Sulfitobacter phage EE36phi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Aorunvirus; Sulfitobacter virus EE36phi1

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4NTB5|C4NTB5_9CAUD Uncharacterized protein OS=Sulfitobacter phage EE36phi1 OX=490913 PE=4 SV=1
MM1 pKa = 8.13SDD3 pKa = 3.1LTLNAVFGPQYY14 pKa = 10.48EE15 pKa = 4.22IVITNDD21 pKa = 3.09TVGVSDD27 pKa = 5.06NFDD30 pKa = 3.32LTLEE34 pKa = 4.04VSEE37 pKa = 4.62PLRR40 pKa = 11.84IAVGTGYY47 pKa = 10.84NINQTSTGTLDD58 pKa = 3.23VRR60 pKa = 11.84IIALEE65 pKa = 3.65PVGAYY70 pKa = 9.76RR71 pKa = 11.84AVGYY75 pKa = 10.52DD76 pKa = 3.51GMYY79 pKa = 7.89TQPDD83 pKa = 3.88VDD85 pKa = 4.36SLAAYY90 pKa = 10.25AGVTRR95 pKa = 11.84MATVAGDD102 pKa = 4.2PINVVRR108 pKa = 11.84SGLMTEE114 pKa = 5.48GAWNWTSNAPIFIAANGVLTQTQPTAGNPVRR145 pKa = 11.84RR146 pKa = 11.84IGWAISATEE155 pKa = 4.46LNLDD159 pKa = 4.3PYY161 pKa = 10.47PIIGVV166 pKa = 3.65

Molecular weight: 17.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4NTA5|C4NTA5_9CAUD Uncharacterized protein OS=Sulfitobacter phage EE36phi1 OX=490913 PE=4 SV=1
MM1 pKa = 7.44LVLKK5 pKa = 10.9LEE7 pKa = 4.18LHH9 pKa = 5.72SARR12 pKa = 11.84TGEE15 pKa = 3.76ITEE18 pKa = 3.97IGRR21 pKa = 11.84TIIANVGGTSEE32 pKa = 4.68LGNYY36 pKa = 8.14LCKK39 pKa = 10.24VARR42 pKa = 11.84KK43 pKa = 9.31QPDD46 pKa = 3.08GSEE49 pKa = 4.02YY50 pKa = 11.26SNTKK54 pKa = 6.53TWKK57 pKa = 9.61SPLRR61 pKa = 11.84TGTITNYY68 pKa = 9.68PRR70 pKa = 11.84LRR72 pKa = 11.84YY73 pKa = 9.27NVWRR77 pKa = 11.84LVIRR81 pKa = 11.84SLLAAFPEE89 pKa = 4.28EE90 pKa = 4.25RR91 pKa = 11.84KK92 pKa = 10.26

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

23000

35

3785

291.1

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.0 ± 0.409

0.77 ± 0.138

6.443 ± 0.156

6.735 ± 0.249

3.839 ± 0.165

7.309 ± 0.278

1.865 ± 0.19

5.661 ± 0.157

5.604 ± 0.371

7.83 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.257 ± 0.158

5.265 ± 0.202

4.322 ± 0.185

4.422 ± 0.172

5.061 ± 0.203

6.135 ± 0.142

6.213 ± 0.197

6.6 ± 0.246

1.413 ± 0.152

3.257 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski