Beihai tiger crab virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK03|A0A1L3KK03_9VIRU Uncharacterized protein OS=Beihai tiger crab virus 1 OX=1922711 PE=4 SV=1
MM1 pKa = 6.9NQLRR5 pKa = 11.84SSPSSLVLLAQSLSPNWIICLFLINTNPLIMVNVQQRR42 pKa = 11.84INLGDD47 pKa = 3.65GGVWPLIAAAFLLFDD62 pKa = 3.94TQLSLLITNTNTIISVLEE80 pKa = 3.77ISRR83 pKa = 11.84EE84 pKa = 4.0IQSNTFVTLSDD95 pKa = 3.22IRR97 pKa = 11.84KK98 pKa = 9.42DD99 pKa = 3.28LYY101 pKa = 11.65EE102 pKa = 3.94MMKK105 pKa = 10.24EE106 pKa = 4.22LEE108 pKa = 4.28AQTGEE113 pKa = 4.2LGGIDD118 pKa = 4.48AEE120 pKa = 4.26IFLTNSRR127 pKa = 11.84LADD130 pKa = 3.14IKK132 pKa = 11.08LQMLEE137 pKa = 3.78TGAIIEE143 pKa = 4.69VINEE147 pKa = 3.68GLIGVFEE154 pKa = 4.31TAVKK158 pKa = 10.52NAVSNQEE165 pKa = 3.33TSYY168 pKa = 11.91AMQINNANWEE178 pKa = 4.13IQEE181 pKa = 4.31AATEE185 pKa = 5.08LIQQTLDD192 pKa = 3.81AILVKK197 pKa = 10.44IPAEE201 pKa = 3.92PLLARR206 pKa = 11.84LNMAVTSIEE215 pKa = 4.1YY216 pKa = 10.52ANRR219 pKa = 11.84DD220 pKa = 3.25AVQWDD225 pKa = 3.53GDD227 pKa = 3.78FCEE230 pKa = 4.63NDD232 pKa = 3.2PFGFGPINCEE242 pKa = 4.09GIEE245 pKa = 4.12EE246 pKa = 4.04EE247 pKa = 4.55AGTINGIVFVV257 pKa = 4.39

Molecular weight: 28.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK03|A0A1L3KK03_9VIRU Uncharacterized protein OS=Beihai tiger crab virus 1 OX=1922711 PE=4 SV=1
MM1 pKa = 8.61IMDD4 pKa = 3.95FKK6 pKa = 11.31QRR8 pKa = 11.84FNHH11 pKa = 5.6FRR13 pKa = 11.84QIGLIPDD20 pKa = 3.4RR21 pKa = 11.84DD22 pKa = 3.65VEE24 pKa = 4.49YY25 pKa = 11.0EE26 pKa = 3.85RR27 pKa = 11.84TLNGWKK33 pKa = 10.26ALFVIRR39 pKa = 11.84GRR41 pKa = 11.84MYY43 pKa = 10.53QVTEE47 pKa = 4.11RR48 pKa = 11.84TKK50 pKa = 10.73SEE52 pKa = 4.06AFNEE56 pKa = 4.12LARR59 pKa = 11.84VVVQHH64 pKa = 6.7AEE66 pKa = 3.82SEE68 pKa = 4.63RR69 pKa = 11.84YY70 pKa = 9.49AKK72 pKa = 10.25AAPSLPPPFAHH83 pKa = 6.14LQEE86 pKa = 4.37YY87 pKa = 9.53CQLSPSAPVDD97 pKa = 3.46IPPPAPALLPEE108 pKa = 4.86PKK110 pKa = 9.25MAYY113 pKa = 10.21NPDD116 pKa = 3.09SFIRR120 pKa = 11.84AGGTFTFNTSFSGDD134 pKa = 2.84GFLAVFEE141 pKa = 4.14AHH143 pKa = 6.46FPKK146 pKa = 10.54FLSKK150 pKa = 10.68KK151 pKa = 9.06KK152 pKa = 9.04FVKK155 pKa = 10.1RR156 pKa = 11.84GYY158 pKa = 9.07HH159 pKa = 5.81HH160 pKa = 7.02ASKK163 pKa = 10.64SLEE166 pKa = 4.11HH167 pKa = 6.61LLSEE171 pKa = 5.26CFMEE175 pKa = 5.08LNSSFF180 pKa = 3.67

Molecular weight: 20.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3125

180

2005

625.0

69.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.48 ± 0.213

1.6 ± 0.358

5.6 ± 0.413

6.144 ± 0.883

4.544 ± 1.09

6.24 ± 0.406

2.464 ± 0.46

5.088 ± 1.048

5.44 ± 0.867

8.576 ± 0.981

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.104

4.896 ± 0.622

4.896 ± 0.708

3.968 ± 0.388

5.632 ± 0.521

5.824 ± 0.753

6.368 ± 1.243

7.552 ± 0.793

1.152 ± 0.091

3.328 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski