Granulicella tundricola (strain ATCC BAA-1859 / DSM 23138 / MP5ACTX9)

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Granulicella; Granulicella tundricola

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8X0E3|E8X0E3_GRATM Cold-shock DNA-binding domain protein OS=Granulicella tundricola (strain ATCC BAA-1859 / DSM 23138 / MP5ACTX9) OX=1198114 GN=AciX9_0737 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 4.04ISAIVGTEE10 pKa = 3.9LKK12 pKa = 10.55SVAFVEE18 pKa = 4.96DD19 pKa = 4.03FLEE22 pKa = 4.3LRR24 pKa = 11.84FGTPCLTLYY33 pKa = 10.36DD34 pKa = 4.19WPHH37 pKa = 5.85VLLPDD42 pKa = 4.06FSVAYY47 pKa = 10.11GEE49 pKa = 4.14PEE51 pKa = 3.95YY52 pKa = 11.23RR53 pKa = 11.84NALCAQINEE62 pKa = 4.46EE63 pKa = 4.12VVEE66 pKa = 4.24ASLEE70 pKa = 4.21EE71 pKa = 4.67GDD73 pKa = 4.32SLTIEE78 pKa = 4.23LANGVVFGLSLRR90 pKa = 11.84EE91 pKa = 3.87EE92 pKa = 4.58DD93 pKa = 3.41VDD95 pKa = 4.24GPEE98 pKa = 4.19SGSFSEE104 pKa = 4.46TGQAVDD110 pKa = 3.29AQEE113 pKa = 3.77FF114 pKa = 3.65

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8WWV4|E8WWV4_GRATM Uncharacterized protein OS=Granulicella tundricola (strain ATCC BAA-1859 / DSM 23138 / MP5ACTX9) OX=1198114 GN=AciX9_0360 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.55THH17 pKa = 5.47GFLTRR22 pKa = 11.84MKK24 pKa = 9.03TKK26 pKa = 10.72AGAAVLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4514

0

4514

1572207

30

3375

348.3

37.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.055 ± 0.044

0.847 ± 0.012

5.033 ± 0.03

5.287 ± 0.05

3.873 ± 0.023

8.112 ± 0.048

2.279 ± 0.019

4.97 ± 0.029

3.777 ± 0.032

10.048 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.019

3.379 ± 0.035

5.442 ± 0.03

3.838 ± 0.027

5.742 ± 0.048

6.368 ± 0.042

6.659 ± 0.067

7.123 ± 0.037

1.262 ± 0.018

2.685 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski