Corynebacterium phage TouchMeNot

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Poushouvirus; unclassified Poushouvirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QAD8|A0A2Z4QAD8_9CAUD Head-to-tail connector complex protein OS=Corynebacterium phage TouchMeNot OX=2201425 GN=10 PE=4 SV=1
MM1 pKa = 7.09YY2 pKa = 10.33SSSEE6 pKa = 3.97SLRR9 pKa = 11.84EE10 pKa = 3.9HH11 pKa = 7.01EE12 pKa = 4.74SYY14 pKa = 11.54EE15 pKa = 4.39DD16 pKa = 3.5INVLGMIGLALFAIAIFFALIQISVLAGVGASLL49 pKa = 4.61

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q9G7|A0A2Z4Q9G7_9CAUD Uncharacterized protein OS=Corynebacterium phage TouchMeNot OX=2201425 GN=11 PE=4 SV=1
MM1 pKa = 7.45YY2 pKa = 10.01GRR4 pKa = 11.84CEE6 pKa = 3.78QCGEE10 pKa = 4.11RR11 pKa = 11.84VEE13 pKa = 4.61MKK15 pKa = 10.73ASGPSARR22 pKa = 11.84YY23 pKa = 9.08CSARR27 pKa = 11.84CRR29 pKa = 11.84QRR31 pKa = 11.84AYY33 pKa = 10.52RR34 pKa = 11.84ARR36 pKa = 11.84CKK38 pKa = 10.31KK39 pKa = 10.03QLPARR44 pKa = 11.84MRR46 pKa = 11.84EE47 pKa = 4.09LPRR50 pKa = 11.84WTAAAGKK57 pKa = 10.31RR58 pKa = 11.84PVTTDD63 pKa = 3.54GAPASSTDD71 pKa = 3.21AQTWTSHH78 pKa = 5.28EE79 pKa = 4.22EE80 pKa = 4.5VVGGPHH86 pKa = 6.58GVMLGGGLACIDD98 pKa = 4.69LDD100 pKa = 3.64HH101 pKa = 6.95CLSARR106 pKa = 11.84GKK108 pKa = 8.61LAPWARR114 pKa = 11.84EE115 pKa = 3.86IIDD118 pKa = 3.98AVPGAVVEE126 pKa = 4.29RR127 pKa = 11.84SVSKK131 pKa = 10.71RR132 pKa = 11.84GIHH135 pKa = 6.29IFGLLPEE142 pKa = 4.63APGRR146 pKa = 11.84RR147 pKa = 11.84RR148 pKa = 11.84GRR150 pKa = 11.84AEE152 pKa = 3.74VYY154 pKa = 10.11SRR156 pKa = 11.84ARR158 pKa = 11.84FIRR161 pKa = 11.84TTEE164 pKa = 4.28DD165 pKa = 2.66IYY167 pKa = 11.29RR168 pKa = 11.84VGKK171 pKa = 10.44IIDD174 pKa = 3.87LQPAVTKK181 pKa = 10.8LNSLEE186 pKa = 4.0RR187 pKa = 11.84RR188 pKa = 11.84GEE190 pKa = 3.91IPTT193 pKa = 3.79

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12819

44

1635

237.4

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.842 ± 0.627

0.725 ± 0.158

6.327 ± 0.229

6.818 ± 0.39

2.871 ± 0.182

7.832 ± 0.472

1.981 ± 0.17

5.461 ± 0.16

4.353 ± 0.368

8.004 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.145

2.723 ± 0.222

4.899 ± 0.27

4.189 ± 0.33

6.444 ± 0.468

6.717 ± 0.362

5.687 ± 0.336

6.67 ± 0.243

1.849 ± 0.213

2.239 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski