Rhizoctonia solani dsRNA virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5QCB6|U5QCB6_9VIRU Coat protein OS=Rhizoctonia solani dsRNA virus 2 OX=1411681 PE=4 SV=1
MM1 pKa = 7.29NLYY4 pKa = 10.38NRR6 pKa = 11.84VSALFANWFSSPSNLEE22 pKa = 3.87FVGSYY27 pKa = 9.04HH28 pKa = 6.16HH29 pKa = 6.77QPGTVPPNPSTQEE42 pKa = 3.36AHH44 pKa = 7.15KK45 pKa = 10.6RR46 pKa = 11.84FLHH49 pKa = 6.15NVFKK53 pKa = 10.89QHH55 pKa = 6.36LFTYY59 pKa = 9.56EE60 pKa = 3.47LDD62 pKa = 4.05YY63 pKa = 11.34IEE65 pKa = 4.94NEE67 pKa = 3.91HH68 pKa = 6.46RR69 pKa = 11.84RR70 pKa = 11.84SEE72 pKa = 4.15ATPEE76 pKa = 4.39AIEE79 pKa = 4.26NDD81 pKa = 3.52FFANDD86 pKa = 3.98VEE88 pKa = 4.33PHH90 pKa = 7.16DD91 pKa = 4.15IPFDD95 pKa = 3.5VHH97 pKa = 7.54VEE99 pKa = 3.69IGLQCMTDD107 pKa = 3.13AFRR110 pKa = 11.84PPVPCLPAHH119 pKa = 6.22LNDD122 pKa = 4.24VEE124 pKa = 4.12HH125 pKa = 7.16HH126 pKa = 6.92YY127 pKa = 10.22PFKK130 pKa = 9.92WQVNAEE136 pKa = 4.34PPFSTDD142 pKa = 4.82SYY144 pKa = 11.46FLDD147 pKa = 3.46NRR149 pKa = 11.84KK150 pKa = 9.51LFSDD154 pKa = 4.4YY155 pKa = 11.24YY156 pKa = 10.09DD157 pKa = 3.89TEE159 pKa = 4.26SQTWRR164 pKa = 11.84GYY166 pKa = 10.53VDD168 pKa = 3.3PFEE171 pKa = 4.88ANRR174 pKa = 11.84RR175 pKa = 11.84YY176 pKa = 9.77QHH178 pKa = 6.17TKK180 pKa = 10.52DD181 pKa = 3.82KK182 pKa = 11.29EE183 pKa = 4.21GFLNQTVPAKK193 pKa = 10.36FGFMKK198 pKa = 9.96DD199 pKa = 3.91TIFSWTRR206 pKa = 11.84RR207 pKa = 11.84WHH209 pKa = 6.41HH210 pKa = 6.45IIKK213 pKa = 10.42EE214 pKa = 4.27GFQTATNLSSTAYY227 pKa = 10.47LRR229 pKa = 11.84DD230 pKa = 3.33RR231 pKa = 11.84FIFPMLLHH239 pKa = 6.66TKK241 pKa = 8.25TAIVKK246 pKa = 10.27KK247 pKa = 10.36DD248 pKa = 4.02DD249 pKa = 4.05PDD251 pKa = 3.54KK252 pKa = 11.15MRR254 pKa = 11.84TIWGCSKK261 pKa = 10.15PWIIADD267 pKa = 3.44TMFYY271 pKa = 10.05WEE273 pKa = 4.23YY274 pKa = 10.03IAWIKK279 pKa = 9.49KK280 pKa = 9.4HH281 pKa = 6.16PGVTPMLWGYY291 pKa = 7.8EE292 pKa = 4.05TMTGGWMRR300 pKa = 11.84LNSQLFSSYY309 pKa = 10.46LKK311 pKa = 10.44KK312 pKa = 10.88SYY314 pKa = 8.94VTLDD318 pKa = 2.91WSRR321 pKa = 11.84FDD323 pKa = 2.98KK324 pKa = 10.75RR325 pKa = 11.84AYY327 pKa = 10.09FKK329 pKa = 11.06LILAIMCRR337 pKa = 11.84VRR339 pKa = 11.84TFLDD343 pKa = 3.38FDD345 pKa = 3.59NGYY348 pKa = 9.79LPNVNYY354 pKa = 10.02PDD356 pKa = 4.0TRR358 pKa = 11.84TDD360 pKa = 2.92WSPNKK365 pKa = 9.93AQRR368 pKa = 11.84LEE370 pKa = 4.36RR371 pKa = 11.84LWLWTLEE378 pKa = 4.21CLIKK382 pKa = 10.85SPIVLPDD389 pKa = 2.63GRR391 pKa = 11.84MYY393 pKa = 10.25IRR395 pKa = 11.84HH396 pKa = 5.57YY397 pKa = 11.2AGIPSGLFITQLLDD411 pKa = 2.7SWYY414 pKa = 9.89NYY416 pKa = 9.38TMICTILSSIGLNPKK431 pKa = 9.44HH432 pKa = 6.79CIIKK436 pKa = 10.03VQGDD440 pKa = 3.66DD441 pKa = 3.69SIVRR445 pKa = 11.84LGVLIPPEE453 pKa = 3.67AHH455 pKa = 6.29EE456 pKa = 4.75AFLLALQSKK465 pKa = 10.73ADD467 pKa = 4.16FYY469 pKa = 11.32FKK471 pKa = 10.83AIISVDD477 pKa = 3.18KK478 pKa = 11.47SEE480 pKa = 5.8LGNSLNNRR488 pKa = 11.84EE489 pKa = 4.05VLSYY493 pKa = 10.99RR494 pKa = 11.84NYY496 pKa = 10.48NGLPRR501 pKa = 11.84RR502 pKa = 11.84DD503 pKa = 4.13EE504 pKa = 4.27IKK506 pKa = 10.2MLAQFYY512 pKa = 7.91HH513 pKa = 5.93TKK515 pKa = 10.44ARR517 pKa = 11.84NPTPEE522 pKa = 3.62IAMAQAVGFAYY533 pKa = 10.21ASCGTHH539 pKa = 6.09QRR541 pKa = 11.84VLDD544 pKa = 4.21ALEE547 pKa = 4.42HH548 pKa = 6.38VYY550 pKa = 10.52TDD552 pKa = 4.0YY553 pKa = 11.66KK554 pKa = 10.96DD555 pKa = 4.21AGYY558 pKa = 8.48TPNRR562 pKa = 11.84AGLSLVFGNSPDD574 pKa = 3.27IQLPHH579 pKa = 7.67YY580 pKa = 10.36DD581 pKa = 3.3IDD583 pKa = 3.91HH584 pKa = 6.61FPSIEE589 pKa = 4.03EE590 pKa = 3.62IKK592 pKa = 10.86RR593 pKa = 11.84FLTCNSYY600 pKa = 11.59DD601 pKa = 4.04NSVQMAKK608 pKa = 9.48SWPTSYY614 pKa = 10.68FISEE618 pKa = 3.97PCEE621 pKa = 3.91RR622 pKa = 11.84MM623 pKa = 3.3

Molecular weight: 72.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5QCB6|U5QCB6_9VIRU Coat protein OS=Rhizoctonia solani dsRNA virus 2 OX=1411681 PE=4 SV=1
MM1 pKa = 7.37SAKK4 pKa = 9.18TDD6 pKa = 3.67QVSVSAPDD14 pKa = 3.58AAPGNPQTPTAAAVPTAPSANPEE37 pKa = 4.14PASKK41 pKa = 10.73SSTKK45 pKa = 10.63SKK47 pKa = 10.59GVRR50 pKa = 11.84KK51 pKa = 7.63TLAFSSSSPGIAPLLNSIANVNVSIPQRR79 pKa = 11.84NQTNFFFPDD88 pKa = 3.43ATCFMRR94 pKa = 11.84CVSTCDD100 pKa = 3.28QAMSTTKK107 pKa = 10.51KK108 pKa = 10.45FLDD111 pKa = 4.12GTEE114 pKa = 3.59SWTAFVSQYY123 pKa = 10.23YY124 pKa = 10.82ASLLWHH130 pKa = 6.01LQVFRR135 pKa = 11.84TYY137 pKa = 10.59AKK139 pKa = 10.4SGIPDD144 pKa = 3.28QEE146 pKa = 4.12GQEE149 pKa = 3.94FLDD152 pKa = 4.15FFEE155 pKa = 5.72KK156 pKa = 10.61RR157 pKa = 11.84IDD159 pKa = 3.79LLNIPVPGPWIPFLRR174 pKa = 11.84SPSVVEE180 pKa = 4.05AHH182 pKa = 6.49HH183 pKa = 6.27EE184 pKa = 4.38SYY186 pKa = 11.87GNIAPVLPAIATTPLNSANFFSWPPVVSRR215 pKa = 11.84SIPNPLLPLDD225 pKa = 4.03QLHH228 pKa = 6.78ALAGHH233 pKa = 7.56AMGQNNANAFVAYY246 pKa = 8.28TNIMGMQLIGANDD259 pKa = 3.79FNNVASLLLNPTSHH273 pKa = 6.17SWSSASRR280 pKa = 11.84NRR282 pKa = 11.84TATVAEE288 pKa = 5.09FWHH291 pKa = 6.89DD292 pKa = 3.82HH293 pKa = 6.0ANMLPTRR300 pKa = 11.84LVHH303 pKa = 6.27NQAANQEE310 pKa = 4.03INTYY314 pKa = 9.26FRR316 pKa = 11.84YY317 pKa = 10.07FGFEE321 pKa = 4.3SVDD324 pKa = 3.29GTHH327 pKa = 6.25PHH329 pKa = 6.32GMLEE333 pKa = 4.27TVLNDD338 pKa = 3.1MALYY342 pKa = 10.09CQFFKK347 pKa = 11.17GSAPFSTVDD356 pKa = 3.21IVGLGASIPRR366 pKa = 11.84HH367 pKa = 5.42LPISTTAVRR376 pKa = 11.84AFVYY380 pKa = 7.9PTINRR385 pKa = 11.84LRR387 pKa = 11.84ANRR390 pKa = 11.84HH391 pKa = 4.56AQGHH395 pKa = 4.67RR396 pKa = 11.84HH397 pKa = 5.49FPSDD401 pKa = 3.27LLYY404 pKa = 11.39NMSHH408 pKa = 7.14GDD410 pKa = 3.54LTLDD414 pKa = 3.65TVGEE418 pKa = 4.22QYY420 pKa = 11.86AMSTLVNSTFGSILTQNGHH439 pKa = 5.76DD440 pKa = 4.5QIPLDD445 pKa = 4.34DD446 pKa = 3.95QVEE449 pKa = 4.69GPFWAITTMRR459 pKa = 11.84HH460 pKa = 5.22AKK462 pKa = 9.7GFSPIPSFSTVIPAYY477 pKa = 9.63YY478 pKa = 10.18HH479 pKa = 4.73VTTPVTPKK487 pKa = 10.25TSS489 pKa = 2.93

Molecular weight: 53.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1112

489

623

556.0

63.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.554 ± 1.394

1.259 ± 0.272

5.306 ± 0.749

3.957 ± 0.8

6.025 ± 0.058

4.586 ± 0.324

3.867 ± 0.011

5.306 ± 0.497

3.957 ± 0.926

8.004 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.039

5.665 ± 0.415

7.284 ± 0.678

3.507 ± 0.359

4.676 ± 0.613

7.914 ± 1.046

6.924 ± 0.774

5.306 ± 0.637

2.158 ± 0.448

4.227 ± 0.967
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski