Alistipes sp. CAG:157

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; environmental samples

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5W570|R5W570_9BACT Uncharacterized protein OS=Alistipes sp. CAG:157 OX=1262692 GN=BN505_00842 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.66GFDD5 pKa = 3.6FVEE8 pKa = 4.81TVSGGADD15 pKa = 2.68IKK17 pKa = 11.03FADD20 pKa = 4.34DD21 pKa = 3.34NRR23 pKa = 11.84VIEE26 pKa = 4.55VGQAMYY32 pKa = 10.58AQAASSLLEE41 pKa = 4.0AVGDD45 pKa = 4.22GYY47 pKa = 11.47YY48 pKa = 10.79YY49 pKa = 10.85NGSIDD54 pKa = 3.35VDD56 pKa = 3.36GDD58 pKa = 3.8GFYY61 pKa = 10.21STLTATLIVYY71 pKa = 10.11RR72 pKa = 11.84EE73 pKa = 4.39AEE75 pKa = 4.32TMPEE79 pKa = 4.15GCTVSHH85 pKa = 6.98ISDD88 pKa = 3.94VVPVWWEE95 pKa = 3.64FVTVVPGAGEE105 pKa = 4.11VLNDD109 pKa = 3.45FCFKK113 pKa = 10.53RR114 pKa = 11.84LKK116 pKa = 10.31DD117 pKa = 3.73TVLVMQQ123 pKa = 5.14

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5WGJ6|R5WGJ6_9BACT TGS domain-containing protein OS=Alistipes sp. CAG:157 OX=1262692 GN=BN505_00526 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.36KK6 pKa = 9.96RR7 pKa = 11.84KK8 pKa = 6.44GHH10 pKa = 6.2KK11 pKa = 9.14MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.3NRR25 pKa = 11.84HH26 pKa = 4.95KK27 pKa = 10.66KK28 pKa = 9.83KK29 pKa = 10.46KK30 pKa = 9.85

Molecular weight: 3.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1828

0

1828

606094

29

2279

331.6

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.062

1.2 ± 0.024

5.605 ± 0.042

6.877 ± 0.051

4.228 ± 0.036

8.157 ± 0.051

1.738 ± 0.026

5.938 ± 0.05

4.403 ± 0.049

8.742 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.025

3.732 ± 0.042

4.288 ± 0.032

2.695 ± 0.031

6.481 ± 0.067

5.662 ± 0.044

5.735 ± 0.048

7.522 ± 0.047

1.101 ± 0.021

4.002 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski