Parasporobacterium paucivorans DSM 15970

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Parasporobacterium; Parasporobacterium paucivorans

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6FS24|A0A1M6FS24_9FIRM Inner membrane protein OS=Parasporobacterium paucivorans DSM 15970 OX=1122934 GN=SAMN02745691_01184 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.38KK3 pKa = 10.2IITALLLVTTLLTLGACGNSSASGSNEE30 pKa = 3.87TTSTASGKK38 pKa = 9.77DD39 pKa = 3.28RR40 pKa = 11.84PVLRR44 pKa = 11.84VGMEE48 pKa = 4.12CAYY51 pKa = 10.58APYY54 pKa = 10.67NWAQSDD60 pKa = 3.9DD61 pKa = 4.15SNGAVPIVDD70 pKa = 3.74SSNYY74 pKa = 9.87AYY76 pKa = 10.38GYY78 pKa = 10.47DD79 pKa = 3.44VMMAKK84 pKa = 10.51YY85 pKa = 8.46IADD88 pKa = 3.76YY89 pKa = 10.48IGYY92 pKa = 9.54DD93 pKa = 3.45LEE95 pKa = 4.61IYY97 pKa = 10.33KK98 pKa = 9.84IDD100 pKa = 3.85WDD102 pKa = 4.25SLPLAVQSGTIDD114 pKa = 3.51CVIAGQSITAEE125 pKa = 4.02RR126 pKa = 11.84QQTVDD131 pKa = 4.19FSTPYY136 pKa = 9.51YY137 pKa = 8.92YY138 pKa = 11.2ASIVALTTKK147 pKa = 10.49DD148 pKa = 3.39SPYY151 pKa = 11.25ASAKK155 pKa = 10.03GLSDD159 pKa = 4.3LAGATCTSQLSTVWYY174 pKa = 9.98NDD176 pKa = 3.66CLPQIKK182 pKa = 9.77DD183 pKa = 3.7ANILAAMEE191 pKa = 4.31SAPAMMTALDD201 pKa = 3.81SGKK204 pKa = 10.57VNLVVTDD211 pKa = 3.87MPTALAALQVYY222 pKa = 10.91DD223 pKa = 4.94NFTLLDD229 pKa = 4.01FNQTDD234 pKa = 4.04DD235 pKa = 3.86NFSVSDD241 pKa = 3.61EE242 pKa = 4.53EE243 pKa = 4.27INIGISLKK251 pKa = 10.59KK252 pKa = 10.63GNSEE256 pKa = 4.5LLGLINGALATLTEE270 pKa = 4.85DD271 pKa = 4.07DD272 pKa = 4.78FKK274 pKa = 11.28TMMEE278 pKa = 4.2EE279 pKa = 4.4AISVQPLSEE288 pKa = 3.96

Molecular weight: 30.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6BBH2|A0A1M6BBH2_9FIRM Methionine--tRNA ligase OS=Parasporobacterium paucivorans DSM 15970 OX=1122934 GN=metG PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTVGGRR28 pKa = 11.84KK29 pKa = 9.04VIAARR34 pKa = 11.84RR35 pKa = 11.84EE36 pKa = 3.96KK37 pKa = 10.63GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2361

0

2361

740729

40

2936

313.7

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.247 ± 0.046

1.373 ± 0.025

5.545 ± 0.037

7.416 ± 0.052

4.275 ± 0.038

7.311 ± 0.049

1.654 ± 0.02

8.313 ± 0.055

6.79 ± 0.039

8.998 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.111 ± 0.027

4.56 ± 0.039

3.366 ± 0.029

2.776 ± 0.024

4.439 ± 0.036

6.156 ± 0.041

5.249 ± 0.037

6.824 ± 0.042

0.791 ± 0.016

3.806 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski