Hubei tombus-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGJ3|A0A1L3KGJ3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 10 OX=1923256 PE=4 SV=1
MM1 pKa = 8.19PDD3 pKa = 3.44LDD5 pKa = 3.72LGIRR9 pKa = 11.84VRR11 pKa = 11.84SFGSPIRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84STYY24 pKa = 8.58VSMVAGRR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.69VFVHH37 pKa = 6.12NSSVHH42 pKa = 5.31NALLAAKK49 pKa = 9.75IRR51 pKa = 11.84VLTAEE56 pKa = 4.15VDD58 pKa = 3.77GKK60 pKa = 10.63LVPIEE65 pKa = 4.18EE66 pKa = 4.41TRR68 pKa = 11.84QPTGHH73 pKa = 5.62SHH75 pKa = 6.73ILDD78 pKa = 3.86GVLTTMQRR86 pKa = 11.84DD87 pKa = 3.93LPILTPVPLEE97 pKa = 4.02QFPGLYY103 pKa = 9.86KK104 pKa = 10.51AEE106 pKa = 4.05KK107 pKa = 9.26KK108 pKa = 10.37KK109 pKa = 10.37IYY111 pKa = 9.78EE112 pKa = 4.15RR113 pKa = 11.84AVSSLSVCPLTSDD126 pKa = 3.7DD127 pKa = 3.8SKK129 pKa = 11.14IRR131 pKa = 11.84AFIKK135 pKa = 10.12FEE137 pKa = 3.91KK138 pKa = 9.94MLAKK142 pKa = 9.43EE143 pKa = 4.12TGKK146 pKa = 9.95PKK148 pKa = 10.14APRR151 pKa = 11.84MISPPSPRR159 pKa = 11.84FLVRR163 pKa = 11.84TGCYY167 pKa = 9.46VKK169 pKa = 10.21PAEE172 pKa = 4.09HH173 pKa = 7.18AIYY176 pKa = 10.08EE177 pKa = 4.85AIDD180 pKa = 3.09HH181 pKa = 5.82MFGFKK186 pKa = 10.42VVTKK190 pKa = 10.24GMNFGEE196 pKa = 4.25IGRR199 pKa = 11.84LFKK202 pKa = 10.72SHH204 pKa = 6.39WDD206 pKa = 3.54ALDD209 pKa = 4.15DD210 pKa = 4.03PVAFDD215 pKa = 3.78VDD217 pKa = 4.0VEE219 pKa = 4.25KK220 pKa = 10.31MDD222 pKa = 4.1RR223 pKa = 11.84STSSEE228 pKa = 3.83MLAWTHH234 pKa = 6.39KK235 pKa = 10.4LIHH238 pKa = 6.0ACYY241 pKa = 10.13RR242 pKa = 11.84GDD244 pKa = 3.89DD245 pKa = 3.78LDD247 pKa = 6.15DD248 pKa = 3.47IRR250 pKa = 11.84EE251 pKa = 4.04MLKK254 pKa = 9.65QQLSVRR260 pKa = 11.84TTVKK264 pKa = 10.67CDD266 pKa = 3.5DD267 pKa = 3.94GNIKK271 pKa = 9.13YY272 pKa = 8.24TVDD275 pKa = 3.15GTLTSGQMNTSLVGVSMVSSIMYY298 pKa = 8.77TLFKK302 pKa = 10.69EE303 pKa = 4.36RR304 pKa = 11.84LDD306 pKa = 3.57VPYY309 pKa = 10.6RR310 pKa = 11.84FVDD313 pKa = 3.48AGDD316 pKa = 4.13DD317 pKa = 3.75CTVILDD323 pKa = 3.9KK324 pKa = 11.33RR325 pKa = 11.84NADD328 pKa = 3.56RR329 pKa = 11.84FLCEE333 pKa = 3.39VRR335 pKa = 11.84KK336 pKa = 10.17IFGEE340 pKa = 4.05VGFAITIGPPSVEE353 pKa = 3.81LEE355 pKa = 4.0QIEE358 pKa = 4.87FCQGHH363 pKa = 6.34PVLVGSSYY371 pKa = 11.06TMVRR375 pKa = 11.84NAKK378 pKa = 9.88DD379 pKa = 3.29AAIKK383 pKa = 10.46DD384 pKa = 3.93ATSLQPMDD392 pKa = 4.57SLRR395 pKa = 11.84EE396 pKa = 3.68MAVWMEE402 pKa = 3.98AVAKK406 pKa = 10.51CGIASHH412 pKa = 6.49GGVPIASSLYY422 pKa = 8.96RR423 pKa = 11.84CYY425 pKa = 11.14ARR427 pKa = 11.84NSTRR431 pKa = 11.84MQRR434 pKa = 11.84EE435 pKa = 3.61MKK437 pKa = 7.99MTQRR441 pKa = 11.84QLKK444 pKa = 9.04RR445 pKa = 11.84FKK447 pKa = 10.83LAVDD451 pKa = 3.38KK452 pKa = 10.95RR453 pKa = 11.84KK454 pKa = 10.29KK455 pKa = 10.62DD456 pKa = 3.59VVSWNQSNAEE466 pKa = 4.11PMCALDD472 pKa = 4.74VEE474 pKa = 4.66PSPYY478 pKa = 9.92TRR480 pKa = 11.84LSYY483 pKa = 10.99EE484 pKa = 3.79KK485 pKa = 10.82AFGLNSVFQLRR496 pKa = 11.84MEE498 pKa = 4.17QYY500 pKa = 11.27YY501 pKa = 8.56DD502 pKa = 3.32TLVINWSRR510 pKa = 11.84PVSQAQTTYY519 pKa = 10.17NPLWSTVV526 pKa = 3.01

Molecular weight: 59.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGJ3|A0A1L3KGJ3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 10 OX=1923256 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84KK3 pKa = 9.38PSNLKK8 pKa = 9.97SANAWAKK15 pKa = 10.55SLSNPFGAPAAHH27 pKa = 6.7IPDD30 pKa = 4.54FEE32 pKa = 4.93TNSSGMITSTLYY44 pKa = 9.91TQVLPLGYY52 pKa = 10.45ASTATTHH59 pKa = 5.53NVGMIIQPHH68 pKa = 5.96PRR70 pKa = 11.84VHH72 pKa = 6.19FTQLSEE78 pKa = 4.49VNTGTANMTDD88 pKa = 3.66MNSGNTDD95 pKa = 2.71VGALLSVPNLAGFGDD110 pKa = 3.78QVRR113 pKa = 11.84YY114 pKa = 10.56RR115 pKa = 11.84MTSCGVRR122 pKa = 11.84VTYY125 pKa = 10.67AGTEE129 pKa = 4.07LNRR132 pKa = 11.84SGEE135 pKa = 4.25YY136 pKa = 9.8IAGFLQTDD144 pKa = 4.24YY145 pKa = 10.35PAQGTTAAATGIGPMSTLMPKK166 pKa = 8.01TTLGYY171 pKa = 8.2WTMAQIIGSLKK182 pKa = 10.25NPIEE186 pKa = 4.2ARR188 pKa = 11.84ICDD191 pKa = 3.94GTAEE195 pKa = 4.24FHH197 pKa = 6.33WKK199 pKa = 9.58PNGVPGYY206 pKa = 10.44GSGGANIAYY215 pKa = 9.43FPTTSGTAGAVTNPSGYY232 pKa = 10.71ASNPGEE238 pKa = 4.11GGVPYY243 pKa = 10.51GSEE246 pKa = 3.69NLVILIRR253 pKa = 11.84GDD255 pKa = 3.38TTAAAATTGNVYY267 pKa = 10.74DD268 pKa = 3.95VTVTSHH274 pKa = 6.1WEE276 pKa = 4.05VIPSVLNAVVYY287 pKa = 10.27DD288 pKa = 3.95VSPSLSDD295 pKa = 3.65PQALSAAMNVISRR308 pKa = 11.84RR309 pKa = 11.84PNPLFSTHH317 pKa = 5.95GVQEE321 pKa = 4.44TTFEE325 pKa = 4.11EE326 pKa = 3.97PVYY329 pKa = 10.46NVKK332 pKa = 10.36KK333 pKa = 10.82NKK335 pKa = 9.39VKK337 pKa = 10.72QIAQAAQEE345 pKa = 4.18SGLVQEE351 pKa = 5.07LGMRR355 pKa = 11.84ALRR358 pKa = 11.84VAAGIATRR366 pKa = 11.84KK367 pKa = 9.08LAGRR371 pKa = 11.84TRR373 pKa = 11.84APVPPGRR380 pKa = 11.84RR381 pKa = 11.84LEE383 pKa = 4.12FF384 pKa = 4.55

Molecular weight: 40.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

910

384

526

455.0

49.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.462 ± 1.53

1.319 ± 0.493

4.945 ± 1.447

4.615 ± 0.438

3.297 ± 0.428

7.363 ± 1.727

1.978 ± 0.096

4.835 ± 0.252

4.945 ± 1.286

7.363 ± 0.527

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.626 ± 0.471

3.956 ± 1.257

5.934 ± 0.678

3.297 ± 0.055

5.934 ± 0.931

7.692 ± 0.087

7.692 ± 1.523

8.242 ± 0.426

1.099 ± 0.035

3.407 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski