Bacteroidales bacterium Barb6XT

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3068 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A180FAM4|A0A180FAM4_9BACT Uncharacterized protein OS=Bacteroidales bacterium Barb6XT OX=1633202 GN=Barb6XT_00527 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.63NEE4 pKa = 3.98AQHH7 pKa = 6.82LIDD10 pKa = 4.57RR11 pKa = 11.84FLSARR16 pKa = 11.84NEE18 pKa = 4.01GKK20 pKa = 9.81EE21 pKa = 3.97AYY23 pKa = 9.97FDD25 pKa = 3.96ADD27 pKa = 4.35EE28 pKa = 5.68IDD30 pKa = 5.41DD31 pKa = 4.4LLEE34 pKa = 4.28GCEE37 pKa = 3.98NKK39 pKa = 10.32EE40 pKa = 4.36DD41 pKa = 3.81FDD43 pKa = 5.14LYY45 pKa = 11.37EE46 pKa = 4.26EE47 pKa = 4.32VLALGLKK54 pKa = 9.1LHH56 pKa = 7.15PEE58 pKa = 4.38NISLQTKK65 pKa = 9.58RR66 pKa = 11.84CWQYY70 pKa = 11.12VVEE73 pKa = 4.48EE74 pKa = 4.47NYY76 pKa = 7.72TTALEE81 pKa = 4.59LIEE84 pKa = 4.7QIGATGDD91 pKa = 3.54QEE93 pKa = 4.21IEE95 pKa = 4.05AVQLEE100 pKa = 5.14CYY102 pKa = 10.62LMLDD106 pKa = 3.52MTDD109 pKa = 3.25EE110 pKa = 4.56AEE112 pKa = 4.27ALSEE116 pKa = 4.18KK117 pKa = 9.97MIAEE121 pKa = 4.3GRR123 pKa = 11.84EE124 pKa = 4.03SMDD127 pKa = 3.76LVFEE131 pKa = 4.52YY132 pKa = 10.19IVPILNDD139 pKa = 3.08KK140 pKa = 10.61EE141 pKa = 4.39MYY143 pKa = 10.41SLALEE148 pKa = 4.23YY149 pKa = 11.05VNRR152 pKa = 11.84GLEE155 pKa = 4.33LYY157 pKa = 9.93PDD159 pKa = 3.69SLVLKK164 pKa = 10.56EE165 pKa = 4.13EE166 pKa = 4.38LCFLMEE172 pKa = 3.99VAEE175 pKa = 4.55DD176 pKa = 3.79LEE178 pKa = 4.72GAIDD182 pKa = 4.26LCNAMIDD189 pKa = 3.83EE190 pKa = 5.04DD191 pKa = 4.66PYY193 pKa = 11.43SYY195 pKa = 10.78EE196 pKa = 3.66YY197 pKa = 10.55WFTLGRR203 pKa = 11.84LYY205 pKa = 11.1SLTEE209 pKa = 4.01EE210 pKa = 4.18FEE212 pKa = 4.21KK213 pKa = 11.1AIDD216 pKa = 3.9AFDD219 pKa = 4.04FAQTCEE225 pKa = 4.19EE226 pKa = 4.64PDD228 pKa = 4.47LEE230 pKa = 4.8LKK232 pKa = 10.28LLKK235 pKa = 10.21AYY237 pKa = 10.56CFFMSDD243 pKa = 3.44KK244 pKa = 11.2YY245 pKa = 11.26EE246 pKa = 4.19EE247 pKa = 4.11ALKK250 pKa = 10.78LYY252 pKa = 10.34TEE254 pKa = 4.28LLKK257 pKa = 11.09DD258 pKa = 3.55EE259 pKa = 5.08EE260 pKa = 4.54KK261 pKa = 11.12NEE263 pKa = 3.99EE264 pKa = 4.33DD265 pKa = 5.43GEE267 pKa = 5.0GDD269 pKa = 3.66DD270 pKa = 5.04YY271 pKa = 11.39ILEE274 pKa = 4.22RR275 pKa = 11.84VRR277 pKa = 11.84PLLAEE282 pKa = 4.56CYY284 pKa = 8.12MKK286 pKa = 11.06LFDD289 pKa = 5.07YY290 pKa = 10.03EE291 pKa = 3.82AAYY294 pKa = 10.48LILHH298 pKa = 6.42EE299 pKa = 5.64LIADD303 pKa = 3.83DD304 pKa = 5.42AEE306 pKa = 4.27AAEE309 pKa = 4.5YY310 pKa = 10.28EE311 pKa = 4.34ADD313 pKa = 4.66DD314 pKa = 5.21ADD316 pKa = 4.29TEE318 pKa = 4.93ADD320 pKa = 4.19DD321 pKa = 4.84TDD323 pKa = 4.17TDD325 pKa = 3.72DD326 pKa = 3.52TDD328 pKa = 3.87AARR331 pKa = 11.84KK332 pKa = 9.93DD333 pKa = 3.69YY334 pKa = 11.22SPYY337 pKa = 9.79IYY339 pKa = 10.9YY340 pKa = 7.49MHH342 pKa = 7.46CCAQTDD348 pKa = 3.98RR349 pKa = 11.84KK350 pKa = 10.24QDD352 pKa = 3.48ALDD355 pKa = 4.04ALEE358 pKa = 4.2TAATVFGGSAHH369 pKa = 6.82ILSLMAYY376 pKa = 9.65AYY378 pKa = 9.66MEE380 pKa = 4.27SAEE383 pKa = 4.29NDD385 pKa = 3.58DD386 pKa = 4.75NLDD389 pKa = 3.78VINKK393 pKa = 9.28LFEE396 pKa = 5.87LIDD399 pKa = 4.32DD400 pKa = 5.06PGDD403 pKa = 4.15DD404 pKa = 5.3LEE406 pKa = 4.44QDD408 pKa = 3.57EE409 pKa = 5.76DD410 pKa = 4.01NPSDD414 pKa = 3.63KK415 pKa = 11.24GDD417 pKa = 3.45FSEE420 pKa = 4.88TVGKK424 pKa = 8.97MLEE427 pKa = 4.2YY428 pKa = 10.32FKK430 pKa = 10.7KK431 pKa = 10.29IYY433 pKa = 9.9AANPDD438 pKa = 3.25QPYY441 pKa = 10.56INLYY445 pKa = 10.09LATAYY450 pKa = 10.62LINDD454 pKa = 3.36DD455 pKa = 3.95MEE457 pKa = 4.9NFEE460 pKa = 4.22KK461 pKa = 10.48HH462 pKa = 5.78YY463 pKa = 10.8NLLSAEE469 pKa = 4.34EE470 pKa = 4.08LDD472 pKa = 5.01SYY474 pKa = 10.26AKK476 pKa = 10.26KK477 pKa = 10.91YY478 pKa = 9.94NAGDD482 pKa = 3.44SRR484 pKa = 11.84ALRR487 pKa = 11.84TVAEE491 pKa = 4.2KK492 pKa = 9.26HH493 pKa = 5.24TGLRR497 pKa = 11.84DD498 pKa = 3.24LAEE501 pKa = 4.7EE502 pKa = 4.16YY503 pKa = 10.61LSKK506 pKa = 10.93PEE508 pKa = 3.94NSNN511 pKa = 3.08

Molecular weight: 58.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A180EWT5|A0A180EWT5_9BACT Hef nuclease OS=Bacteroidales bacterium Barb6XT OX=1633202 GN=Barb6XT_02755 PE=4 SV=1
MM1 pKa = 8.09AEE3 pKa = 4.02RR4 pKa = 11.84QRR6 pKa = 11.84IASALNDD13 pKa = 3.51LSLRR17 pKa = 11.84EE18 pKa = 3.96VNMRR22 pKa = 11.84TGHH25 pKa = 7.03LYY27 pKa = 10.59DD28 pKa = 4.25PACIPVPVFAGNPVGRR44 pKa = 11.84CLLAQFLNRR53 pKa = 11.84HH54 pKa = 4.66SRR56 pKa = 11.84QRR58 pKa = 11.84HH59 pKa = 4.14NRR61 pKa = 11.84PPLVTVGLKK70 pKa = 10.72LEE72 pKa = 4.65IIKK75 pKa = 9.51VLPFAGIIHH84 pKa = 6.55CTQQGASAKK93 pKa = 10.28SPVVYY98 pKa = 9.3LYY100 pKa = 10.7CILPVPVQMQLLHH113 pKa = 6.83RR114 pKa = 11.84IVVKK118 pKa = 10.06YY119 pKa = 9.61PPADD123 pKa = 3.32GFHH126 pKa = 7.31PVRR129 pKa = 11.84QRR131 pKa = 11.84IVMPSDD137 pKa = 3.03QMVEE141 pKa = 3.77HH142 pKa = 6.31TVRR145 pKa = 11.84HH146 pKa = 6.04LFHH149 pKa = 6.76IQRR152 pKa = 11.84HH153 pKa = 4.62LLYY156 pKa = 10.0IRR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 11.84QHH162 pKa = 5.78QKK164 pKa = 9.62VKK166 pKa = 10.32PIVFAVKK173 pKa = 10.13SYY175 pKa = 9.09PVCFEE180 pKa = 4.0RR181 pKa = 11.84QHH183 pKa = 7.23LSALQIFIGSYY194 pKa = 9.16LGCFRR199 pKa = 11.84KK200 pKa = 10.04HH201 pKa = 4.74IHH203 pKa = 5.79LVQYY207 pKa = 8.19TALPVLKK214 pKa = 10.57VLIRR218 pKa = 11.84VFIFPCFLQCVKK230 pKa = 10.32IFQYY234 pKa = 10.41CFRR237 pKa = 11.84FLFIFRR243 pKa = 11.84KK244 pKa = 10.53GNFHH248 pKa = 5.35TVNRR252 pKa = 11.84RR253 pKa = 11.84SS254 pKa = 3.15

Molecular weight: 29.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3068

0

3068

929218

29

2778

302.9

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.293 ± 0.05

1.169 ± 0.022

5.495 ± 0.03

6.345 ± 0.042

4.576 ± 0.033

7.34 ± 0.053

1.908 ± 0.021

6.412 ± 0.045

6.113 ± 0.044

9.047 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.024

4.7 ± 0.04

3.908 ± 0.026

3.179 ± 0.028

5.12 ± 0.036

6.299 ± 0.04

5.991 ± 0.045

6.672 ± 0.042

1.068 ± 0.014

4.083 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski