Clostridium phage phiCD6356

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2ELJ4|E2ELJ4_9CAUD Uncharacterized protein OS=Clostridium phage phiCD6356 OX=864178 GN=phiCD6356_14 PE=4 SV=1
MM1 pKa = 7.71FLFEE5 pKa = 5.52KK6 pKa = 9.32NWSMTDD12 pKa = 3.09IDD14 pKa = 4.43NMDD17 pKa = 2.93IFYY20 pKa = 10.94YY21 pKa = 10.83LDD23 pKa = 2.95ILVYY27 pKa = 10.0MKK29 pKa = 10.55EE30 pKa = 4.03NKK32 pKa = 9.46KK33 pKa = 10.92DD34 pKa = 3.73NNNPNEE40 pKa = 3.87NDD42 pKa = 3.49VYY44 pKa = 10.71IDD46 pKa = 3.65QISWLL51 pKa = 3.87

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2ELL5|E2ELL5_9CAUD Helix-turn-helix domain protein OS=Clostridium phage phiCD6356 OX=864178 GN=phiCD6356_35 PE=4 SV=1
MM1 pKa = 8.26DD2 pKa = 4.88NFLLNILAGVIASLIFYY19 pKa = 9.98IISKK23 pKa = 9.74LFRR26 pKa = 11.84QVKK29 pKa = 8.87NHH31 pKa = 5.97SAQKK35 pKa = 10.51SGWEE39 pKa = 3.7FDD41 pKa = 4.05LKK43 pKa = 10.85IKK45 pKa = 10.36FKK47 pKa = 10.76KK48 pKa = 10.25SRR50 pKa = 3.35

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

10709

44

1733

181.5

20.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.416 ± 0.861

1.149 ± 0.234

5.78 ± 0.353

9.721 ± 0.459

4.09 ± 0.369

4.846 ± 0.249

0.962 ± 0.17

8.806 ± 0.441

11.635 ± 0.486

8.404 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.17

7.256 ± 0.371

1.578 ± 0.18

2.549 ± 0.338

3.306 ± 0.221

6.107 ± 0.469

5.5 ± 0.335

5.435 ± 0.218

0.943 ± 0.116

4.118 ± 0.507
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski