Botryosphaeria dothidea virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6TMZ8|A0A0F6TMZ8_9VIRU Capsid protein OS=Botryosphaeria dothidea virus 1 OX=1516075 GN=CP PE=4 SV=1
MM1 pKa = 7.86AFPMSYY7 pKa = 10.07RR8 pKa = 11.84QFIDD12 pKa = 4.24TIPDD16 pKa = 3.63EE17 pKa = 4.5IFFAYY22 pKa = 10.3AEE24 pKa = 4.46AFAQADD30 pKa = 4.07DD31 pKa = 4.3PVGLLQRR38 pKa = 11.84IRR40 pKa = 11.84EE41 pKa = 4.07ADD43 pKa = 3.5GEE45 pKa = 4.36DD46 pKa = 3.21IEE48 pKa = 5.7FEE50 pKa = 3.89ISGQLVSMPCLIDD63 pKa = 4.37CSDD66 pKa = 4.16ILDD69 pKa = 4.26GTAGTQGPSGATQEE83 pKa = 3.9IVEE86 pKa = 4.3AVEE89 pKa = 3.88EE90 pKa = 4.48HH91 pKa = 5.29VTPDD95 pKa = 2.99VGFVGTGVCAQLTIATQRR113 pKa = 11.84LYY115 pKa = 10.39PHH117 pKa = 6.65NEE119 pKa = 3.55IEE121 pKa = 4.39AVRR124 pKa = 11.84AMRR127 pKa = 11.84MDD129 pKa = 5.09DD130 pKa = 3.96GPVDD134 pKa = 3.52TSQPYY139 pKa = 9.82RR140 pKa = 11.84GVAEE144 pKa = 4.25FTGATSDD151 pKa = 3.8PHH153 pKa = 6.48PGWGGDD159 pKa = 3.9ALPAAPVFYY168 pKa = 9.74TGHH171 pKa = 6.5YY172 pKa = 9.97GPAPTCAYY180 pKa = 10.36VGDD183 pKa = 4.07GLVVFAQTHH192 pKa = 4.25THH194 pKa = 6.1LKK196 pKa = 8.41EE197 pKa = 3.99QVRR200 pKa = 11.84TAWTKK205 pKa = 10.52GPRR208 pKa = 11.84EE209 pKa = 4.08GDD211 pKa = 3.44GVSYY215 pKa = 10.49YY216 pKa = 10.88QLAKK220 pKa = 9.6TPHH223 pKa = 5.54GVKK226 pKa = 10.2AFKK229 pKa = 10.28LQYY232 pKa = 8.57VANRR236 pKa = 11.84GTLLWNGRR244 pKa = 11.84VPLGYY249 pKa = 10.42LDD251 pKa = 4.24FVKK254 pKa = 10.64QLGVIRR260 pKa = 11.84ARR262 pKa = 11.84DD263 pKa = 3.98RR264 pKa = 11.84RR265 pKa = 11.84WGPPPGSDD273 pKa = 3.76HH274 pKa = 7.09RR275 pKa = 11.84LEE277 pKa = 4.36PVV279 pKa = 2.74

Molecular weight: 30.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6TN89|A0A0F6TN89_9VIRU Uncharacterized protein OS=Botryosphaeria dothidea virus 1 OX=1516075 PE=4 SV=1
MM1 pKa = 7.99ASTTTTNNGPLLLTKK16 pKa = 10.26EE17 pKa = 4.2QASLLAALGEE27 pKa = 4.51GEE29 pKa = 4.05VAAIIKK35 pKa = 9.52LASLGLRR42 pKa = 11.84PTGILEE48 pKa = 3.84YY49 pKa = 10.88ARR51 pKa = 11.84AVDD54 pKa = 3.9GDD56 pKa = 4.43EE57 pKa = 4.79VPAPPTPNTAAKK69 pKa = 9.68PVTIVAWSFLKK80 pKa = 10.85DD81 pKa = 2.94RR82 pKa = 11.84GQYY85 pKa = 8.81HH86 pKa = 6.05ATYY89 pKa = 10.24GLSAPRR95 pKa = 11.84AGEE98 pKa = 3.97LLEE101 pKa = 5.6LLLTDD106 pKa = 4.17ADD108 pKa = 3.59AAARR112 pKa = 11.84EE113 pKa = 4.1IHH115 pKa = 6.68TIVADD120 pKa = 3.83ALRR123 pKa = 11.84QRR125 pKa = 11.84GSPRR129 pKa = 11.84PVHH132 pKa = 5.24VTLDD136 pKa = 3.76GVPSRR141 pKa = 11.84KK142 pKa = 9.44KK143 pKa = 10.63AGGEE147 pKa = 3.93GAPTGDD153 pKa = 3.77GGLGLLSQEE162 pKa = 4.22IASNPGTYY170 pKa = 10.08GSYY173 pKa = 10.37RR174 pKa = 11.84FVPEE178 pKa = 3.62QTGQPGARR186 pKa = 11.84AYY188 pKa = 10.01RR189 pKa = 11.84VRR191 pKa = 11.84LGGGLYY197 pKa = 10.54VFAQSKK203 pKa = 10.25KK204 pKa = 10.03LATAAARR211 pKa = 11.84ITRR214 pKa = 11.84VKK216 pKa = 10.5GRR218 pKa = 11.84SDD220 pKa = 3.27PLVRR224 pKa = 11.84DD225 pKa = 5.55FVAFWATGSSAKK237 pKa = 10.38FGGEE241 pKa = 3.67IPDD244 pKa = 3.41KK245 pKa = 10.4VTFDD249 pKa = 3.52GRR251 pKa = 11.84QGPNEE256 pKa = 4.22PLQSDD261 pKa = 4.12PTTKK265 pKa = 10.75AKK267 pKa = 7.46TTAGPSSEE275 pKa = 4.04

Molecular weight: 28.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2577

275

757

515.4

55.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.418 ± 0.437

0.97 ± 0.264

6.364 ± 0.329

5.2 ± 0.138

3.221 ± 0.242

7.916 ± 0.695

2.445 ± 0.188

3.454 ± 0.206

2.716 ± 0.452

9.313 ± 0.627

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.306

2.212 ± 0.158

6.17 ± 0.476

2.872 ± 0.294

7.683 ± 0.323

6.558 ± 0.582

6.403 ± 0.467

8.071 ± 0.805

0.854 ± 0.116

2.833 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski