Prevotella sp. CAG:592

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6WQT2|R6WQT2_9BACT DNA-directed RNA polymerase sigma subunit RpoE OS=Prevotella sp. CAG:592 OX=1262931 GN=BN725_00662 PE=3 SV=1
MM1 pKa = 7.48AKK3 pKa = 10.21VIEE6 pKa = 4.17GLYY9 pKa = 9.12YY10 pKa = 10.57AEE12 pKa = 3.69SHH14 pKa = 6.58EE15 pKa = 4.29YY16 pKa = 10.57VKK18 pKa = 11.22VEE20 pKa = 4.28GEE22 pKa = 3.74FGYY25 pKa = 10.31IGITDD30 pKa = 3.75YY31 pKa = 11.5AQNALGNIVYY41 pKa = 10.46VDD43 pKa = 3.82MPDD46 pKa = 3.25TDD48 pKa = 4.97DD49 pKa = 3.59EE50 pKa = 4.83VEE52 pKa = 3.96AGEE55 pKa = 4.77DD56 pKa = 3.55FGAVEE61 pKa = 4.41SVKK64 pKa = 10.58AASDD68 pKa = 3.41LTSPVSGTVVEE79 pKa = 4.42VNEE82 pKa = 4.22ALEE85 pKa = 4.2DD86 pKa = 3.63TPEE89 pKa = 5.41IINQDD94 pKa = 3.13AFANWIIKK102 pKa = 10.1VEE104 pKa = 4.06LSDD107 pKa = 4.33KK108 pKa = 11.13TEE110 pKa = 4.57LDD112 pKa = 3.53NLMDD116 pKa = 4.36AAAYY120 pKa = 10.43AEE122 pKa = 4.29FCEE125 pKa = 4.74KK126 pKa = 10.05EE127 pKa = 3.8AHH129 pKa = 6.03

Molecular weight: 14.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6XI84|R6XI84_9BACT Tetrahydrofolate synthase OS=Prevotella sp. CAG:592 OX=1262931 GN=BN725_00381 PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2159

0

2159

749068

29

3058

347.0

38.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.601 ± 0.054

1.409 ± 0.024

6.04 ± 0.039

6.188 ± 0.05

4.372 ± 0.032

7.043 ± 0.046

1.911 ± 0.024

6.578 ± 0.046

6.857 ± 0.045

8.477 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.157 ± 0.03

4.975 ± 0.039

3.547 ± 0.028

3.232 ± 0.03

4.695 ± 0.045

6.027 ± 0.045

5.767 ± 0.051

6.806 ± 0.043

1.165 ± 0.02

4.152 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski