Afifella marina DSM 2698

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Afifellaceae; Afifella; Afifella marina

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5NBI9|A0A1G5NBI9_AFIMA Pilus formation protein N terminal region OS=Afifella marina DSM 2698 OX=1120955 GN=SAMN03080610_01704 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84GTKK5 pKa = 9.69ILGRR9 pKa = 11.84AALAAAFLSIAGVAGAADD27 pKa = 4.47LPGQTYY33 pKa = 10.6EE34 pKa = 4.48PMAAPAPVAWSPWQIRR50 pKa = 11.84LRR52 pKa = 11.84ALAVVPHH59 pKa = 7.16DD60 pKa = 4.49SGSVDD65 pKa = 3.94QIPGSDD71 pKa = 3.17LSYY74 pKa = 10.91SNSVTPEE81 pKa = 3.35LDD83 pKa = 2.49ITYY86 pKa = 9.69FFTPNIAAEE95 pKa = 4.86LILGTTYY102 pKa = 11.41AEE104 pKa = 4.35VEE106 pKa = 3.96GDD108 pKa = 3.99GPISGLDD115 pKa = 4.2AGDD118 pKa = 3.48TWILPPTLTLQYY130 pKa = 10.68HH131 pKa = 5.57FTNFGAFKK139 pKa = 9.23PYY141 pKa = 10.25VGAGVNYY148 pKa = 8.09TFFYY152 pKa = 9.41GTEE155 pKa = 4.08NEE157 pKa = 4.47DD158 pKa = 3.49LAAFDD163 pKa = 4.26IDD165 pKa = 3.76NSFGLALQVGADD177 pKa = 3.61YY178 pKa = 10.19MFNEE182 pKa = 4.18HH183 pKa = 6.69WGLNLDD189 pKa = 3.85VKK191 pKa = 10.95KK192 pKa = 10.98LFLSADD198 pKa = 3.42WDD200 pKa = 3.89GSLADD205 pKa = 3.71GTYY208 pKa = 10.83VSGDD212 pKa = 3.31ADD214 pKa = 4.61LDD216 pKa = 3.49PWLIGAGITYY226 pKa = 10.3KK227 pKa = 10.78FF228 pKa = 3.65

Molecular weight: 24.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5NRL6|A0A1G5NRL6_AFIMA Response regulator receiver domain-containing protein OS=Afifella marina DSM 2698 OX=1120955 GN=SAMN03080610_02542 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84PRR4 pKa = 11.84KK5 pKa = 9.39RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84SWFRR12 pKa = 11.84RR13 pKa = 11.84LVLTLLLVLCLPLFLIPIYY32 pKa = 10.64SIVNPPVTTLMLVKK46 pKa = 10.28RR47 pKa = 11.84LGGAPIEE54 pKa = 4.35RR55 pKa = 11.84RR56 pKa = 11.84WVPLDD61 pKa = 4.81DD62 pKa = 4.53ISPNLVNAVVMAEE75 pKa = 3.84DD76 pKa = 3.7AKK78 pKa = 10.5FCRR81 pKa = 11.84HH82 pKa = 6.04HH83 pKa = 7.11GIDD86 pKa = 3.99LGEE89 pKa = 4.74LRR91 pKa = 11.84AAWRR95 pKa = 11.84EE96 pKa = 3.97AQKK99 pKa = 10.8GGEE102 pKa = 4.03MRR104 pKa = 11.84GASTITMQTVKK115 pKa = 10.75NLFLWPSRR123 pKa = 11.84SYY125 pKa = 10.63VRR127 pKa = 11.84KK128 pKa = 9.69AVEE131 pKa = 4.46FPLALYY137 pKa = 10.92ADD139 pKa = 4.75FVLSKK144 pKa = 10.71RR145 pKa = 11.84RR146 pKa = 11.84ILEE149 pKa = 3.65IYY151 pKa = 10.15LNVVEE156 pKa = 4.72WDD158 pKa = 3.13RR159 pKa = 11.84GIYY162 pKa = 9.34GAEE165 pKa = 3.9AAAQHH170 pKa = 5.84YY171 pKa = 7.91FNRR174 pKa = 11.84PAASLTRR181 pKa = 11.84AEE183 pKa = 4.61GARR186 pKa = 11.84LAATLPAPDD195 pKa = 4.84LRR197 pKa = 11.84NPANPGPVTRR207 pKa = 11.84DD208 pKa = 3.14LARR211 pKa = 11.84LFARR215 pKa = 11.84RR216 pKa = 11.84AAAAGPHH223 pKa = 6.27IDD225 pKa = 4.22CLKK228 pKa = 10.5KK229 pKa = 10.7

Molecular weight: 25.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3662

0

3662

1182367

39

2128

322.9

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.482 ± 0.057

0.799 ± 0.012

5.713 ± 0.037

6.619 ± 0.042

3.844 ± 0.029

8.522 ± 0.04

1.995 ± 0.017

5.148 ± 0.026

3.224 ± 0.03

10.242 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.019

2.42 ± 0.02

5.148 ± 0.033

2.87 ± 0.022

7.237 ± 0.039

5.517 ± 0.031

5.1 ± 0.025

7.355 ± 0.036

1.243 ± 0.016

2.151 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski