Sheep faeces associated smacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus sheas2

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161J5M0|A0A161J5M0_9VIRU Putative capsid protein OS=Sheep faeces associated smacovirus 2 OX=1843757 PE=4 SV=1
MM1 pKa = 6.4TQSIAVSISEE11 pKa = 4.52TYY13 pKa = 10.73DD14 pKa = 3.7LSTTLNKK21 pKa = 9.78MGLIAIRR28 pKa = 11.84TPGMQSVEE36 pKa = 3.49KK37 pKa = 9.82RR38 pKa = 11.84YY39 pKa = 9.82PGFVRR44 pKa = 11.84NFKK47 pKa = 10.01FLRR50 pKa = 11.84VKK52 pKa = 10.59SCDD55 pKa = 3.58VVISCASSLPADD67 pKa = 4.04PLQIGTAAGSIAPQDD82 pKa = 3.58MFNPLLYY89 pKa = 10.4KK90 pKa = 10.52AVSNDD95 pKa = 2.77SWNGLLNRR103 pKa = 11.84IYY105 pKa = 10.93GSTGAVNSLGGTVRR119 pKa = 11.84FFQDD123 pKa = 2.67AFSAASSADD132 pKa = 3.76SINSYY137 pKa = 10.56YY138 pKa = 11.28ALLSDD143 pKa = 4.12PSFKK147 pKa = 10.2KK148 pKa = 10.55AHH150 pKa = 5.01VQQGLEE156 pKa = 3.94MRR158 pKa = 11.84RR159 pKa = 11.84LVPLVYY165 pKa = 10.16HH166 pKa = 6.16VLNAGGTTPNMNGATLANVDD186 pKa = 3.85EE187 pKa = 5.05LDD189 pKa = 3.68NVNAVGNNGVSVSASNNMLFGGTAFFKK216 pKa = 10.6GRR218 pKa = 11.84PVPMPPVEE226 pKa = 4.28CTTSVVHH233 pKa = 5.91PTDD236 pKa = 3.24GTVTQTVFNPIIGNIPSTYY255 pKa = 9.35VAAIVTPPATLNITYY270 pKa = 10.0FRR272 pKa = 11.84MTVRR276 pKa = 11.84WNLEE280 pKa = 3.92FFGVASDD287 pKa = 3.34IAKK290 pKa = 10.73ALMPGTSDD298 pKa = 2.64IGRR301 pKa = 11.84YY302 pKa = 9.58AYY304 pKa = 10.3FSSNVSQQTLSASKK318 pKa = 10.32ISDD321 pKa = 3.48VSEE324 pKa = 4.08TAVNADD330 pKa = 3.93DD331 pKa = 4.63QEE333 pKa = 5.72HH334 pKa = 7.22DD335 pKa = 3.22IQRR338 pKa = 11.84TVEE341 pKa = 3.99ADD343 pKa = 3.45GVTLDD348 pKa = 5.77LIMEE352 pKa = 4.61KK353 pKa = 10.72

Molecular weight: 37.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161J5M0|A0A161J5M0_9VIRU Putative capsid protein OS=Sheep faeces associated smacovirus 2 OX=1843757 PE=4 SV=1
MM1 pKa = 6.36MTIPRR6 pKa = 11.84RR7 pKa = 11.84IEE9 pKa = 3.83KK10 pKa = 10.16PLDD13 pKa = 3.18NWCWQCEE20 pKa = 4.26RR21 pKa = 11.84ICSKK25 pKa = 10.99KK26 pKa = 9.26LLSYY30 pKa = 10.43IFEE33 pKa = 4.09QADD36 pKa = 3.36VKK38 pKa = 10.82RR39 pKa = 11.84YY40 pKa = 9.3IVAIEE45 pKa = 3.93KK46 pKa = 10.4GKK48 pKa = 10.6NGLDD52 pKa = 3.12HH53 pKa = 6.32FQIRR57 pKa = 11.84LSCSDD62 pKa = 3.61PDD64 pKa = 3.93FFEE67 pKa = 5.83HH68 pKa = 6.38MKK70 pKa = 10.5DD71 pKa = 2.94WCEE74 pKa = 3.53WAHH77 pKa = 5.31VEE79 pKa = 5.47KK80 pKa = 11.24AEE82 pKa = 4.15TDD84 pKa = 3.25NFDD87 pKa = 3.59YY88 pKa = 10.67EE89 pKa = 4.35RR90 pKa = 11.84KK91 pKa = 9.84EE92 pKa = 3.96GRR94 pKa = 11.84FWTSDD99 pKa = 3.19DD100 pKa = 3.4TTEE103 pKa = 4.22IRR105 pKa = 11.84ICRR108 pKa = 11.84FRR110 pKa = 11.84EE111 pKa = 3.64LGGPGRR117 pKa = 11.84QWQKK121 pKa = 11.27QIMQVLKK128 pKa = 10.05KK129 pKa = 10.27QDD131 pKa = 3.05VRR133 pKa = 11.84TIDD136 pKa = 3.48VVLDD140 pKa = 3.45PVGARR145 pKa = 11.84GKK147 pKa = 9.52SHH149 pKa = 7.2FAIALWEE156 pKa = 4.24RR157 pKa = 11.84GEE159 pKa = 3.99ALVVPRR165 pKa = 11.84YY166 pKa = 10.36SCTAEE171 pKa = 3.94KK172 pKa = 10.67LSAFVCSAYY181 pKa = 10.18RR182 pKa = 11.84GRR184 pKa = 11.84KK185 pKa = 8.88IIIIDD190 pKa = 3.42IPRR193 pKa = 11.84ANKK196 pKa = 7.67PTTALYY202 pKa = 8.45EE203 pKa = 4.29TMEE206 pKa = 4.06EE207 pKa = 4.21MKK209 pKa = 10.64DD210 pKa = 3.46GLVFDD215 pKa = 4.86PRR217 pKa = 11.84YY218 pKa = 9.88SGKK221 pKa = 8.15TRR223 pKa = 11.84NIRR226 pKa = 11.84GTKK229 pKa = 10.13VLVFTNNPLDD239 pKa = 4.06LKK241 pKa = 10.85KK242 pKa = 10.67LSHH245 pKa = 7.11DD246 pKa = 2.93RR247 pKa = 11.84WNLHH251 pKa = 6.32GISVDD256 pKa = 3.46GTLTT260 pKa = 3.23

Molecular weight: 30.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

613

260

353

306.5

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.341 ± 1.425

1.794 ± 0.781

6.199 ± 0.675

4.568 ± 1.434

4.405 ± 0.106

6.525 ± 0.46

1.631 ± 0.412

6.199 ± 0.675

5.22 ± 1.739

7.341 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.283

5.22 ± 1.305

4.731 ± 0.539

3.263 ± 0.113

5.71 ± 1.675

7.667 ± 1.858

7.015 ± 0.758

7.83 ± 1.255

1.631 ± 0.88

2.936 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski