Rhodococcus phage ReqiDocB7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4P7A3|D4P7A3_9CAUD Gp097 OS=Rhodococcus phage ReqiDocB7 OX=691966 GN=ReqiDocB7gene097 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.31LAGYY6 pKa = 8.88VAAIGIGASIAGSLFVADD24 pKa = 4.05QLDD27 pKa = 3.68TRR29 pKa = 11.84GFEE32 pKa = 4.32GAEE35 pKa = 3.88LAATTTYY42 pKa = 8.51EE43 pKa = 3.95APIGRR48 pKa = 11.84IEE50 pKa = 4.26EE51 pKa = 5.3DD52 pKa = 3.09SDD54 pKa = 3.94LWDD57 pKa = 4.71CEE59 pKa = 4.22TMGNGICGEE68 pKa = 4.59PIDD71 pKa = 4.36TTVCYY76 pKa = 9.66PKK78 pKa = 10.58YY79 pKa = 10.08FQSLDD84 pKa = 3.24MTFWLNDD91 pKa = 3.41YY92 pKa = 10.05TGGICTDD99 pKa = 3.31AYY101 pKa = 10.16KK102 pKa = 11.02NGVPWPMPGSS112 pKa = 3.49

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4P765|D4P765_9CAUD Tyrosine recombinase/integrase OS=Rhodococcus phage ReqiDocB7 OX=691966 GN=ReqiDocB7gene059 PE=3 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.53KK4 pKa = 10.29SRR6 pKa = 11.84PKK8 pKa = 10.41KK9 pKa = 8.82PTINDD14 pKa = 3.22IPDD17 pKa = 3.4KK18 pKa = 11.01YY19 pKa = 8.51VTCRR23 pKa = 11.84ALMHH27 pKa = 6.2AWKK30 pKa = 9.51YY31 pKa = 8.38HH32 pKa = 3.97TVEE35 pKa = 4.13RR36 pKa = 11.84PRR38 pKa = 11.84GGFIQVLVCMRR49 pKa = 11.84CPTRR53 pKa = 11.84KK54 pKa = 9.29AQHH57 pKa = 6.46ILRR60 pKa = 11.84DD61 pKa = 3.66GSIKK65 pKa = 10.67KK66 pKa = 9.75NVYY69 pKa = 10.17LYY71 pKa = 10.28PRR73 pKa = 11.84DD74 pKa = 3.68YY75 pKa = 10.85LIPGLGFLTAEE86 pKa = 4.06EE87 pKa = 4.28RR88 pKa = 11.84GQLRR92 pKa = 11.84LRR94 pKa = 11.84AMGLLL99 pKa = 3.79

Molecular weight: 11.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

23514

41

3101

223.9

24.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.297 ± 0.862

0.936 ± 0.139

6.124 ± 0.196

6.728 ± 0.32

3.492 ± 0.143

7.744 ± 0.279

2.007 ± 0.217

5.354 ± 0.127

5.639 ± 0.246

7.621 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.125

4.342 ± 0.143

4.763 ± 0.304

3.81 ± 0.148

5.495 ± 0.265

6.205 ± 0.186

6.252 ± 0.26

6.541 ± 0.206

1.646 ± 0.095

3.096 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski