Rat coronavirus Parker

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Murine coronavirus; Rat coronavirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GHS1|C6GHS1_9BETC Hemagglutinin-esterase OS=Rat coronavirus Parker OX=502102 GN=HE PE=3 SV=1
MM1 pKa = 6.88AAKK4 pKa = 9.16MAFADD9 pKa = 4.45KK10 pKa = 10.16PNHH13 pKa = 6.87FINFPLAQFSGFMGKK28 pKa = 8.01YY29 pKa = 10.17LKK31 pKa = 10.62LQSQLVEE38 pKa = 4.08MGLDD42 pKa = 3.76CKK44 pKa = 10.5LQKK47 pKa = 10.45APHH50 pKa = 5.8VSITMLDD57 pKa = 3.36IKK59 pKa = 10.77ADD61 pKa = 3.4QYY63 pKa = 11.35KK64 pKa = 9.6QVEE67 pKa = 4.22FAIQEE72 pKa = 4.16ILDD75 pKa = 4.04DD76 pKa = 3.96LAAYY80 pKa = 9.04EE81 pKa = 4.65GYY83 pKa = 9.96IVFDD87 pKa = 3.86NPHH90 pKa = 5.55MLGRR94 pKa = 11.84CLVLDD99 pKa = 3.42VKK101 pKa = 10.92GFEE104 pKa = 4.26EE105 pKa = 4.24LHH107 pKa = 6.8VDD109 pKa = 2.8IVEE112 pKa = 4.28ILRR115 pKa = 11.84KK116 pKa = 8.86MGCTADD122 pKa = 3.85QSRR125 pKa = 11.84VWIPHH130 pKa = 4.93CTVAQFEE137 pKa = 4.42EE138 pKa = 4.53EE139 pKa = 4.22KK140 pKa = 10.44EE141 pKa = 3.96INAMQFYY148 pKa = 10.13YY149 pKa = 10.98KK150 pKa = 10.69LPFYY154 pKa = 10.68LKK156 pKa = 10.34HH157 pKa = 6.41NNILTDD163 pKa = 3.28SRR165 pKa = 11.84LEE167 pKa = 3.86LVKK170 pKa = 10.59IGSSKK175 pKa = 10.27IDD177 pKa = 3.21GFYY180 pKa = 10.77CSEE183 pKa = 3.98LSVWCGEE190 pKa = 4.13RR191 pKa = 11.84LCYY194 pKa = 10.09KK195 pKa = 10.34PPTPKK200 pKa = 10.32FSDD203 pKa = 2.77IFGYY207 pKa = 10.61CCIEE211 pKa = 4.62KK212 pKa = 10.19IRR214 pKa = 11.84GDD216 pKa = 4.11LEE218 pKa = 5.67IGDD221 pKa = 4.82LPQDD225 pKa = 4.71DD226 pKa = 4.12EE227 pKa = 4.54EE228 pKa = 5.09AWAEE232 pKa = 3.96LSYY235 pKa = 10.57HH236 pKa = 4.98YY237 pKa = 10.55QRR239 pKa = 11.84NTYY242 pKa = 8.74FFRR245 pKa = 11.84YY246 pKa = 9.06VHH248 pKa = 7.01DD249 pKa = 3.58NSIYY253 pKa = 9.87FRR255 pKa = 11.84IVCRR259 pKa = 11.84MKK261 pKa = 11.01GCMCC265 pKa = 5.37

Molecular weight: 30.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GHS4|C6GHS4_9BETC Envelope small membrane protein OS=Rat coronavirus Parker OX=502102 GN=E PE=3 SV=1
MM1 pKa = 7.55AALGHH6 pKa = 6.11KK7 pKa = 10.08AKK9 pKa = 10.41LAAVFIGPFIVACMLGISLVYY30 pKa = 10.3LYY32 pKa = 10.3QLQVQIFHH40 pKa = 6.52VNNTIRR46 pKa = 11.84VTGKK50 pKa = 9.14PATVSYY56 pKa = 9.6TLSTPVTPVATTLDD70 pKa = 3.53GTTYY74 pKa = 10.5TLIRR78 pKa = 11.84PTSSYY83 pKa = 9.51TRR85 pKa = 11.84VYY87 pKa = 10.25LGKK90 pKa = 8.56TRR92 pKa = 11.84GFDD95 pKa = 3.03TSTFGPKK102 pKa = 9.26VLNYY106 pKa = 7.82ITSSKK111 pKa = 9.91PHH113 pKa = 6.34LNSGRR118 pKa = 11.84PYY120 pKa = 10.26TFRR123 pKa = 11.84HH124 pKa = 5.03MPKK127 pKa = 10.18YY128 pKa = 10.15MKK130 pKa = 10.78GLVV133 pKa = 3.3

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14810

88

7172

1481.0

165.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.853 ± 0.275

3.768 ± 0.333

5.692 ± 0.424

4.099 ± 0.245

5.213 ± 0.172

6.138 ± 0.47

1.749 ± 0.105

4.321 ± 0.472

5.719 ± 0.453

9.406 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.178

4.875 ± 0.629

3.788 ± 0.437

3.376 ± 0.397

3.653 ± 0.29

7.245 ± 0.506

5.665 ± 0.213

10.095 ± 0.981

1.296 ± 0.079

4.747 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski