Erythrobacter insulae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2737 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A547PCW0|A0A547PCW0_9SPHN Acyl-CoA dehydrogenase OS=Erythrobacter insulae OX=2584124 GN=FGU71_08465 PE=3 SV=1
MM1 pKa = 7.11QRR3 pKa = 11.84MSHH6 pKa = 6.44ISWNSIGRR14 pKa = 11.84VGKK17 pKa = 7.63TAAMAAALGATSVCFADD34 pKa = 4.03EE35 pKa = 4.19AAAQTTTFTTQSVPDD50 pKa = 3.43TWTAVYY56 pKa = 10.83ADD58 pKa = 3.64ADD60 pKa = 3.67GRR62 pKa = 11.84TITVTMQPRR71 pKa = 11.84DD72 pKa = 3.77GSCAAINALGTIGSYY87 pKa = 8.85ATSSVAACDD96 pKa = 3.06RR97 pKa = 11.84AIRR100 pKa = 11.84ITYY103 pKa = 6.44VTSGFLVQEE112 pKa = 3.79IRR114 pKa = 11.84INDD117 pKa = 3.79IDD119 pKa = 5.26DD120 pKa = 3.61MDD122 pKa = 4.06GTGPRR127 pKa = 11.84DD128 pKa = 3.54GVAMTVPGTWSSSTIEE144 pKa = 4.06VHH146 pKa = 6.89SFAAPPLFANQAARR160 pKa = 11.84LTAAGGVGTFIANAAGDD177 pKa = 3.95NPVNEE182 pKa = 4.35AATFTMAAPTNTFTMLHH199 pKa = 7.31DD200 pKa = 4.54DD201 pKa = 3.93VQDD204 pKa = 3.74GRR206 pKa = 11.84DD207 pKa = 3.23ALFNFDD213 pKa = 4.72LNGMTITTAEE223 pKa = 4.2GNILTVDD230 pKa = 3.75DD231 pKa = 4.66SGSIASTTGGTNVLNVYY248 pKa = 10.64DD249 pKa = 4.87GDD251 pKa = 4.11TLNGVAAAPINTDD264 pKa = 2.71VSLVSGTTLQQGITFDD280 pKa = 3.52AATGEE285 pKa = 4.35VSVAAGTPVGEE296 pKa = 3.98YY297 pKa = 10.5SFDD300 pKa = 3.79YY301 pKa = 10.01EE302 pKa = 3.82ICEE305 pKa = 3.96VGFAANCEE313 pKa = 4.31TGTATVTVTGNVDD326 pKa = 3.85LDD328 pKa = 3.48ITKK331 pKa = 10.13TNTPGVNGEE340 pKa = 4.0IDD342 pKa = 3.34QATDD346 pKa = 3.13TVLAGSNTTYY356 pKa = 10.65TVIVTNNGPNPVNGAVVSDD375 pKa = 4.05TPGSGITCDD384 pKa = 3.23GATPITLTGSGVPAGSYY401 pKa = 9.19TFADD405 pKa = 3.17ISGVGISLEE414 pKa = 4.21TLSTGQSTTLSYY426 pKa = 11.02SCQVNN431 pKa = 3.11

Molecular weight: 43.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A547PCG5|A0A547PCG5_9SPHN Beta-galactosidase OS=Erythrobacter insulae OX=2584124 GN=FGU71_08070 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 9.21VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.98RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.08SLSAA44 pKa = 3.93

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2737

0

2737

904613

30

4491

330.5

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.173 ± 0.062

0.792 ± 0.016

6.259 ± 0.039

6.203 ± 0.046

3.843 ± 0.038

8.747 ± 0.049

1.873 ± 0.024

5.583 ± 0.035

3.503 ± 0.04

9.427 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.026

2.993 ± 0.033

4.976 ± 0.031

3.284 ± 0.025

6.361 ± 0.044

5.793 ± 0.036

5.319 ± 0.033

6.747 ± 0.033

1.379 ± 0.02

2.198 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski