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.004 ± 0.046

0.955 ± 0.013

5.446 ± 0.041

6.68 ± 0.047

3.501 ± 0.026

8.158 ± 0.043

2.469 ± 0.022

4.987 ± 0.03

2.639 ± 0.032

11.556 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.02

2.58 ± 0.022

4.873 ± 0.025

3.89 ± 0.032

7.272 ± 0.043

5.635 ± 0.028

4.868 ± 0.025

7.027 ± 0.037

1.442 ± 0.019

2.388 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski