Aiptasia sp. sea anemone associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL37|A0A0K1RL37_9CIRC Putative replication initiation protein (Fragment) OS=Aiptasia sp. sea anemone associated circular virus OX=1692242 PE=4 SV=1
TT1 pKa = 7.12SSSFSSFSHH10 pKa = 7.17DD11 pKa = 2.77AHH13 pKa = 7.62ISIRR17 pKa = 11.84TVVFHH22 pKa = 5.85RR23 pKa = 11.84QWYY26 pKa = 8.52LVVGRR31 pKa = 11.84EE32 pKa = 3.93IGEE35 pKa = 4.05SGTRR39 pKa = 11.84HH40 pKa = 5.1LQGFVIFHH48 pKa = 5.45QVQSRR53 pKa = 11.84AAVSGYY59 pKa = 9.01IARR62 pKa = 11.84AHH64 pKa = 6.58LEE66 pKa = 3.78PARR69 pKa = 11.84ASSVQASDD77 pKa = 3.55YY78 pKa = 10.53CKK80 pKa = 10.48KK81 pKa = 10.85DD82 pKa = 2.95GDD84 pKa = 3.57FDD86 pKa = 4.36EE87 pKa = 6.03YY88 pKa = 11.27GVCPRR93 pKa = 11.84SGQRR97 pKa = 11.84FDD99 pKa = 4.59LQALLKK105 pKa = 10.33WGDD108 pKa = 3.86DD109 pKa = 4.55FIASHH114 pKa = 6.07KK115 pKa = 9.91RR116 pKa = 11.84APTAHH121 pKa = 6.52EE122 pKa = 4.27CAVEE126 pKa = 4.3QPAAYY131 pKa = 9.96LKK133 pKa = 9.77YY134 pKa = 9.81PRR136 pKa = 11.84LISLFQARR144 pKa = 11.84APPPDD149 pKa = 3.86FGRR152 pKa = 11.84EE153 pKa = 4.1GEE155 pKa = 4.07RR156 pKa = 11.84RR157 pKa = 11.84PWQHH161 pKa = 5.71EE162 pKa = 4.17LEE164 pKa = 5.36DD165 pKa = 4.15EE166 pKa = 5.01LEE168 pKa = 4.73GPCEE172 pKa = 3.6NDD174 pKa = 2.79RR175 pKa = 11.84RR176 pKa = 11.84VTFIVDD182 pKa = 3.38QVGGAGKK189 pKa = 8.11TWFQQWFLSKK199 pKa = 10.85NPARR203 pKa = 11.84AQIVSIGKK211 pKa = 9.53RR212 pKa = 11.84DD213 pKa = 3.97DD214 pKa = 3.4VAHH217 pKa = 6.75TIDD220 pKa = 3.33EE221 pKa = 4.43TKK223 pKa = 10.59EE224 pKa = 3.86VFFFAVPRR232 pKa = 11.84GQMEE236 pKa = 4.23FLRR239 pKa = 11.84YY240 pKa = 9.53EE241 pKa = 3.97ILEE244 pKa = 4.07MLKK247 pKa = 10.83DD248 pKa = 3.66RR249 pKa = 11.84MVFSPKK255 pKa = 9.34YY256 pKa = 7.86ASRR259 pKa = 11.84MKK261 pKa = 10.15FLSVVPHH268 pKa = 6.06VIVFSNEE275 pKa = 3.65LPDD278 pKa = 3.85MNKK281 pKa = 9.31MSLDD285 pKa = 3.0RR286 pKa = 11.84YY287 pKa = 9.59YY288 pKa = 10.29IKK290 pKa = 10.53EE291 pKa = 4.13VNN293 pKa = 3.19

Molecular weight: 33.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL37|A0A0K1RL37_9CIRC Putative replication initiation protein (Fragment) OS=Aiptasia sp. sea anemone associated circular virus OX=1692242 PE=4 SV=1
MM1 pKa = 8.02PYY3 pKa = 10.75GPIVAYY9 pKa = 9.26GAKK12 pKa = 9.81RR13 pKa = 11.84ALIGAAMSTRR23 pKa = 11.84AYY25 pKa = 9.66KK26 pKa = 10.39RR27 pKa = 11.84ARR29 pKa = 11.84TAYY32 pKa = 10.0RR33 pKa = 11.84VGSYY37 pKa = 10.44ARR39 pKa = 11.84AYY41 pKa = 10.15GPTAIRR47 pKa = 11.84AGRR50 pKa = 11.84RR51 pKa = 11.84IWKK54 pKa = 9.61AYY56 pKa = 8.85RR57 pKa = 11.84RR58 pKa = 11.84YY59 pKa = 9.68RR60 pKa = 11.84GKK62 pKa = 10.16NRR64 pKa = 11.84EE65 pKa = 3.89QFSRR69 pKa = 11.84TNIGEE74 pKa = 4.27RR75 pKa = 11.84VGSSSTKK82 pKa = 9.58KK83 pKa = 10.18RR84 pKa = 11.84YY85 pKa = 10.27AFTILNATQGDD96 pKa = 4.13TRR98 pKa = 11.84TLTTHH103 pKa = 7.78LINNIPEE110 pKa = 4.16KK111 pKa = 10.79SALNQNNNRR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84QIVNLRR128 pKa = 11.84GVKK131 pKa = 10.1LCLEE135 pKa = 4.61FRR137 pKa = 11.84NQLARR142 pKa = 11.84PLYY145 pKa = 10.82LNVAVLGQKK154 pKa = 7.25TQNQDD159 pKa = 3.11LPPSVLGFFRR169 pKa = 11.84GFDD172 pKa = 3.32QNSRR176 pKa = 11.84SEE178 pKa = 4.41DD179 pKa = 3.25FTTARR184 pKa = 11.84SANEE188 pKa = 3.76LHH190 pKa = 6.8CLPINPDD197 pKa = 2.76KK198 pKa = 10.71FTVLRR203 pKa = 11.84HH204 pKa = 4.72KK205 pKa = 10.44RR206 pKa = 11.84YY207 pKa = 10.0RR208 pKa = 11.84LIPNSDD214 pKa = 3.08AGTVYY219 pKa = 10.63NDD221 pKa = 3.77HH222 pKa = 6.92SGHH225 pKa = 6.88SYY227 pKa = 11.0MNMDD231 pKa = 2.78WWLKK235 pKa = 8.45VKK237 pKa = 10.56RR238 pKa = 11.84QIRR241 pKa = 11.84YY242 pKa = 7.72TDD244 pKa = 3.52GGDD247 pKa = 3.28IDD249 pKa = 5.64SGSLWLCHH257 pKa = 5.86WCDD260 pKa = 3.74EE261 pKa = 4.31IFAGTFAPVQPDD273 pKa = 3.35AYY275 pKa = 9.81AYY277 pKa = 7.28TIRR280 pKa = 11.84SICYY284 pKa = 8.84FKK286 pKa = 10.88EE287 pKa = 4.12PKK289 pKa = 8.57TT290 pKa = 3.73

Molecular weight: 33.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

583

290

293

291.5

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.233 ± 0.285

1.544 ± 0.133

5.317 ± 0.614

4.803 ± 1.505

5.317 ± 1.122

6.346 ± 0.405

2.916 ± 0.624

5.146 ± 0.273

4.974 ± 0.146

6.69 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.715 ± 0.247

4.117 ± 1.793

4.803 ± 0.236

4.46 ± 0.491

9.605 ± 1.052

6.861 ± 0.736

4.803 ± 1.542

6.003 ± 1.374

1.715 ± 0.007

4.631 ± 0.906
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski