Pseudoxanthomonas indica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5LPR8|A0A1T5LPR8_9GAMM DUF418 domain-containing protein OS=Pseudoxanthomonas indica OX=428993 GN=SAMN06296058_2879 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.78PVKK5 pKa = 10.37LSRR8 pKa = 11.84ALTAALLLAVPFVSYY23 pKa = 10.34AQEE26 pKa = 3.74AAEE29 pKa = 4.39PEE31 pKa = 4.5EE32 pKa = 4.41ASSPVTWSIAATTDD46 pKa = 3.25YY47 pKa = 11.1VFRR50 pKa = 11.84GVSQTDD56 pKa = 3.25EE57 pKa = 4.69GPALQAGLTYY67 pKa = 8.9TAPFGLYY74 pKa = 9.45VGAWGSNVDD83 pKa = 5.32FGDD86 pKa = 4.44PDD88 pKa = 3.6PEE90 pKa = 5.75AGVRR94 pKa = 11.84GVDD97 pKa = 3.41TEE99 pKa = 4.48LDD101 pKa = 3.65LFVGYY106 pKa = 10.33SHH108 pKa = 7.95DD109 pKa = 6.39FNDD112 pKa = 3.19SWNLDD117 pKa = 3.58VSALRR122 pKa = 11.84YY123 pKa = 7.19TYY125 pKa = 10.34HH126 pKa = 6.67GASDD130 pKa = 4.21GVDD133 pKa = 2.88SGDD136 pKa = 3.45YY137 pKa = 9.36MEE139 pKa = 5.85YY140 pKa = 9.47IAKK143 pKa = 7.97VTWAGPVEE151 pKa = 4.42VSGLVAYY158 pKa = 10.29APDD161 pKa = 4.05YY162 pKa = 11.78GDD164 pKa = 3.96LTPKK168 pKa = 10.6AKK170 pKa = 10.23EE171 pKa = 4.0SYY173 pKa = 10.69ANLSASYY180 pKa = 10.28EE181 pKa = 4.18FGDD184 pKa = 4.15SGISLGAGVGYY195 pKa = 9.29TSVDD199 pKa = 3.51YY200 pKa = 10.81GAEE203 pKa = 4.2TIDD206 pKa = 4.75GEE208 pKa = 4.29RR209 pKa = 11.84FDD211 pKa = 5.45YY212 pKa = 11.14GVTDD216 pKa = 3.88YY217 pKa = 11.4FDD219 pKa = 4.01GALTVSKK226 pKa = 10.97AFGPATATLGYY237 pKa = 8.22YY238 pKa = 7.15TTFASDD244 pKa = 3.62ADD246 pKa = 4.15LLKK249 pKa = 10.94DD250 pKa = 3.43EE251 pKa = 4.97SGIYY255 pKa = 9.77DD256 pKa = 3.82DD257 pKa = 6.32RR258 pKa = 11.84IALTISIGNN267 pKa = 3.66

Molecular weight: 28.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5LAN8|A0A1T5LAN8_9GAMM Membrane-bound lytic murein transglycosylase B OS=Pseudoxanthomonas indica OX=428993 GN=SAMN06296058_2206 PE=4 SV=1
MM1 pKa = 7.25LTLASQFDD9 pKa = 4.16SRR11 pKa = 11.84HH12 pKa = 5.58APLLPLDD19 pKa = 3.2RR20 pKa = 11.84WLRR23 pKa = 11.84RR24 pKa = 11.84MVVCGLLIVLLFPLGRR40 pKa = 11.84GQIPWLGWAPLWLVGMPLAAWWALHH65 pKa = 6.45RR66 pKa = 11.84FRR68 pKa = 11.84LPWPRR73 pKa = 11.84RR74 pKa = 11.84GAWTGRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84LGGQARR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84APMPAHH98 pKa = 6.59WASHH102 pKa = 5.58GARR105 pKa = 11.84ATT107 pKa = 3.44

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3555

0

3555

1197874

30

5474

337.0

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.586 ± 0.061

0.78 ± 0.013

5.832 ± 0.03

5.328 ± 0.044

3.412 ± 0.024

8.469 ± 0.056

2.226 ± 0.02

4.195 ± 0.029

2.9 ± 0.037

10.741 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.02

2.698 ± 0.031

5.205 ± 0.034

4.121 ± 0.023

7.377 ± 0.04

5.528 ± 0.035

5.014 ± 0.039

7.359 ± 0.035

1.635 ± 0.018

2.445 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski