Microviridae Fen7895_21

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMH8|A0A0G2UMH8_9VIRU DNA pilot protein VP2 OS=Microviridae Fen7895_21 OX=1655660 PE=4 SV=1
MM1 pKa = 7.19SQRR4 pKa = 11.84ILDD7 pKa = 3.99TGNKK11 pKa = 9.63ALVEE15 pKa = 4.1RR16 pKa = 11.84PVVNVPSNHH25 pKa = 5.92FNLSHH30 pKa = 7.11DD31 pKa = 4.03VKK33 pKa = 10.68LSCNIGQLIPCCTMEE48 pKa = 4.72MLPGDD53 pKa = 3.55RR54 pKa = 11.84VNINTEE60 pKa = 3.67ALVRR64 pKa = 11.84FQPMIAPIMHH74 pKa = 7.04RR75 pKa = 11.84LSIRR79 pKa = 11.84SEE81 pKa = 3.68WFFVPNRR88 pKa = 11.84LLWPKK93 pKa = 9.68WEE95 pKa = 4.82DD96 pKa = 3.99FISPPIEE103 pKa = 4.26GVEE106 pKa = 4.17PPAVPLIAEE115 pKa = 4.58TDD117 pKa = 3.21WSVTPNSLGDD127 pKa = 3.87YY128 pKa = 10.51LGLPLGLFDD137 pKa = 3.61EE138 pKa = 4.83TQPGVSALPFSAYY151 pKa = 8.98QRR153 pKa = 11.84IFYY156 pKa = 10.31DD157 pKa = 3.57WYY159 pKa = 10.35RR160 pKa = 11.84DD161 pKa = 3.12EE162 pKa = 6.33DD163 pKa = 4.69FYY165 pKa = 11.23PLQWQYY171 pKa = 11.91AGQLVDD177 pKa = 5.37GNNGDD182 pKa = 4.21NLDD185 pKa = 4.0EE186 pKa = 5.61LDD188 pKa = 4.35VIRR191 pKa = 11.84IRR193 pKa = 11.84NNDD196 pKa = 2.92RR197 pKa = 11.84DD198 pKa = 4.19YY199 pKa = 10.75FTSAKK204 pKa = 9.58PWAQKK209 pKa = 10.55GVVVTIPLLNTTLTLDD225 pKa = 3.31NAGYY229 pKa = 10.8AFSKK233 pKa = 10.82SDD235 pKa = 3.09GSAPNVGGAKK245 pKa = 9.91FDD247 pKa = 3.54GTGFQLEE254 pKa = 4.81DD255 pKa = 3.41VNGDD259 pKa = 3.65QLFLDD264 pKa = 4.0SPYY267 pKa = 11.0AITPEE272 pKa = 4.38DD273 pKa = 3.29ATAAAGTIEE282 pKa = 4.18QLRR285 pKa = 11.84QAVALQKK292 pKa = 10.62FLEE295 pKa = 4.53ADD297 pKa = 3.05ARR299 pKa = 11.84GGTRR303 pKa = 11.84YY304 pKa = 9.83VEE306 pKa = 4.79LLWQHH311 pKa = 5.77FEE313 pKa = 4.5EE314 pKa = 4.67YY315 pKa = 9.95MEE317 pKa = 5.13DD318 pKa = 3.86YY319 pKa = 10.49RR320 pKa = 11.84AQRR323 pKa = 11.84CEE325 pKa = 4.0YY326 pKa = 10.03IGNTVQPITISEE338 pKa = 4.27VLNTTGTDD346 pKa = 3.31AAPQGTMSGHH356 pKa = 6.69GISVARR362 pKa = 11.84HH363 pKa = 5.51ADD365 pKa = 3.0GLYY368 pKa = 10.03YY369 pKa = 9.99HH370 pKa = 7.12AKK372 pKa = 8.55EE373 pKa = 4.82HH374 pKa = 6.1GFLMCIMSVIPVSGYY389 pKa = 8.4YY390 pKa = 9.81QGVPKK395 pKa = 9.85MWSRR399 pKa = 11.84YY400 pKa = 9.38DD401 pKa = 3.5RR402 pKa = 11.84LDD404 pKa = 3.54YY405 pKa = 10.86AWPEE409 pKa = 3.87FAHH412 pKa = 6.93LGEE415 pKa = 3.93QAILNKK421 pKa = 9.77EE422 pKa = 4.0VYY424 pKa = 10.3YY425 pKa = 10.84DD426 pKa = 3.64VASTDD431 pKa = 3.4PVTGNEE437 pKa = 4.44GVWGYY442 pKa = 8.82VPRR445 pKa = 11.84YY446 pKa = 8.54SEE448 pKa = 4.69YY449 pKa = 10.49RR450 pKa = 11.84IQQNRR455 pKa = 11.84VSGDD459 pKa = 2.92FRR461 pKa = 11.84TSLDD465 pKa = 3.3YY466 pKa = 10.5WHH468 pKa = 7.71LARR471 pKa = 11.84KK472 pKa = 8.16FASLPPLAKK481 pKa = 10.19EE482 pKa = 4.35FLQISQGADD491 pKa = 2.9MDD493 pKa = 4.97RR494 pKa = 11.84IFAVIDD500 pKa = 3.32PTVQHH505 pKa = 6.45VLAHH509 pKa = 6.97WFHH512 pKa = 5.8QLKK515 pKa = 10.16FMRR518 pKa = 11.84KK519 pKa = 8.34LPKK522 pKa = 10.29LVVPSII528 pKa = 3.89

Molecular weight: 59.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK59|A0A0G2UK59_9VIRU Major capsid protein VP1 OS=Microviridae Fen7895_21 OX=1655660 PE=3 SV=1
MM1 pKa = 7.73IMLNLFRR8 pKa = 11.84ISLIGSLLLYY18 pKa = 9.65LIRR21 pKa = 11.84YY22 pKa = 7.84FPFGRR27 pKa = 11.84LLIVIRR33 pKa = 11.84VGRR36 pKa = 11.84LCLLLVRR43 pKa = 11.84LTLAICLSIPLISIMLISSISLLLLVLLVRR73 pKa = 11.84ALNLLMMSLRR83 pKa = 11.84YY84 pKa = 9.65VKK86 pKa = 10.28QLLLSIRR93 pKa = 11.84LKK95 pKa = 10.89RR96 pKa = 11.84PLTVLSLKK104 pKa = 10.43KK105 pKa = 10.31RR106 pKa = 11.84LLL108 pKa = 3.79

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1372

108

528

274.4

30.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.184 ± 1.948

1.02 ± 0.369

5.977 ± 0.905

3.644 ± 0.827

3.863 ± 0.826

5.394 ± 0.787

2.332 ± 0.604

5.685 ± 0.76

4.009 ± 0.459

10.204 ± 2.715

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.629

6.122 ± 1.344

5.539 ± 0.714

5.102 ± 0.89

5.685 ± 1.201

5.758 ± 0.467

5.758 ± 1.105

6.778 ± 0.493

1.312 ± 0.544

3.717 ± 0.94
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski