Lactobacillus phage ATCCB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5FF61|A0A4Y5FF61_9CAUD Uncharacterized protein OS=Lactobacillus phage ATCCB OX=2510944 GN=ATCCB_0029 PE=4 SV=1
MM1 pKa = 7.58NEE3 pKa = 3.7TEE5 pKa = 3.89EE6 pKa = 4.06QQRR9 pKa = 11.84CKK11 pKa = 10.48YY12 pKa = 10.0CHH14 pKa = 6.73GDD16 pKa = 3.34SEE18 pKa = 6.43LIMDD22 pKa = 4.97DD23 pKa = 3.7GDD25 pKa = 3.69YY26 pKa = 11.52GGIDD30 pKa = 3.57VYY32 pKa = 11.13ISGNILTIDD41 pKa = 4.21YY42 pKa = 10.8INEE45 pKa = 3.83TSINYY50 pKa = 9.61CPMCGRR56 pKa = 11.84KK57 pKa = 9.01LVV59 pKa = 3.43

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5FF74|A0A4Y5FF74_9CAUD PemK-like protein OS=Lactobacillus phage ATCCB OX=2510944 GN=ATCCB_0032 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 10.1KK4 pKa = 10.49LKK6 pKa = 10.75YY7 pKa = 9.86FGLVLVAAFGIFAFSSQAHH26 pKa = 6.9ASTQPVLDD34 pKa = 3.88LSEE37 pKa = 4.0WQGTISHH44 pKa = 6.03SQAIQLRR51 pKa = 11.84QQNPGVILRR60 pKa = 11.84VQYY63 pKa = 10.78GSQYY67 pKa = 10.28ADD69 pKa = 3.0KK70 pKa = 10.88AFAHH74 pKa = 6.49NAMEE78 pKa = 4.61LNSAKK83 pKa = 10.39EE84 pKa = 3.87PFGVYY89 pKa = 9.57SYY91 pKa = 12.04SMYY94 pKa = 10.59TSASDD99 pKa = 3.62ARR101 pKa = 11.84NEE103 pKa = 4.16AKK105 pKa = 9.68TLYY108 pKa = 10.06NRR110 pKa = 11.84SKK112 pKa = 10.73AYY114 pKa = 10.22NPKK117 pKa = 10.01FYY119 pKa = 11.09ANDD122 pKa = 3.44AEE124 pKa = 4.61QYY126 pKa = 6.63TTTSGSYY133 pKa = 10.04RR134 pKa = 11.84SAVKK138 pKa = 9.9AWADD142 pKa = 3.55EE143 pKa = 4.08MHH145 pKa = 7.14KK146 pKa = 9.87LTNKK150 pKa = 9.84PVVLYY155 pKa = 10.49SGSAFYY161 pKa = 10.46RR162 pKa = 11.84SYY164 pKa = 11.0IGTTSGYY171 pKa = 10.69NGFWEE176 pKa = 4.27ANYY179 pKa = 10.66GSRR182 pKa = 11.84RR183 pKa = 11.84WYY185 pKa = 10.89NSALWQYY192 pKa = 9.71TDD194 pKa = 2.81SHH196 pKa = 7.98YY197 pKa = 11.3SIALNKK203 pKa = 10.44SVDD206 pKa = 3.36ASIVMHH212 pKa = 6.13GNWFNTPSKK221 pKa = 11.09SKK223 pKa = 10.29FVYY226 pKa = 10.04GNLRR230 pKa = 11.84VGTLVRR236 pKa = 11.84VEE238 pKa = 4.46KK239 pKa = 10.45NAKK242 pKa = 9.69FYY244 pKa = 9.4STKK247 pKa = 10.75GKK249 pKa = 10.17LDD251 pKa = 3.57PSIAKK256 pKa = 9.96QNLKK260 pKa = 9.93IKK262 pKa = 9.93QIKK265 pKa = 8.67KK266 pKa = 9.47VNVDD270 pKa = 3.24KK271 pKa = 11.4SNEE274 pKa = 3.71IALVYY279 pKa = 10.19HH280 pKa = 6.92GKK282 pKa = 9.5QVIGWVKK289 pKa = 10.6AQDD292 pKa = 3.33LDD294 pKa = 3.92QYY296 pKa = 9.07YY297 pKa = 9.69TSKK300 pKa = 11.14KK301 pKa = 6.78ITKK304 pKa = 9.24VKK306 pKa = 10.5AKK308 pKa = 8.09QTFYY312 pKa = 11.36SYY314 pKa = 11.08VGKK317 pKa = 10.72KK318 pKa = 9.2KK319 pKa = 10.37VKK321 pKa = 10.29KK322 pKa = 9.63NVKK325 pKa = 10.07GDD327 pKa = 3.51VLKK330 pKa = 11.11VSGYY334 pKa = 10.65SFSKK338 pKa = 9.61TNLPRR343 pKa = 11.84FSYY346 pKa = 10.54GKK348 pKa = 9.0YY349 pKa = 8.42TFTANKK355 pKa = 9.69AFVKK359 pKa = 10.04MISTSSKK366 pKa = 8.99NKK368 pKa = 9.64SVKK371 pKa = 9.95KK372 pKa = 10.06SIRR375 pKa = 11.84KK376 pKa = 9.23IYY378 pKa = 7.89TVKK381 pKa = 10.77PGDD384 pKa = 3.85TLSSIAQQYY393 pKa = 7.01GTTVNSLASKK403 pKa = 11.0NNISNPNIIYY413 pKa = 10.47VGEE416 pKa = 4.08KK417 pKa = 10.89LNII420 pKa = 3.78

Molecular weight: 47.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

23815

56

1859

248.1

28.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.61 ± 0.491

0.869 ± 0.124

6.693 ± 0.339

5.736 ± 0.297

4.212 ± 0.274

5.958 ± 0.432

1.864 ± 0.12

7.819 ± 0.321

9.099 ± 0.344

7.894 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.118

7.89 ± 0.314

2.734 ± 0.171

3.813 ± 0.211

3.069 ± 0.136

7.239 ± 0.225

5.963 ± 0.36

5.581 ± 0.164

1.012 ± 0.089

4.38 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski