Roseobacter phage CRP-5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Cobavirinae; Siovirus; unclassified Siovirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QW94|A0A646QW94_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-5 OX=2559284 GN=CRP5_gp05 PE=4 SV=1
MM1 pKa = 7.01CQEE4 pKa = 4.89EE5 pKa = 4.31IDD7 pKa = 3.7MSYY10 pKa = 8.62EE11 pKa = 3.98TNIRR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 5.85DD18 pKa = 3.69GEE20 pKa = 4.26YY21 pKa = 9.96TVYY24 pKa = 11.2GEE26 pKa = 4.42LWDD29 pKa = 5.05DD30 pKa = 5.17GVGFWDD36 pKa = 3.08SWGDD40 pKa = 3.2TGYY43 pKa = 8.83TFEE46 pKa = 4.51VQHH49 pKa = 6.14EE50 pKa = 4.44PEE52 pKa = 4.12FSVTEE57 pKa = 4.54LYY59 pKa = 11.12NKK61 pKa = 10.0DD62 pKa = 3.58GKK64 pKa = 9.64PVPLTTLTPKK74 pKa = 10.31EE75 pKa = 3.87ILVIIDD81 pKa = 5.09IFTQDD86 pKa = 3.21YY87 pKa = 8.65WDD89 pKa = 5.3HH90 pKa = 6.61ILL92 pKa = 4.63

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QW87|A0A646QW87_9CAUD Putative HNH endonuclease OS=Roseobacter phage CRP-5 OX=2559284 GN=CRP5_gp07 PE=4 SV=1
MM1 pKa = 7.52FYY3 pKa = 10.81CIATKK8 pKa = 10.43ALKK11 pKa = 10.67DD12 pKa = 3.43GTNGFRR18 pKa = 11.84FNVCGVKK25 pKa = 10.55GLTRR29 pKa = 11.84KK30 pKa = 9.84RR31 pKa = 11.84LFKK34 pKa = 10.68RR35 pKa = 11.84RR36 pKa = 11.84WGIAKK41 pKa = 8.6GQSMVSLHH49 pKa = 5.94IGLRR53 pKa = 11.84SVYY56 pKa = 9.97IEE58 pKa = 3.92RR59 pKa = 11.84KK60 pKa = 6.72TNKK63 pKa = 9.13TKK65 pKa = 10.69GKK67 pKa = 9.77RR68 pKa = 11.84IRR70 pKa = 11.84HH71 pKa = 4.97FAGG74 pKa = 3.32

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12551

41

1486

216.4

24.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.454 ± 0.485

0.86 ± 0.175

6.597 ± 0.251

6.812 ± 0.289

3.992 ± 0.184

6.804 ± 0.377

1.625 ± 0.214

5.545 ± 0.187

6.581 ± 0.393

7.784 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.176

4.884 ± 0.224

3.307 ± 0.149

3.896 ± 0.222

4.804 ± 0.21

6.852 ± 0.384

7.195 ± 0.32

6.43 ± 0.194

1.53 ± 0.141

3.498 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski