Amino acid dipepetide frequency for Tomitella sp. HY188

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.774AlaAla: 22.774 ± 0.258
1.037AlaCys: 1.037 ± 0.029
9.647AlaAsp: 9.647 ± 0.102
8.442AlaGlu: 8.442 ± 0.11
3.495AlaPhe: 3.495 ± 0.056
14.635AlaGly: 14.635 ± 0.133
2.761AlaHis: 2.761 ± 0.056
4.502AlaIle: 4.502 ± 0.063
2.512AlaLys: 2.512 ± 0.067
12.987AlaLeu: 12.987 ± 0.135
3.119AlaMet: 3.119 ± 0.059
1.876AlaAsn: 1.876 ± 0.042
7.286AlaPro: 7.286 ± 0.104
4.157AlaGln: 4.157 ± 0.071
10.342AlaArg: 10.342 ± 0.135
6.061AlaSer: 6.061 ± 0.076
6.822AlaThr: 6.822 ± 0.073
12.765AlaVal: 12.765 ± 0.132
1.676AlaTrp: 1.676 ± 0.04
2.262AlaTyr: 2.262 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.127CysAla: 1.127 ± 0.035
0.094CysCys: 0.094 ± 0.011
0.455CysAsp: 0.455 ± 0.02
0.378CysGlu: 0.378 ± 0.02
0.211CysPhe: 0.211 ± 0.015
0.975CysGly: 0.975 ± 0.025
0.164CysHis: 0.164 ± 0.012
0.27CysIle: 0.27 ± 0.016
0.124CysLys: 0.124 ± 0.01
0.561CysLeu: 0.561 ± 0.022
0.126CysMet: 0.126 ± 0.011
0.146CysAsn: 0.146 ± 0.013
0.488CysPro: 0.488 ± 0.022
0.161CysGln: 0.161 ± 0.011
0.569CysArg: 0.569 ± 0.023
0.483CysSer: 0.483 ± 0.022
0.513CysThr: 0.513 ± 0.021
0.59CysVal: 0.59 ± 0.022
0.105CysTrp: 0.105 ± 0.01
0.16CysTyr: 0.16 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.684AspAla: 9.684 ± 0.101
0.419AspCys: 0.419 ± 0.019
4.543AspAsp: 4.543 ± 0.068
4.365AspGlu: 4.365 ± 0.064
1.649AspPhe: 1.649 ± 0.036
7.611AspGly: 7.611 ± 0.083
1.394AspHis: 1.394 ± 0.037
2.122AspIle: 2.122 ± 0.046
1.202AspLys: 1.202 ± 0.035
5.908AspLeu: 5.908 ± 0.073
1.042AspMet: 1.042 ± 0.028
0.963AspAsn: 0.963 ± 0.029
4.814AspPro: 4.814 ± 0.074
1.515AspGln: 1.515 ± 0.037
4.843AspArg: 4.843 ± 0.077
3.085AspSer: 3.085 ± 0.055
3.303AspThr: 3.303 ± 0.057
5.374AspVal: 5.374 ± 0.075
0.877AspTrp: 0.877 ± 0.028
1.273AspTyr: 1.273 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.472GluAla: 6.472 ± 0.094
0.38GluCys: 0.38 ± 0.019
2.964GluAsp: 2.964 ± 0.053
2.713GluGlu: 2.713 ± 0.05
1.762GluPhe: 1.762 ± 0.041
4.022GluGly: 4.022 ± 0.061
1.617GluHis: 1.617 ± 0.039
2.345GluIle: 2.345 ± 0.048
1.186GluLys: 1.186 ± 0.035
5.992GluLeu: 5.992 ± 0.086
1.183GluMet: 1.183 ± 0.038
1.079GluAsn: 1.079 ± 0.031
2.96GluPro: 2.96 ± 0.048
2.293GluGln: 2.293 ± 0.046
4.826GluArg: 4.826 ± 0.078
3.07GluSer: 3.07 ± 0.054
2.565GluThr: 2.565 ± 0.045
4.461GluVal: 4.461 ± 0.062
0.856GluTrp: 0.856 ± 0.029
1.205GluTyr: 1.205 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.898PheAla: 3.898 ± 0.066
0.261PheCys: 0.261 ± 0.016
2.25PheAsp: 2.25 ± 0.046
1.445PheGlu: 1.445 ± 0.034
0.896PhePhe: 0.896 ± 0.032
3.285PheGly: 3.285 ± 0.059
0.621PheHis: 0.621 ± 0.022
1.01PheIle: 1.01 ± 0.032
0.422PheLys: 0.422 ± 0.021
2.416PheLeu: 2.416 ± 0.056
0.442PheMet: 0.442 ± 0.02
0.562PheAsn: 0.562 ± 0.025
1.362PhePro: 1.362 ± 0.034
0.569PheGln: 0.569 ± 0.026
1.8PheArg: 1.8 ± 0.043
1.609PheSer: 1.609 ± 0.041
2.019PheThr: 2.019 ± 0.038
2.373PheVal: 2.373 ± 0.046
0.35PheTrp: 0.35 ± 0.017
0.603PheTyr: 0.603 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
12.907GlyAla: 12.907 ± 0.121
0.816GlyCys: 0.816 ± 0.031
6.058GlyAsp: 6.058 ± 0.089
5.058GlyGlu: 5.058 ± 0.067
3.118GlyPhe: 3.118 ± 0.047
10.181GlyGly: 10.181 ± 0.116
2.168GlyHis: 2.168 ± 0.044
4.265GlyIle: 4.265 ± 0.062
2.207GlyLys: 2.207 ± 0.048
8.733GlyLeu: 8.733 ± 0.096
2.51GlyMet: 2.51 ± 0.051
1.758GlyAsn: 1.758 ± 0.04
5.199GlyPro: 5.199 ± 0.081
2.461GlyGln: 2.461 ± 0.053
7.89GlyArg: 7.89 ± 0.085
5.728GlySer: 5.728 ± 0.114
6.223GlyThr: 6.223 ± 0.091
8.137GlyVal: 8.137 ± 0.088
1.566GlyTrp: 1.566 ± 0.039
2.341GlyTyr: 2.341 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.72HisAla: 2.72 ± 0.055
0.214HisCys: 0.214 ± 0.013
1.48HisAsp: 1.48 ± 0.033
1.034HisGlu: 1.034 ± 0.032
0.633HisPhe: 0.633 ± 0.024
2.363HisGly: 2.363 ± 0.042
0.592HisHis: 0.592 ± 0.026
0.712HisIle: 0.712 ± 0.025
0.325HisLys: 0.325 ± 0.017
2.085HisLeu: 2.085 ± 0.048
0.391HisMet: 0.391 ± 0.019
0.371HisAsn: 0.371 ± 0.019
1.668HisPro: 1.668 ± 0.038
0.543HisGln: 0.543 ± 0.021
1.972HisArg: 1.972 ± 0.042
1.081HisSer: 1.081 ± 0.028
1.126HisThr: 1.126 ± 0.033
1.705HisVal: 1.705 ± 0.038
0.306HisTrp: 0.306 ± 0.016
0.467HisTyr: 0.467 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.935IleAla: 5.935 ± 0.075
0.3IleCys: 0.3 ± 0.015
3.237IleAsp: 3.237 ± 0.051
2.352IleGlu: 2.352 ± 0.051
0.864IlePhe: 0.864 ± 0.027
4.258IleGly: 4.258 ± 0.06
0.681IleHis: 0.681 ± 0.024
1.402IleIle: 1.402 ± 0.036
0.713IleLys: 0.713 ± 0.026
2.898IleLeu: 2.898 ± 0.049
0.623IleMet: 0.623 ± 0.026
0.826IleAsn: 0.826 ± 0.028
2.098IlePro: 2.098 ± 0.043
0.741IleGln: 0.741 ± 0.025
2.539IleArg: 2.539 ± 0.044
1.926IleSer: 1.926 ± 0.042
2.592IleThr: 2.592 ± 0.049
3.623IleVal: 3.623 ± 0.064
0.341IleTrp: 0.341 ± 0.019
0.627IleTyr: 0.627 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.35LysAla: 2.35 ± 0.051
0.112LysCys: 0.112 ± 0.011
1.017LysAsp: 1.017 ± 0.032
0.841LysGlu: 0.841 ± 0.03
0.484LysPhe: 0.484 ± 0.022
1.491LysGly: 1.491 ± 0.043
0.417LysHis: 0.417 ± 0.02
0.862LysIle: 0.862 ± 0.029
0.676LysLys: 0.676 ± 0.038
1.596LysLeu: 1.596 ± 0.048
0.401LysMet: 0.401 ± 0.019
0.474LysAsn: 0.474 ± 0.023
1.139LysPro: 1.139 ± 0.04
0.577LysGln: 0.577 ± 0.024
1.4LysArg: 1.4 ± 0.035
1.084LysSer: 1.084 ± 0.036
1.187LysThr: 1.187 ± 0.041
1.669LysVal: 1.669 ± 0.047
0.264LysTrp: 0.264 ± 0.016
0.448LysTyr: 0.448 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
13.739LeuAla: 13.739 ± 0.115
0.804LeuCys: 0.804 ± 0.028
6.6LeuAsp: 6.6 ± 0.076
4.546LeuGlu: 4.546 ± 0.061
2.428LeuPhe: 2.428 ± 0.05
8.96LeuGly: 8.96 ± 0.099
2.092LeuHis: 2.092 ± 0.04
3.588LeuIle: 3.588 ± 0.06
1.387LeuLys: 1.387 ± 0.045
8.743LeuLeu: 8.743 ± 0.123
1.675LeuMet: 1.675 ± 0.039
1.49LeuAsn: 1.49 ± 0.035
5.397LeuPro: 5.397 ± 0.068
2.272LeuGln: 2.272 ± 0.047
7.701LeuArg: 7.701 ± 0.09
4.997LeuSer: 4.997 ± 0.065
5.775LeuThr: 5.775 ± 0.068
8.192LeuVal: 8.192 ± 0.102
1.087LeuTrp: 1.087 ± 0.033
1.553LeuTyr: 1.553 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.546MetAla: 2.546 ± 0.049
0.158MetCys: 0.158 ± 0.012
1.03MetAsp: 1.03 ± 0.035
0.779MetGlu: 0.779 ± 0.028
0.612MetPhe: 0.612 ± 0.02
1.637MetGly: 1.637 ± 0.041
0.476MetHis: 0.476 ± 0.022
0.919MetIle: 0.919 ± 0.028
0.399MetLys: 0.399 ± 0.019
2.229MetLeu: 2.229 ± 0.039
0.414MetMet: 0.414 ± 0.018
0.496MetAsn: 0.496 ± 0.021
1.346MetPro: 1.346 ± 0.036
0.592MetGln: 0.592 ± 0.024
1.733MetArg: 1.733 ± 0.041
1.449MetSer: 1.449 ± 0.033
1.802MetThr: 1.802 ± 0.037
1.632MetVal: 1.632 ± 0.04
0.249MetTrp: 0.249 ± 0.013
0.374MetTyr: 0.374 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.32AsnAla: 2.32 ± 0.044
0.146AsnCys: 0.146 ± 0.013
0.93AsnAsp: 0.93 ± 0.034
0.784AsnGlu: 0.784 ± 0.028
0.5AsnPhe: 0.5 ± 0.023
1.59AsnGly: 1.59 ± 0.036
0.345AsnHis: 0.345 ± 0.017
0.796AsnIle: 0.796 ± 0.026
0.395AsnLys: 0.395 ± 0.021
1.73AsnLeu: 1.73 ± 0.04
0.348AsnMet: 0.348 ± 0.016
0.407AsnAsn: 0.407 ± 0.02
1.494AsnPro: 1.494 ± 0.035
0.466AsnGln: 0.466 ± 0.022
1.259AsnArg: 1.259 ± 0.035
0.919AsnSer: 0.919 ± 0.032
1.019AsnThr: 1.019 ± 0.028
1.4AsnVal: 1.4 ± 0.034
0.251AsnTrp: 0.251 ± 0.014
0.427AsnTyr: 0.427 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
8.439ProAla: 8.439 ± 0.113
0.318ProCys: 0.318 ± 0.017
4.54ProAsp: 4.54 ± 0.068
3.918ProGlu: 3.918 ± 0.053
1.526ProPhe: 1.526 ± 0.043
6.649ProGly: 6.649 ± 0.08
1.352ProHis: 1.352 ± 0.031
1.709ProIle: 1.709 ± 0.043
0.944ProLys: 0.944 ± 0.033
4.698ProLeu: 4.698 ± 0.069
1.176ProMet: 1.176 ± 0.032
0.832ProAsn: 0.832 ± 0.031
3.165ProPro: 3.165 ± 0.065
1.729ProGln: 1.729 ± 0.038
4.027ProArg: 4.027 ± 0.065
3.022ProSer: 3.022 ± 0.052
2.975ProThr: 2.975 ± 0.062
5.219ProVal: 5.219 ± 0.058
0.81ProTrp: 0.81 ± 0.023
1.103ProTyr: 1.103 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.515GlnAla: 3.515 ± 0.066
0.204GlnCys: 0.204 ± 0.014
1.414GlnAsp: 1.414 ± 0.038
1.221GlnGlu: 1.221 ± 0.035
0.776GlnPhe: 0.776 ± 0.026
2.107GlnGly: 2.107 ± 0.047
0.629GlnHis: 0.629 ± 0.021
1.242GlnIle: 1.242 ± 0.036
0.568GlnLys: 0.568 ± 0.027
2.684GlnLeu: 2.684 ± 0.05
0.639GlnMet: 0.639 ± 0.025
0.544GlnAsn: 0.544 ± 0.022
1.461GlnPro: 1.461 ± 0.034
1.132GlnGln: 1.132 ± 0.038
2.353GlnArg: 2.353 ± 0.042
1.489GlnSer: 1.489 ± 0.035
1.307GlnThr: 1.307 ± 0.037
2.541GlnVal: 2.541 ± 0.051
0.528GlnTrp: 0.528 ± 0.025
0.599GlnTyr: 0.599 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.631ArgAla: 9.631 ± 0.11
0.575ArgCys: 0.575 ± 0.024
4.472ArgAsp: 4.472 ± 0.066
4.193ArgGlu: 4.193 ± 0.07
2.408ArgPhe: 2.408 ± 0.048
6.475ArgGly: 6.475 ± 0.09
1.776ArgHis: 1.776 ± 0.046
3.731ArgIle: 3.731 ± 0.059
1.529ArgLys: 1.529 ± 0.038
7.41ArgLeu: 7.41 ± 0.096
2.114ArgMet: 2.114 ± 0.044
1.479ArgAsn: 1.479 ± 0.04
4.47ArgPro: 4.47 ± 0.07
1.958ArgGln: 1.958 ± 0.041
7.972ArgArg: 7.972 ± 0.112
4.352ArgSer: 4.352 ± 0.067
4.757ArgThr: 4.757 ± 0.07
5.998ArgVal: 5.998 ± 0.074
1.336ArgTrp: 1.336 ± 0.037
1.765ArgTyr: 1.765 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
7.551SerAla: 7.551 ± 0.097
0.344SerCys: 0.344 ± 0.017
3.219SerAsp: 3.219 ± 0.056
2.565SerGlu: 2.565 ± 0.049
1.412SerPhe: 1.412 ± 0.041
6.341SerGly: 6.341 ± 0.077
0.889SerHis: 0.889 ± 0.029
2.065SerIle: 2.065 ± 0.04
0.906SerLys: 0.906 ± 0.034
4.564SerLeu: 4.564 ± 0.079
1.37SerMet: 1.37 ± 0.033
0.925SerAsn: 0.925 ± 0.03
3.084SerPro: 3.084 ± 0.06
1.273SerGln: 1.273 ± 0.034
3.609SerArg: 3.609 ± 0.053
3.009SerSer: 3.009 ± 0.06
3.27SerThr: 3.27 ± 0.055
4.541SerVal: 4.541 ± 0.067
0.8SerTrp: 0.8 ± 0.025
1.048SerTyr: 1.048 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
8.319ThrAla: 8.319 ± 0.097
0.404ThrCys: 0.404 ± 0.021
3.732ThrAsp: 3.732 ± 0.061
2.879ThrGlu: 2.879 ± 0.05
1.585ThrPhe: 1.585 ± 0.038
6.398ThrGly: 6.398 ± 0.081
1.078ThrHis: 1.078 ± 0.029
2.291ThrIle: 2.291 ± 0.043
0.995ThrLys: 0.995 ± 0.032
5.235ThrLeu: 5.235 ± 0.072
1.156ThrMet: 1.156 ± 0.029
0.968ThrAsn: 0.968 ± 0.03
3.694ThrPro: 3.694 ± 0.06
1.264ThrGln: 1.264 ± 0.033
3.826ThrArg: 3.826 ± 0.06
2.835ThrSer: 2.835 ± 0.053
3.539ThrThr: 3.539 ± 0.071
6.131ThrVal: 6.131 ± 0.067
0.772ThrTrp: 0.772 ± 0.027
1.072ThrTyr: 1.072 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
11.306ValAla: 11.306 ± 0.109
0.768ValCys: 0.768 ± 0.025
6.364ValAsp: 6.364 ± 0.081
4.681ValGlu: 4.681 ± 0.075
2.632ValPhe: 2.632 ± 0.049
7.367ValGly: 7.367 ± 0.088
2.037ValHis: 2.037 ± 0.041
3.517ValIle: 3.517 ± 0.067
1.366ValLys: 1.366 ± 0.044
9.054ValLeu: 9.054 ± 0.102
1.526ValMet: 1.526 ± 0.037
1.604ValAsn: 1.604 ± 0.043
5.151ValPro: 5.151 ± 0.072
2.172ValGln: 2.172 ± 0.044
6.866ValArg: 6.866 ± 0.084
4.474ValSer: 4.474 ± 0.063
5.421ValThr: 5.421 ± 0.071
8.482ValVal: 8.482 ± 0.097
1.045ValTrp: 1.045 ± 0.037
1.584ValTyr: 1.584 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.599TrpAla: 1.599 ± 0.044
0.151TrpCys: 0.151 ± 0.01
0.77TrpAsp: 0.77 ± 0.024
0.651TrpGlu: 0.651 ± 0.026
0.472TrpPhe: 0.472 ± 0.021
0.981TrpGly: 0.981 ± 0.032
0.338TrpHis: 0.338 ± 0.017
0.599TrpIle: 0.599 ± 0.026
0.27TrpLys: 0.27 ± 0.017
1.506TrpLeu: 1.506 ± 0.039
0.306TrpMet: 0.306 ± 0.015
0.366TrpAsn: 0.366 ± 0.019
0.731TrpPro: 0.731 ± 0.026
0.524TrpGln: 0.524 ± 0.021
1.189TrpArg: 1.189 ± 0.033
0.948TrpSer: 0.948 ± 0.026
0.853TrpThr: 0.853 ± 0.027
1.014TrpVal: 1.014 ± 0.03
0.339TrpTrp: 0.339 ± 0.015
0.289TrpTyr: 0.289 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.327TyrAla: 2.327 ± 0.048
0.195TyrCys: 0.195 ± 0.014
1.264TyrAsp: 1.264 ± 0.033
1.107TyrGlu: 1.107 ± 0.034
0.733TyrPhe: 0.733 ± 0.024
1.997TyrGly: 1.997 ± 0.039
0.364TyrHis: 0.364 ± 0.019
0.626TyrIle: 0.626 ± 0.022
0.365TyrLys: 0.365 ± 0.02
1.999TyrLeu: 1.999 ± 0.043
0.31TyrMet: 0.31 ± 0.016
0.36TyrAsn: 0.36 ± 0.016
1.144TyrPro: 1.144 ± 0.035
0.554TyrGln: 0.554 ± 0.022
1.685TyrArg: 1.685 ± 0.045
1.162TyrSer: 1.162 ± 0.034
1.104TyrThr: 1.104 ± 0.035
1.573TyrVal: 1.573 ± 0.034
0.335TyrTrp: 0.335 ± 0.017
0.426TyrTyr: 0.426 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3520 proteins (1168249 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski