Exophiala oligosperma

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12793 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2DRA5|A0A0D2DRA5_9EURO Uncharacterized protein OS=Exophiala oligosperma OX=215243 GN=PV06_09258 PE=4 SV=1
MM1 pKa = 7.19QRR3 pKa = 11.84TLALAALAAVAFANPMPQGVTSAIAPEE30 pKa = 4.04ASAPAGCQSTWPGTFEE46 pKa = 3.64ITVVNVTSSGKK57 pKa = 9.55RR58 pKa = 11.84DD59 pKa = 3.25LEE61 pKa = 3.96KK62 pKa = 10.55RR63 pKa = 11.84QKK65 pKa = 11.12DD66 pKa = 3.66GVLTLTLADD75 pKa = 4.54GVLKK79 pKa = 10.73DD80 pKa = 3.32QAGRR84 pKa = 11.84TGYY87 pKa = 9.86IASNYY92 pKa = 8.11QFQFDD97 pKa = 4.83DD98 pKa = 4.46PPQNGAIYY106 pKa = 9.01TKK108 pKa = 10.65GFSVCSNYY116 pKa = 10.85SLALGGDD123 pKa = 3.96ALWYY127 pKa = 9.73QCLSGSFYY135 pKa = 11.47NLYY138 pKa = 9.79NKK140 pKa = 9.74NWANTCNAIYY150 pKa = 9.67IYY152 pKa = 11.25AMSGSSAPATQAADD166 pKa = 3.98GQPQATPAPAPPVSQVSDD184 pKa = 3.81GQPQATTGVPVTQISDD200 pKa = 3.78GQPQATTGAPVSQISDD216 pKa = 3.86GQPQAPTGAPVSQISDD232 pKa = 3.86GQPQAPTGAPVSQISDD248 pKa = 3.86GQPQAPTGAPVSQISDD264 pKa = 3.86GQPQAPTGAPVSQISDD280 pKa = 3.86GQPQAPTGAPVSQISDD296 pKa = 3.86GQPQAPTGAPVSQISDD312 pKa = 3.86GQPQAPTGAPVSQISDD328 pKa = 3.86GQPQAPTGAPVSQISDD344 pKa = 3.86GQPQAPTGAPVSQISDD360 pKa = 3.86GQPQAPTGAPVSQISDD376 pKa = 3.86GQPQAPTGAPVSQISDD392 pKa = 3.86GQPQAPTGAPVSQISDD408 pKa = 3.86GQPQAPTATVEE419 pKa = 4.03AASNTGAAPATYY431 pKa = 9.07TGAANRR437 pKa = 11.84EE438 pKa = 4.17GLSGSLAVVAGLAGAMALLL457 pKa = 4.29

Molecular weight: 45.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2DQB2|A0A0D2DQB2_9EURO Plasma membrane fusion protein PRM1 OS=Exophiala oligosperma OX=215243 GN=PV06_03431 PE=3 SV=1
MM1 pKa = 7.3VLGLGKK7 pKa = 8.57TRR9 pKa = 11.84SRR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 5.7PGGTTVTTTTTTTHH27 pKa = 6.4HH28 pKa = 6.8PAGTGAGGVARR39 pKa = 11.84KK40 pKa = 6.21PTLMQRR46 pKa = 11.84LRR48 pKa = 11.84GRR50 pKa = 11.84TTPRR54 pKa = 11.84STATTGRR61 pKa = 11.84APRR64 pKa = 11.84RR65 pKa = 11.84TRR67 pKa = 11.84RR68 pKa = 11.84TRR70 pKa = 11.84AAPATHH76 pKa = 7.03HH77 pKa = 6.19KK78 pKa = 10.05RR79 pKa = 11.84KK80 pKa = 8.33TSIGDD85 pKa = 3.67KK86 pKa = 10.84VSGAMLKK93 pKa = 10.71LKK95 pKa = 10.64GSVTHH100 pKa = 7.14RR101 pKa = 11.84PGEE104 pKa = 4.09KK105 pKa = 9.75AAGTRR110 pKa = 11.84RR111 pKa = 11.84MHH113 pKa = 5.63GTDD116 pKa = 2.88GKK118 pKa = 9.59GSRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84FYY125 pKa = 11.48

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11880

913

12793

6246231

49

5476

488.3

54.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.377 ± 0.019

1.257 ± 0.009

5.782 ± 0.014

5.973 ± 0.02

3.74 ± 0.012

6.809 ± 0.021

2.479 ± 0.01

4.835 ± 0.014

4.722 ± 0.017

8.874 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.008

3.656 ± 0.011

5.959 ± 0.021

4.144 ± 0.017

6.196 ± 0.018

8.392 ± 0.026

6.171 ± 0.016

6.168 ± 0.014

1.457 ± 0.008

2.753 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski