Fischerella thermalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Hapalosiphonaceae; Fischerella

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4557 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G6FWV6|G6FWV6_9CYAN PapD_N domain-containing protein OS=Fischerella thermalis OX=372787 GN=FJSC11DRAFT_3355 PE=4 SV=1
MM1 pKa = 6.94TVRR4 pKa = 11.84VHH6 pKa = 6.89FLPDD10 pKa = 3.72DD11 pKa = 3.87VTVDD15 pKa = 3.59AQVGEE20 pKa = 4.33PLLDD24 pKa = 3.37VADD27 pKa = 4.33RR28 pKa = 11.84AEE30 pKa = 4.26VIIPTGCLMGTCHH43 pKa = 7.06ACTVEE48 pKa = 4.51LDD50 pKa = 3.48DD51 pKa = 5.46GEE53 pKa = 4.71IIRR56 pKa = 11.84ACISSVPDD64 pKa = 3.29GCEE67 pKa = 3.39EE68 pKa = 3.79LTINLFSDD76 pKa = 4.03PTWW79 pKa = 4.29

Molecular weight:
8.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G6FNJ6|G6FNJ6_9CYAN TonB-dependent receptor OS=Fischerella thermalis OX=372787 GN=FJSC11DRAFT_0443 PE=3 SV=1
MM1 pKa = 6.99SQSSSKK7 pKa = 10.71LNRR10 pKa = 11.84RR11 pKa = 11.84LTQAVGIVLGITIAVWVLRR30 pKa = 11.84GFGVLTFLPGGIILMLLLVAIAIGILSHH58 pKa = 6.6LQKK61 pKa = 9.66TWWRR65 pKa = 11.84FF66 pKa = 3.05

Molecular weight:
7.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4557

0

4557

1410942

30

2376

309.6

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.223 ± 0.044

1.02 ± 0.012

4.674 ± 0.029

6.13 ± 0.043

3.934 ± 0.027

6.606 ± 0.034

1.895 ± 0.021

6.788 ± 0.028

4.854 ± 0.031

10.976 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.906 ± 0.015

4.296 ± 0.03

4.81 ± 0.03

5.505 ± 0.034

5.162 ± 0.034

6.142 ± 0.03

5.736 ± 0.029

6.866 ± 0.026

1.422 ± 0.018

3.053 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski