Streptomyces guanduensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0R6U3|A0A1H0R6U3_9ACTN Long-chain acyl-CoA synthetase OS=Streptomyces guanduensis OX=310781 GN=SAMN05216259_12116 PE=4 SV=1
MM1 pKa = 7.03TRR3 pKa = 11.84SGPTATVRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84LAALLTGLALAATGVADD30 pKa = 4.24LTTGEE35 pKa = 4.16AAAAAPAGPLLYY47 pKa = 10.73VADD50 pKa = 4.38SGSGDD55 pKa = 3.45VSVVDD60 pKa = 4.57GGSDD64 pKa = 3.2TATSSVSVGGSPQGVAVSPDD84 pKa = 2.96GATAYY89 pKa = 9.13VTDD92 pKa = 3.66ATGNTVSVIDD102 pKa = 3.89TATDD106 pKa = 3.38TVTATVPVGAIPYY119 pKa = 8.83SAAVTPDD126 pKa = 2.85GSEE129 pKa = 4.2VYY131 pKa = 10.67VADD134 pKa = 4.69LGANAVSVIDD144 pKa = 3.84TATDD148 pKa = 3.36TVTATIPAGVAPVTVAVNPAGTQVYY173 pKa = 7.18VTNDD177 pKa = 3.04SEE179 pKa = 4.64TNAVQVISTATHH191 pKa = 6.6TLTSSIPVRR200 pKa = 11.84AHH202 pKa = 5.91SYY204 pKa = 10.47GVAFGQGGALAYY216 pKa = 10.48VSLVNGALVAVIDD229 pKa = 4.14TATGTVTSDD238 pKa = 2.87IPVGVLPEE246 pKa = 4.27GVAVSPDD253 pKa = 3.14GTRR256 pKa = 11.84VYY258 pKa = 10.81VADD261 pKa = 4.43GNTDD265 pKa = 3.24AVSVISAATGTVTATVPVGPSPQGVAFDD293 pKa = 4.5PSGSRR298 pKa = 11.84AYY300 pKa = 9.54VTDD303 pKa = 3.75SASDD307 pKa = 3.55TVTVIDD313 pKa = 3.97TATSTVASTVTVGSHH328 pKa = 6.24PAGIAAVPVPPRR340 pKa = 11.84ADD342 pKa = 3.21LGVSLTATGVPGLVNGRR359 pKa = 11.84IDD361 pKa = 3.7YY362 pKa = 8.11TVTITDD368 pKa = 4.17HH369 pKa = 6.6GPSALASGTVTATLPPSTTATSNSCDD395 pKa = 3.25ATGSRR400 pKa = 11.84VTCTVGPLAAGASTTRR416 pKa = 11.84TFSVPVGLLTVGLPYY431 pKa = 10.38TVTATRR437 pKa = 11.84TGSSPADD444 pKa = 3.71PNTADD449 pKa = 3.48DD450 pKa = 3.99TATRR454 pKa = 11.84TCTVVTSLVITCSS467 pKa = 3.15

Molecular weight: 45.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9XWA0|A0A1G9XWA0_9ACTN Uncharacterized protein OS=Streptomyces guanduensis OX=310781 GN=SAMN05216259_102306 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6769

0

6769

2347717

26

10692

346.8

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.694 ± 0.049

0.738 ± 0.01

5.838 ± 0.022

5.234 ± 0.033

2.628 ± 0.016

9.845 ± 0.032

2.338 ± 0.016

2.894 ± 0.02

1.773 ± 0.02

10.023 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.603 ± 0.012

1.723 ± 0.019

6.565 ± 0.035

2.917 ± 0.018

7.905 ± 0.044

5.171 ± 0.032

6.304 ± 0.041

8.252 ± 0.027

1.479 ± 0.013

2.073 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski