Halomonas heilongjiangensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7TSN3|A0A2N7TSN3_9GAMM Reactive intermediate/imine deaminase OS=Halomonas heilongjiangensis OX=1387883 GN=C1H66_03805 PE=3 SV=1
MM1 pKa = 7.45SGAEE5 pKa = 4.26SFVPTPLMLSDD16 pKa = 3.78SARR19 pKa = 11.84ARR21 pKa = 11.84LTALIEE27 pKa = 4.19EE28 pKa = 4.61EE29 pKa = 4.57GNAEE33 pKa = 3.96LKK35 pKa = 10.78LRR37 pKa = 11.84VYY39 pKa = 9.06VTGGGCSGFQYY50 pKa = 10.96GFDD53 pKa = 3.67FADD56 pKa = 5.57EE57 pKa = 4.1IAEE60 pKa = 4.75DD61 pKa = 3.7DD62 pKa = 3.84TLIPFGDD69 pKa = 3.56VALVVDD75 pKa = 4.48PLSYY79 pKa = 10.58QYY81 pKa = 11.59LVGSTVDD88 pKa = 4.08FEE90 pKa = 4.62EE91 pKa = 5.06GLAGARR97 pKa = 11.84FLVQNPNATTTCGCGASFMVV117 pKa = 4.17

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7TKY1|A0A2N7TKY1_9GAMM LysR family transcriptional regulator OS=Halomonas heilongjiangensis OX=1387883 GN=C1H66_13395 PE=3 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84PKK5 pKa = 10.5RR6 pKa = 11.84PGSRR10 pKa = 11.84PPSPRR15 pKa = 11.84PSRR18 pKa = 11.84PRR20 pKa = 11.84RR21 pKa = 11.84PRR23 pKa = 11.84RR24 pKa = 11.84PSRR27 pKa = 11.84KK28 pKa = 8.27PNRR31 pKa = 11.84PPRR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84HH38 pKa = 5.11SPPAAVAVVLTTTRR52 pKa = 11.84GRR54 pKa = 11.84SAVVSRR60 pKa = 11.84RR61 pKa = 11.84PRR63 pKa = 11.84PGKK66 pKa = 10.23ASRR69 pKa = 11.84DD70 pKa = 3.61NPLPAFPTTTPAQMRR85 pKa = 11.84GRR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 3.22

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4329

0

4329

1413880

23

2902

326.6

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.69 ± 0.051

0.942 ± 0.011

5.675 ± 0.027

6.671 ± 0.043

3.474 ± 0.023

8.562 ± 0.029

2.528 ± 0.019

4.484 ± 0.028

2.241 ± 0.03

11.739 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.018

2.237 ± 0.021

5.167 ± 0.025

3.419 ± 0.022

7.847 ± 0.043

5.004 ± 0.028

4.812 ± 0.025

7.333 ± 0.03

1.502 ± 0.016

2.267 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski