Bacteroidetes bacterium oral taxon 272 str. F0290

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; unclassified Bacteroidetes; Bacteroidetes bacterium oral taxon 272

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S8FIG0|S8FIG0_9BACT Hydrolase carbon-nitrogen family OS=Bacteroidetes bacterium oral taxon 272 str. F0290 OX=888054 GN=HMPREF9012_0070 PE=3 SV=1
MM1 pKa = 7.27AQDD4 pKa = 3.85NDD6 pKa = 3.79FLLEE10 pKa = 4.26DD11 pKa = 4.64AEE13 pKa = 5.63DD14 pKa = 3.8DD15 pKa = 4.05VKK17 pKa = 10.5TIEE20 pKa = 4.26YY21 pKa = 9.96IKK23 pKa = 10.64DD24 pKa = 3.61YY25 pKa = 11.1LPQDD29 pKa = 4.01LKK31 pKa = 11.69NKK33 pKa = 9.99FSEE36 pKa = 3.91EE37 pKa = 3.62DD38 pKa = 2.92LYY40 pKa = 11.7YY41 pKa = 10.1IVDD44 pKa = 4.54VITDD48 pKa = 3.43YY49 pKa = 11.09YY50 pKa = 10.73ANSGILDD57 pKa = 3.64QKK59 pKa = 10.05PDD61 pKa = 3.52KK62 pKa = 10.7DD63 pKa = 3.82GYY65 pKa = 10.43IAIDD69 pKa = 3.21QDD71 pKa = 4.21AIVAYY76 pKa = 9.64ILAAAEE82 pKa = 4.03KK83 pKa = 10.34DD84 pKa = 3.54GMGPYY89 pKa = 9.92EE90 pKa = 4.75ADD92 pKa = 3.08DD93 pKa = 3.57VFFVVQGEE101 pKa = 4.17AEE103 pKa = 4.14YY104 pKa = 11.21AEE106 pKa = 4.5KK107 pKa = 10.87SEE109 pKa = 4.28EE110 pKa = 3.9

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S8GQ68|S8GQ68_9BACT Transcriptional regulator PadR family OS=Bacteroidetes bacterium oral taxon 272 str. F0290 OX=888054 GN=HMPREF9012_1163 PE=4 SV=1
MM1 pKa = 7.71NYY3 pKa = 9.33TYY5 pKa = 11.42YY6 pKa = 11.22SFFPHH11 pKa = 7.57AKK13 pKa = 7.23RR14 pKa = 11.84TPMKK18 pKa = 10.4DD19 pKa = 3.49FLFTRR24 pKa = 11.84HH25 pKa = 5.51YY26 pKa = 8.66RR27 pKa = 11.84TNNKK31 pKa = 9.55KK32 pKa = 9.85KK33 pKa = 9.94EE34 pKa = 3.76KK35 pKa = 8.86TIRR38 pKa = 11.84LIYY41 pKa = 8.67PQWQGGHH48 pKa = 4.22VAALIKK54 pKa = 9.94EE55 pKa = 4.53VKK57 pKa = 9.42DD58 pKa = 3.23TTTASQGYY66 pKa = 7.38SRR68 pKa = 11.84RR69 pKa = 11.84HH70 pKa = 5.49SNVGQSRR77 pKa = 11.84PHH79 pKa = 6.64RR80 pKa = 11.84DD81 pKa = 2.9LEE83 pKa = 4.04GRR85 pKa = 11.84MFGQRR90 pKa = 11.84GALYY94 pKa = 10.64LSRR97 pKa = 11.84QEE99 pKa = 4.0IQVIAIKK106 pKa = 10.27FRR108 pKa = 11.84NTANTFVRR116 pKa = 11.84PMKK119 pKa = 10.0SQRR122 pKa = 11.84LSFIKK127 pKa = 10.51ASLSGPCRR135 pKa = 11.84QDD137 pKa = 2.66VCLLLSVFLTIIDD150 pKa = 3.9NVRR153 pKa = 11.84HH154 pKa = 5.57ILFRR158 pKa = 11.84PHH160 pKa = 6.78DD161 pKa = 3.5QRR163 pKa = 11.84LNVGEE168 pKa = 4.01QGMRR172 pKa = 11.84PCRR175 pKa = 11.84KK176 pKa = 9.1SVFYY180 pKa = 10.1PGRR183 pKa = 11.84HH184 pKa = 4.78LRR186 pKa = 11.84INRR189 pKa = 11.84SGDD192 pKa = 2.98KK193 pKa = 10.71SVFFQYY199 pKa = 9.48PQRR202 pKa = 11.84RR203 pKa = 11.84GKK205 pKa = 10.13HH206 pKa = 4.62FLRR209 pKa = 11.84DD210 pKa = 3.22IRR212 pKa = 11.84NCSSQVAEE220 pKa = 4.11THH222 pKa = 6.37LLCLRR227 pKa = 11.84QCVDD231 pKa = 2.94NQKK234 pKa = 10.51RR235 pKa = 11.84PFIAHH240 pKa = 7.25ASQNVANGTLRR251 pKa = 11.84IVGVQYY257 pKa = 10.65CRR259 pKa = 11.84SIHH262 pKa = 5.65ILCFFRR268 pKa = 11.84ICKK271 pKa = 10.04SNQSINDD278 pKa = 3.86CKK280 pKa = 9.86QKK282 pKa = 10.64KK283 pKa = 8.1YY284 pKa = 9.67EE285 pKa = 3.96KK286 pKa = 10.21KK287 pKa = 9.78YY288 pKa = 9.44RR289 pKa = 11.84CKK291 pKa = 10.81GLIPNNRR298 pKa = 11.84YY299 pKa = 9.98LGVTICVSSAYY310 pKa = 10.16LFCIYY315 pKa = 10.6FSYY318 pKa = 10.53TFAAA322 pKa = 4.46

Molecular weight: 37.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2103

0

2103

746167

37

1954

354.8

40.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.379 ± 0.046

1.197 ± 0.022

5.615 ± 0.035

6.49 ± 0.043

4.569 ± 0.037

6.887 ± 0.045

2.07 ± 0.022

6.815 ± 0.048

6.521 ± 0.048

9.047 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.021

4.718 ± 0.046

3.835 ± 0.029

3.26 ± 0.026

5.379 ± 0.039

5.799 ± 0.041

5.578 ± 0.037

6.475 ± 0.033

1.259 ± 0.02

4.358 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski