Hubei sobemo-like virus 48

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEH0|A0A1L3KEH0_9VIRU Capsid protein OS=Hubei sobemo-like virus 48 OX=1923236 PE=3 SV=1
MM1 pKa = 6.84EE2 pKa = 3.96QAYY5 pKa = 10.6SPVVWSLPDD14 pKa = 4.27DD15 pKa = 4.23FDD17 pKa = 4.55SRR19 pKa = 11.84TRR21 pKa = 11.84FDD23 pKa = 3.01WAIRR27 pKa = 11.84RR28 pKa = 11.84LDD30 pKa = 3.7MQSSPGMPYY39 pKa = 9.35MRR41 pKa = 11.84EE42 pKa = 3.79APTNGKK48 pKa = 6.63WLKK51 pKa = 9.55WDD53 pKa = 3.39GVEE56 pKa = 4.11YY57 pKa = 10.93DD58 pKa = 3.65AMQANRR64 pKa = 11.84LWHH67 pKa = 7.05DD68 pKa = 3.87VQCVLSDD75 pKa = 3.42DD76 pKa = 3.94WEE78 pKa = 4.15HH79 pKa = 6.8VIRR82 pKa = 11.84VFIKK86 pKa = 9.76QEE88 pKa = 3.49PHH90 pKa = 6.4KK91 pKa = 10.22KK92 pKa = 9.3HH93 pKa = 6.39KK94 pKa = 10.47ARR96 pKa = 11.84EE97 pKa = 4.26GRR99 pKa = 11.84WRR101 pKa = 11.84LIMASSLPVQLVWHH115 pKa = 6.0MLFSYY120 pKa = 10.53MNDD123 pKa = 3.45LEE125 pKa = 4.98ISEE128 pKa = 5.41CYY130 pKa = 10.13NIPSQHH136 pKa = 5.71GLILVGGGWKK146 pKa = 10.2DD147 pKa = 3.61YY148 pKa = 10.58LRR150 pKa = 11.84SWKK153 pKa = 10.54EE154 pKa = 3.46EE155 pKa = 3.91GLSVGLDD162 pKa = 3.12KK163 pKa = 11.37SAWDD167 pKa = 3.23WTAPRR172 pKa = 11.84WVMDD176 pKa = 3.14WDD178 pKa = 3.86LDD180 pKa = 3.39FRR182 pKa = 11.84YY183 pKa = 10.57RR184 pKa = 11.84MGRR187 pKa = 11.84GKK189 pKa = 10.61RR190 pKa = 11.84MEE192 pKa = 3.93EE193 pKa = 3.4WHH195 pKa = 6.81RR196 pKa = 11.84LAKK199 pKa = 10.5LMYY202 pKa = 9.63HH203 pKa = 6.78HH204 pKa = 6.74MFDD207 pKa = 4.26HH208 pKa = 7.22PVLQLSDD215 pKa = 3.59GTLLRR220 pKa = 11.84QTVPGIMKK228 pKa = 9.74SGCVNTISTNGHH240 pKa = 5.42AQVMMHH246 pKa = 6.29CVVAEE251 pKa = 4.2DD252 pKa = 4.03SNVPYY257 pKa = 10.44EE258 pKa = 4.87PYY260 pKa = 10.42PKK262 pKa = 9.74TCGDD266 pKa = 3.85DD267 pKa = 3.7TLEE270 pKa = 5.04HH271 pKa = 6.64PMHH274 pKa = 5.85TQSLEE279 pKa = 3.66YY280 pKa = 9.01YY281 pKa = 8.33GRR283 pKa = 11.84YY284 pKa = 8.76GVVVKK289 pKa = 10.63SVSEE293 pKa = 3.71NMEE296 pKa = 4.12FVGHH300 pKa = 6.8EE301 pKa = 4.17FTDD304 pKa = 3.94SGPHH308 pKa = 6.24PLYY311 pKa = 10.24ISKK314 pKa = 10.18HH315 pKa = 4.23MKK317 pKa = 9.49KK318 pKa = 9.95LQYY321 pKa = 10.49LADD324 pKa = 5.27DD325 pKa = 5.62IIPDD329 pKa = 4.12FLDD332 pKa = 3.18AMARR336 pKa = 11.84MYY338 pKa = 11.09VHH340 pKa = 6.62TRR342 pKa = 11.84YY343 pKa = 10.01FSIWEE348 pKa = 4.02DD349 pKa = 3.29LAIVNGTPLPMSKK362 pKa = 8.88MAYY365 pKa = 9.42RR366 pKa = 11.84YY367 pKa = 9.17WYY369 pKa = 9.74DD370 pKa = 3.4FSVV373 pKa = 3.05

Molecular weight: 43.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEG3|A0A1L3KEG3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 48 OX=1923236 PE=4 SV=1
MM1 pKa = 7.19SWKK4 pKa = 10.45DD5 pKa = 3.5YY6 pKa = 10.95LLVSGGVYY14 pKa = 9.88GLWMGHH20 pKa = 6.39KK21 pKa = 9.6IGVGSKK27 pKa = 8.42ISAVAGMVIPGFRR40 pKa = 11.84KK41 pKa = 10.13LKK43 pKa = 8.03WAVGRR48 pKa = 11.84AEE50 pKa = 4.64VVSQPAASNHH60 pKa = 5.44LRR62 pKa = 11.84LEE64 pKa = 4.02SRR66 pKa = 11.84KK67 pKa = 10.44DD68 pKa = 3.32GSEE71 pKa = 4.06EE72 pKa = 3.96VSLTAPRR79 pKa = 11.84YY80 pKa = 8.94QVIVCEE86 pKa = 4.39KK87 pKa = 10.17KK88 pKa = 10.32DD89 pKa = 3.54GRR91 pKa = 11.84LVKK94 pKa = 10.44LGCAVRR100 pKa = 11.84FDD102 pKa = 4.3GNFLVGPDD110 pKa = 3.86HH111 pKa = 7.14VLGEE115 pKa = 4.36ADD117 pKa = 3.61LPKK120 pKa = 10.61YY121 pKa = 10.57AVGRR125 pKa = 11.84QKK127 pKa = 10.27FVCLDD132 pKa = 3.22KK133 pKa = 11.22KK134 pKa = 10.87EE135 pKa = 4.71RR136 pKa = 11.84IPLDD140 pKa = 3.36TDD142 pKa = 3.92LVAIRR147 pKa = 11.84MSPSEE152 pKa = 4.14LSTIGVSVAKK162 pKa = 10.25IGLVSAKK169 pKa = 9.97GVYY172 pKa = 9.18SQIVGVDD179 pKa = 3.45SKK181 pKa = 11.32GTTGVLKK188 pKa = 9.71MDD190 pKa = 3.9RR191 pKa = 11.84VAFGRR196 pKa = 11.84TVYY199 pKa = 10.82DD200 pKa = 3.52GTTLPGYY207 pKa = 10.27SGAAYY212 pKa = 9.29TSGAFCSAIHH222 pKa = 5.63QSGGAVNGGYY232 pKa = 10.31ASSYY236 pKa = 9.79IWMLLKK242 pKa = 10.83DD243 pKa = 3.96HH244 pKa = 6.84LRR246 pKa = 11.84VEE248 pKa = 4.74DD249 pKa = 3.8VVEE252 pKa = 4.28PEE254 pKa = 5.09SKK256 pKa = 10.84KK257 pKa = 10.5NWNSDD262 pKa = 3.23TPEE265 pKa = 3.74WLLSQFKK272 pKa = 10.48AGKK275 pKa = 7.92KK276 pKa = 9.66LKK278 pKa = 9.24WKK280 pKa = 9.21RR281 pKa = 11.84TGDD284 pKa = 3.57PDD286 pKa = 5.6LIEE289 pKa = 5.74LMLDD293 pKa = 3.23DD294 pKa = 5.03GKK296 pKa = 11.0FSRR299 pKa = 11.84VSPASMHH306 pKa = 6.43KK307 pKa = 10.68AFGPTWHH314 pKa = 7.13DD315 pKa = 2.78HH316 pKa = 6.63DD317 pKa = 5.17VIEE320 pKa = 4.79KK321 pKa = 10.64GFDD324 pKa = 2.69RR325 pKa = 11.84SYY327 pKa = 11.05RR328 pKa = 11.84DD329 pKa = 3.33VPRR332 pKa = 11.84EE333 pKa = 4.0SILEE337 pKa = 4.12SVPCTSGSGEE347 pKa = 4.01DD348 pKa = 4.25HH349 pKa = 6.79GSKK352 pKa = 10.81LPGALSLLEE361 pKa = 4.91PDD363 pKa = 3.77QAWARR368 pKa = 11.84LSLQQEE374 pKa = 3.73MRR376 pKa = 11.84EE377 pKa = 3.9FFNLSKK383 pKa = 10.55RR384 pKa = 11.84QQEE387 pKa = 4.57DD388 pKa = 2.7IRR390 pKa = 11.84KK391 pKa = 8.69CSQRR395 pKa = 11.84QMSQNLASSGQAKK408 pKa = 8.86TMATKK413 pKa = 10.77NSS415 pKa = 3.54

Molecular weight: 45.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1252

373

464

417.3

46.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.188 ± 1.037

1.038 ± 0.24

6.31 ± 0.55

5.032 ± 0.131

3.355 ± 0.292

7.907 ± 0.86

2.636 ± 0.773

4.313 ± 0.425

5.99 ± 0.751

8.626 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.355 ± 0.985

2.316 ± 0.15

5.112 ± 0.392

3.914 ± 0.336

5.751 ± 0.082

8.706 ± 0.68

3.994 ± 0.295

7.987 ± 0.268

2.875 ± 0.584

3.514 ± 0.644
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski