Amino acid dipepetide frequency for Anncaliia algerae PRA109

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.268AlaAla: 1.268 ± 0.053
0.559AlaCys: 0.559 ± 0.023
1.403AlaAsp: 1.403 ± 0.037
1.867AlaGlu: 1.867 ± 0.049
1.828AlaPhe: 1.828 ± 0.039
1.116AlaGly: 1.116 ± 0.037
0.572AlaHis: 0.572 ± 0.03
2.796AlaIle: 2.796 ± 0.05
2.738AlaLys: 2.738 ± 0.054
3.424AlaLeu: 3.424 ± 0.059
0.715AlaMet: 0.715 ± 0.031
1.924AlaAsn: 1.924 ± 0.045
0.738AlaPro: 0.738 ± 0.048
0.915AlaGln: 0.915 ± 0.031
1.013AlaArg: 1.013 ± 0.036
2.121AlaSer: 2.121 ± 0.041
1.347AlaThr: 1.347 ± 0.036
1.602AlaVal: 1.602 ± 0.043
0.212AlaTrp: 0.212 ± 0.014
1.371AlaTyr: 1.371 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
0.552CysAla: 0.552 ± 0.023
0.393CysCys: 0.393 ± 0.02
1.011CysAsp: 1.011 ± 0.034
1.05CysGlu: 1.05 ± 0.036
1.287CysPhe: 1.287 ± 0.036
0.796CysGly: 0.796 ± 0.028
0.409CysHis: 0.409 ± 0.023
1.823CysIle: 1.823 ± 0.044
1.991CysLys: 1.991 ± 0.044
1.828CysLeu: 1.828 ± 0.039
0.373CysMet: 0.373 ± 0.019
1.342CysAsn: 1.342 ± 0.041
0.438CysPro: 0.438 ± 0.026
0.386CysGln: 0.386 ± 0.019
0.718CysArg: 0.718 ± 0.03
1.403CysSer: 1.403 ± 0.036
0.884CysThr: 0.884 ± 0.03
0.894CysVal: 0.894 ± 0.034
0.172CysTrp: 0.172 ± 0.012
0.926CysTyr: 0.926 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
1.603AspAla: 1.603 ± 0.038
0.96AspCys: 0.96 ± 0.033
2.88AspAsp: 2.88 ± 0.059
4.334AspGlu: 4.334 ± 0.073
3.391AspPhe: 3.391 ± 0.058
1.725AspGly: 1.725 ± 0.049
0.726AspHis: 0.726 ± 0.029
4.746AspIle: 4.746 ± 0.075
5.277AspLys: 5.277 ± 0.074
5.451AspLeu: 5.451 ± 0.077
0.907AspMet: 0.907 ± 0.033
3.454AspAsn: 3.454 ± 0.068
1.268AspPro: 1.268 ± 0.038
1.168AspGln: 1.168 ± 0.032
1.44AspArg: 1.44 ± 0.039
3.732AspSer: 3.732 ± 0.058
2.286AspThr: 2.286 ± 0.047
2.308AspVal: 2.308 ± 0.056
0.322AspTrp: 0.322 ± 0.018
2.389AspTyr: 2.389 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
2.178GluAla: 2.178 ± 0.045
1.367GluCys: 1.367 ± 0.039
3.737GluAsp: 3.737 ± 0.061
7.843GluGlu: 7.843 ± 0.129
4.125GluPhe: 4.125 ± 0.066
2.554GluGly: 2.554 ± 0.054
1.127GluHis: 1.127 ± 0.036
8.052GluIle: 8.052 ± 0.095
7.745GluLys: 7.745 ± 0.113
6.376GluLeu: 6.376 ± 0.075
1.848GluMet: 1.848 ± 0.045
6.909GluAsn: 6.909 ± 0.082
1.184GluPro: 1.184 ± 0.039
1.656GluGln: 1.656 ± 0.054
2.95GluArg: 2.95 ± 0.05
5.202GluSer: 5.202 ± 0.084
3.305GluThr: 3.305 ± 0.053
3.731GluVal: 3.731 ± 0.067
0.355GluTrp: 0.355 ± 0.018
3.405GluTyr: 3.405 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
1.847PheAla: 1.847 ± 0.052
1.335PheCys: 1.335 ± 0.041
3.383PheAsp: 3.383 ± 0.054
3.362PheGlu: 3.362 ± 0.058
4.396PhePhe: 4.396 ± 0.084
2.103PheGly: 2.103 ± 0.05
1.094PheHis: 1.094 ± 0.036
6.727PheIle: 6.727 ± 0.091
5.772PheLys: 5.772 ± 0.082
7.284PheLeu: 7.284 ± 0.101
1.419PheMet: 1.419 ± 0.036
4.923PheAsn: 4.923 ± 0.078
1.336PhePro: 1.336 ± 0.036
1.226PheGln: 1.226 ± 0.036
1.925PheArg: 1.925 ± 0.044
4.55PheSer: 4.55 ± 0.072
3.153PheThr: 3.153 ± 0.055
3.503PheVal: 3.503 ± 0.061
0.338PheTrp: 0.338 ± 0.021
3.888PheTyr: 3.888 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
1.155GlyAla: 1.155 ± 0.036
0.678GlyCys: 0.678 ± 0.03
1.471GlyAsp: 1.471 ± 0.041
2.052GlyGlu: 2.052 ± 0.05
1.957GlyPhe: 1.957 ± 0.044
1.801GlyGly: 1.801 ± 0.081
0.727GlyHis: 0.727 ± 0.03
3.586GlyIle: 3.586 ± 0.065
3.432GlyLys: 3.432 ± 0.06
3.121GlyLeu: 3.121 ± 0.053
0.821GlyMet: 0.821 ± 0.032
2.356GlyAsn: 2.356 ± 0.088
0.794GlyPro: 0.794 ± 0.027
0.664GlyGln: 0.664 ± 0.035
1.534GlyArg: 1.534 ± 0.049
2.582GlySer: 2.582 ± 0.067
1.784GlyThr: 1.784 ± 0.049
2.03GlyVal: 2.03 ± 0.055
0.247GlyTrp: 0.247 ± 0.014
1.831GlyTyr: 1.831 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
0.453HisAla: 0.453 ± 0.022
0.4HisCys: 0.4 ± 0.019
0.768HisAsp: 0.768 ± 0.028
1.134HisGlu: 1.134 ± 0.036
1.202HisPhe: 1.202 ± 0.033
0.7HisGly: 0.7 ± 0.029
0.402HisHis: 0.402 ± 0.022
1.466HisIle: 1.466 ± 0.039
2.069HisLys: 2.069 ± 0.049
1.936HisLeu: 1.936 ± 0.046
0.342HisMet: 0.342 ± 0.019
1.26HisAsn: 1.26 ± 0.038
0.613HisPro: 0.613 ± 0.024
0.473HisGln: 0.473 ± 0.024
0.838HisArg: 0.838 ± 0.034
1.579HisSer: 1.579 ± 0.04
0.889HisThr: 0.889 ± 0.031
0.825HisVal: 0.825 ± 0.03
0.103HisTrp: 0.103 ± 0.011
0.751HisTyr: 0.751 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
2.798IleAla: 2.798 ± 0.054
2.02IleCys: 2.02 ± 0.039
4.965IleAsp: 4.965 ± 0.066
6.602IleGlu: 6.602 ± 0.087
6.93IlePhe: 6.93 ± 0.096
3.196IleGly: 3.196 ± 0.062
1.841IleHis: 1.841 ± 0.043
9.352IleIle: 9.352 ± 0.119
10.867IleLys: 10.867 ± 0.119
10.456IleLeu: 10.456 ± 0.121
1.583IleMet: 1.583 ± 0.049
8.405IleAsn: 8.405 ± 0.112
2.881IlePro: 2.881 ± 0.052
2.603IleGln: 2.603 ± 0.052
3.105IleArg: 3.105 ± 0.058
7.245IleSer: 7.245 ± 0.082
4.094IleThr: 4.094 ± 0.061
4.343IleVal: 4.343 ± 0.061
0.557IleTrp: 0.557 ± 0.022
5.07IleTyr: 5.07 ± 0.083
0.0IleXaa: 0.0 ± 0.0
Lys
2.739LysAla: 2.739 ± 0.061
1.835LysCys: 1.835 ± 0.049
5.307LysAsp: 5.307 ± 0.077
10.639LysGlu: 10.639 ± 0.133
5.05LysPhe: 5.05 ± 0.065
3.319LysGly: 3.319 ± 0.059
1.715LysHis: 1.715 ± 0.043
11.002LysIle: 11.002 ± 0.123
11.584LysLys: 11.584 ± 0.151
8.909LysLeu: 8.909 ± 0.092
2.407LysMet: 2.407 ± 0.047
9.521LysAsn: 9.521 ± 0.112
2.108LysPro: 2.108 ± 0.056
2.476LysGln: 2.476 ± 0.055
4.578LysArg: 4.578 ± 0.065
6.864LysSer: 6.864 ± 0.099
4.501LysThr: 4.501 ± 0.073
4.495LysVal: 4.495 ± 0.067
0.476LysTrp: 0.476 ± 0.024
5.498LysTyr: 5.498 ± 0.075
0.0LysXaa: 0.0 ± 0.0
Leu
2.933LeuAla: 2.933 ± 0.059
1.964LeuCys: 1.964 ± 0.05
4.575LeuAsp: 4.575 ± 0.075
7.105LeuGlu: 7.105 ± 0.094
6.512LeuPhe: 6.512 ± 0.094
3.482LeuGly: 3.482 ± 0.064
1.812LeuHis: 1.812 ± 0.044
10.106LeuIle: 10.106 ± 0.118
11.008LeuLys: 11.008 ± 0.116
10.367LeuLeu: 10.367 ± 0.13
2.149LeuMet: 2.149 ± 0.046
8.063LeuAsn: 8.063 ± 0.09
2.611LeuPro: 2.611 ± 0.054
2.979LeuGln: 2.979 ± 0.062
3.769LeuArg: 3.769 ± 0.058
7.102LeuSer: 7.102 ± 0.087
4.41LeuThr: 4.41 ± 0.059
4.488LeuVal: 4.488 ± 0.068
0.513LeuTrp: 0.513 ± 0.022
4.195LeuTyr: 4.195 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
0.648MetAla: 0.648 ± 0.028
0.318MetCys: 0.318 ± 0.017
1.055MetAsp: 1.055 ± 0.032
1.475MetGlu: 1.475 ± 0.046
1.305MetPhe: 1.305 ± 0.037
0.66MetGly: 0.66 ± 0.026
0.517MetHis: 0.517 ± 0.022
2.155MetIle: 2.155 ± 0.047
2.17MetLys: 2.17 ± 0.047
2.158MetLeu: 2.158 ± 0.051
0.378MetMet: 0.378 ± 0.019
1.925MetAsn: 1.925 ± 0.05
0.568MetPro: 0.568 ± 0.024
0.713MetGln: 0.713 ± 0.028
0.748MetArg: 0.748 ± 0.028
1.343MetSer: 1.343 ± 0.038
0.875MetThr: 0.875 ± 0.03
1.006MetVal: 1.006 ± 0.031
0.125MetTrp: 0.125 ± 0.011
0.779MetTyr: 0.779 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
2.139AsnAla: 2.139 ± 0.043
1.256AsnCys: 1.256 ± 0.036
4.488AsnAsp: 4.488 ± 0.062
6.584AsnGlu: 6.584 ± 0.096
4.85AsnPhe: 4.85 ± 0.075
2.484AsnGly: 2.484 ± 0.08
1.564AsnHis: 1.564 ± 0.039
8.253AsnIle: 8.253 ± 0.11
9.059AsnLys: 9.059 ± 0.102
8.174AsnLeu: 8.174 ± 0.088
1.581AsnMet: 1.581 ± 0.044
6.691AsnAsn: 6.691 ± 0.126
1.857AsnPro: 1.857 ± 0.041
2.188AsnGln: 2.188 ± 0.045
2.45AsnArg: 2.45 ± 0.048
5.389AsnSer: 5.389 ± 0.082
3.83AsnThr: 3.83 ± 0.064
3.424AsnVal: 3.424 ± 0.056
0.404AsnTrp: 0.404 ± 0.018
4.029AsnTyr: 4.029 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
0.708ProAla: 0.708 ± 0.038
0.42ProCys: 0.42 ± 0.02
1.047ProAsp: 1.047 ± 0.032
1.829ProGlu: 1.829 ± 0.047
1.588ProPhe: 1.588 ± 0.039
1.044ProGly: 1.044 ± 0.042
0.449ProHis: 0.449 ± 0.02
2.394ProIle: 2.394 ± 0.046
2.226ProLys: 2.226 ± 0.052
2.345ProLeu: 2.345 ± 0.052
0.572ProMet: 0.572 ± 0.024
1.783ProAsn: 1.783 ± 0.037
0.726ProPro: 0.726 ± 0.057
0.901ProGln: 0.901 ± 0.034
0.719ProArg: 0.719 ± 0.033
1.933ProSer: 1.933 ± 0.044
1.241ProThr: 1.241 ± 0.043
1.151ProVal: 1.151 ± 0.036
0.138ProTrp: 0.138 ± 0.013
1.037ProTyr: 1.037 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
0.837GlnAla: 0.837 ± 0.028
0.41GlnCys: 0.41 ± 0.021
1.051GlnAsp: 1.051 ± 0.035
2.087GlnGlu: 2.087 ± 0.045
1.245GlnPhe: 1.245 ± 0.035
0.857GlnGly: 0.857 ± 0.035
0.421GlnHis: 0.421 ± 0.021
2.586GlnIle: 2.586 ± 0.061
2.726GlnLys: 2.726 ± 0.058
2.377GlnLeu: 2.377 ± 0.051
0.685GlnMet: 0.685 ± 0.027
2.383GlnAsn: 2.383 ± 0.056
0.733GlnPro: 0.733 ± 0.033
0.851GlnGln: 0.851 ± 0.042
1.3GlnArg: 1.3 ± 0.034
1.937GlnSer: 1.937 ± 0.046
1.208GlnThr: 1.208 ± 0.035
1.104GlnVal: 1.104 ± 0.035
0.18GlnTrp: 0.18 ± 0.014
0.899GlnTyr: 0.899 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
1.123ArgAla: 1.123 ± 0.037
0.669ArgCys: 0.669 ± 0.027
1.538ArgAsp: 1.538 ± 0.038
2.706ArgGlu: 2.706 ± 0.06
1.827ArgPhe: 1.827 ± 0.038
1.423ArgGly: 1.423 ± 0.04
0.598ArgHis: 0.598 ± 0.023
3.9ArgIle: 3.9 ± 0.066
4.382ArgLys: 4.382 ± 0.069
2.853ArgLeu: 2.853 ± 0.054
0.96ArgMet: 0.96 ± 0.029
3.302ArgAsn: 3.302 ± 0.062
0.711ArgPro: 0.711 ± 0.03
0.805ArgGln: 0.805 ± 0.03
1.842ArgArg: 1.842 ± 0.05
2.345ArgSer: 2.345 ± 0.053
1.599ArgThr: 1.599 ± 0.039
1.599ArgVal: 1.599 ± 0.037
0.229ArgTrp: 0.229 ± 0.017
1.391ArgTyr: 1.391 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
2.166SerAla: 2.166 ± 0.054
1.266SerCys: 1.266 ± 0.04
3.781SerAsp: 3.781 ± 0.062
4.805SerGlu: 4.805 ± 0.069
5.474SerPhe: 5.474 ± 0.076
2.362SerGly: 2.362 ± 0.064
1.415SerHis: 1.415 ± 0.035
6.814SerIle: 6.814 ± 0.083
7.178SerLys: 7.178 ± 0.085
7.887SerLeu: 7.887 ± 0.101
1.42SerMet: 1.42 ± 0.035
5.164SerAsn: 5.164 ± 0.067
1.609SerPro: 1.609 ± 0.043
1.851SerGln: 1.851 ± 0.046
2.153SerArg: 2.153 ± 0.046
6.058SerSer: 6.058 ± 0.137
3.547SerThr: 3.547 ± 0.06
3.492SerVal: 3.492 ± 0.055
0.358SerTrp: 0.358 ± 0.021
3.312SerTyr: 3.312 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
1.303ThrAla: 1.303 ± 0.036
0.795ThrCys: 0.795 ± 0.028
2.591ThrAsp: 2.591 ± 0.053
3.05ThrGlu: 3.05 ± 0.057
2.862ThrPhe: 2.862 ± 0.048
1.712ThrGly: 1.712 ± 0.046
0.953ThrHis: 0.953 ± 0.032
4.299ThrIle: 4.299 ± 0.079
4.448ThrLys: 4.448 ± 0.06
4.569ThrLeu: 4.569 ± 0.066
0.896ThrMet: 0.896 ± 0.032
3.612ThrAsn: 3.612 ± 0.066
1.404ThrPro: 1.404 ± 0.045
1.48ThrGln: 1.48 ± 0.034
1.513ThrArg: 1.513 ± 0.036
3.292ThrSer: 3.292 ± 0.065
2.299ThrThr: 2.299 ± 0.056
2.222ThrVal: 2.222 ± 0.046
0.269ThrTrp: 0.269 ± 0.017
1.891ThrTyr: 1.891 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
1.519ValAla: 1.519 ± 0.039
0.98ValCys: 0.98 ± 0.032
2.609ValAsp: 2.609 ± 0.057
3.666ValGlu: 3.666 ± 0.071
3.062ValPhe: 3.062 ± 0.054
1.627ValGly: 1.627 ± 0.048
0.839ValHis: 0.839 ± 0.034
4.397ValIle: 4.397 ± 0.058
4.687ValLys: 4.687 ± 0.076
4.642ValLeu: 4.642 ± 0.073
0.893ValMet: 0.893 ± 0.031
3.669ValAsn: 3.669 ± 0.062
1.363ValPro: 1.363 ± 0.04
1.18ValGln: 1.18 ± 0.037
1.527ValArg: 1.527 ± 0.039
3.361ValSer: 3.361 ± 0.054
1.992ValThr: 1.992 ± 0.049
2.492ValVal: 2.492 ± 0.054
0.325ValTrp: 0.325 ± 0.019
2.253ValTyr: 2.253 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.167TrpAla: 0.167 ± 0.014
0.149TrpCys: 0.149 ± 0.014
0.264TrpAsp: 0.264 ± 0.018
0.287TrpGlu: 0.287 ± 0.016
0.334TrpPhe: 0.334 ± 0.019
0.197TrpGly: 0.197 ± 0.016
0.094TrpHis: 0.094 ± 0.009
0.563TrpIle: 0.563 ± 0.023
0.54TrpLys: 0.54 ± 0.023
0.516TrpLeu: 0.516 ± 0.024
0.13TrpMet: 0.13 ± 0.013
0.388TrpAsn: 0.388 ± 0.02
0.104TrpPro: 0.104 ± 0.01
0.148TrpGln: 0.148 ± 0.012
0.357TrpArg: 0.357 ± 0.019
0.462TrpSer: 0.462 ± 0.021
0.29TrpThr: 0.29 ± 0.02
0.355TrpVal: 0.355 ± 0.018
0.037TrpTrp: 0.037 ± 0.007
0.255TrpTyr: 0.255 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.392TyrAla: 1.392 ± 0.034
0.902TyrCys: 0.902 ± 0.03
2.445TyrAsp: 2.445 ± 0.049
3.17TyrGlu: 3.17 ± 0.058
4.34TyrPhe: 4.34 ± 0.071
1.463TyrGly: 1.463 ± 0.04
0.93TyrHis: 0.93 ± 0.029
3.894TyrIle: 3.894 ± 0.063
5.124TyrLys: 5.124 ± 0.079
5.47TyrLeu: 5.47 ± 0.083
0.841TyrMet: 0.841 ± 0.028
3.616TyrAsn: 3.616 ± 0.06
1.206TyrPro: 1.206 ± 0.042
1.217TyrGln: 1.217 ± 0.035
1.324TyrArg: 1.324 ± 0.042
3.611TyrSer: 3.611 ± 0.054
1.929TyrThr: 1.929 ± 0.047
2.018TyrVal: 2.018 ± 0.044
0.277TyrTrp: 0.277 ± 0.016
2.312TyrTyr: 2.312 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5143 proteins (993622 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski