Lily symptomless virus (LSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q70WL7|Q70WL7_LSV Helicase OS=Lily symptomless virus OX=12173 PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 4.06VLLGLLSEE10 pKa = 5.08FGFEE14 pKa = 4.05RR15 pKa = 11.84LSSEE19 pKa = 3.79LSLPIVVHH27 pKa = 5.74SVPGGGKK34 pKa = 9.59SSLIRR39 pKa = 11.84KK40 pKa = 8.43LINKK44 pKa = 8.62DD45 pKa = 3.29RR46 pKa = 11.84RR47 pKa = 11.84FSAYY51 pKa = 10.57SFGLEE56 pKa = 4.02DD57 pKa = 4.36CEE59 pKa = 5.14SITGVRR65 pKa = 11.84IKK67 pKa = 10.16KK68 pKa = 9.61AHH70 pKa = 5.83ASIPRR75 pKa = 11.84SEE77 pKa = 3.76FVVFDD82 pKa = 3.96EE83 pKa = 4.94YY84 pKa = 11.0IEE86 pKa = 4.86GDD88 pKa = 3.49APPWAFAVFADD99 pKa = 4.59PLQGGPGPVLRR110 pKa = 11.84AHH112 pKa = 6.76FIKK115 pKa = 10.45RR116 pKa = 11.84RR117 pKa = 11.84SHH119 pKa = 6.76RR120 pKa = 11.84FGKK123 pKa = 8.64CTAQLLNDD131 pKa = 4.03LSYY134 pKa = 11.06EE135 pKa = 4.21VEE137 pKa = 4.03SDD139 pKa = 3.43LADD142 pKa = 3.39VVQIQGLYY150 pKa = 8.62EE151 pKa = 3.96TDD153 pKa = 3.32LQGTVVYY160 pKa = 10.36YY161 pKa = 7.52EE162 pKa = 3.94ACIGNLLRR170 pKa = 11.84AHH172 pKa = 6.75SVPAYY177 pKa = 9.64CISEE181 pKa = 3.72IRR183 pKa = 11.84GQTFEE188 pKa = 4.48SVTFVTSEE196 pKa = 3.9NYY198 pKa = 9.85PVDD201 pKa = 3.38RR202 pKa = 11.84ALAFQCLTRR211 pKa = 11.84HH212 pKa = 6.01RR213 pKa = 11.84SSLLILSPNATYY225 pKa = 7.59TTSS228 pKa = 2.83

Molecular weight: 25.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q70WL3|Q70WL3_LSV Capsid protein OS=Lily symptomless virus OX=12173 PE=3 SV=1
MM1 pKa = 7.18SVWGARR7 pKa = 11.84KK8 pKa = 8.95PNTPVGYY15 pKa = 10.15KK16 pKa = 8.38EE17 pKa = 4.58LKK19 pKa = 9.56SSEE22 pKa = 4.37IIDD25 pKa = 3.79TQIMDD30 pKa = 3.65EE31 pKa = 4.0ALKK34 pKa = 10.71RR35 pKa = 11.84RR36 pKa = 11.84TTIVLCLLSAFPRR49 pKa = 11.84DD50 pKa = 3.08ICRR53 pKa = 11.84DD54 pKa = 3.03ILRR57 pKa = 11.84RR58 pKa = 11.84TSSHH62 pKa = 5.15IVGLGRR68 pKa = 11.84SRR70 pKa = 11.84YY71 pKa = 8.46ARR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84ALQIGRR82 pKa = 11.84CEE84 pKa = 3.45RR85 pKa = 11.84CYY87 pKa = 10.27RR88 pKa = 11.84VYY90 pKa = 10.82PPVCGSKK97 pKa = 10.5CDD99 pKa = 5.53NKK101 pKa = 10.27TCWPGLSINTNVANHH116 pKa = 6.88IDD118 pKa = 3.54HH119 pKa = 6.86GVTEE123 pKa = 4.71VIPWISPHH131 pKa = 6.89RR132 pKa = 11.84GQFYY136 pKa = 10.69LRR138 pKa = 11.84PKK140 pKa = 10.15

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2777

64

1948

462.8

52.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.994 ± 0.693

2.809 ± 0.373

4.429 ± 0.514

6.626 ± 0.77

4.609 ± 0.678

6.194 ± 0.384

2.413 ± 0.249

5.978 ± 0.383

5.257 ± 1.132

9.903 ± 0.782

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.389

4.285 ± 0.745

4.789 ± 0.541

3.133 ± 0.261

6.554 ± 0.976

6.698 ± 0.866

5.618 ± 0.266

5.726 ± 0.94

1.152 ± 0.193

3.637 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski