Actinomyces phage xhp1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CIC9|A0A2P1CIC9_9CAUD Tape measure protein OS=Actinomyces phage xhp1 OX=2099653 GN=XHP1_00017 PE=3 SV=1
MM1 pKa = 7.0SAVVAVTIGLVLAVAAIPVIVCVVYY26 pKa = 8.82LTGIYY31 pKa = 10.41AGDD34 pKa = 3.63ALDD37 pKa = 4.93RR38 pKa = 11.84LVCMGLDD45 pKa = 3.27AGDD48 pKa = 5.27RR49 pKa = 11.84IAEE52 pKa = 4.41MIDD55 pKa = 3.49GEE57 pKa = 4.83VPGKK61 pKa = 10.46

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CIF4|A0A2P1CIF4_9CAUD Uncharacterized protein OS=Actinomyces phage xhp1 OX=2099653 GN=XHP1_00025 PE=4 SV=1
MM1 pKa = 7.66PKK3 pKa = 10.07KK4 pKa = 10.26PPLDD8 pKa = 3.7VEE10 pKa = 3.96ITRR13 pKa = 11.84AIARR17 pKa = 11.84AHH19 pKa = 6.01ILYY22 pKa = 10.42ANRR25 pKa = 11.84PHH27 pKa = 7.78IDD29 pKa = 3.32EE30 pKa = 4.38RR31 pKa = 11.84VHH33 pKa = 7.49RR34 pKa = 11.84LVTEE38 pKa = 4.42ACARR42 pKa = 11.84AAHH45 pKa = 6.0YY46 pKa = 9.36PVEE49 pKa = 4.47VKK51 pKa = 10.57PSRR54 pKa = 11.84SALAPMRR61 pKa = 11.84PRR63 pKa = 11.84RR64 pKa = 11.84SAA66 pKa = 2.91

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10860

33

1229

201.1

21.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.744 ± 0.47

0.875 ± 0.146

5.939 ± 0.252

6.197 ± 0.365

2.422 ± 0.24

8.49 ± 0.493

1.63 ± 0.188

4.273 ± 0.266

3.702 ± 0.26

8.223 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.952 ± 0.113

2.044 ± 0.211

5.82 ± 0.336

3.702 ± 0.154

6.998 ± 0.553

5.792 ± 0.3

7.265 ± 0.464

7.532 ± 0.34

2.164 ± 0.177

2.238 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski