Microviridae Fen2266_11

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UME2|A0A0G2UME2_9VIRU Uncharacterized protein OS=Microviridae Fen2266_11 OX=1655652 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.21SSDD6 pKa = 3.6PGLSIVAGAIPIVGGVISSLINNSAVDD33 pKa = 3.85ANNAANRR40 pKa = 11.84AFQVQQQQSQNEE52 pKa = 4.23YY53 pKa = 8.84NQAMWQEE60 pKa = 4.01QTDD63 pKa = 4.0YY64 pKa = 11.69NNPQNMMDD72 pKa = 3.61RR73 pKa = 11.84YY74 pKa = 9.45EE75 pKa = 4.25AAGLNPNLIYY85 pKa = 11.05GNATGNVAAQAPMEE99 pKa = 4.36AKK101 pKa = 9.88VDD103 pKa = 4.11YY104 pKa = 10.27VAQPHH109 pKa = 6.51PSVDD113 pKa = 3.38GSAMAAQAVQSANNTKK129 pKa = 10.33LVAAQSNNLQAQTTNTAEE147 pKa = 3.97DD148 pKa = 3.7TVLKK152 pKa = 10.64KK153 pKa = 10.41FVEE156 pKa = 4.47ALTSAEE162 pKa = 3.87ADD164 pKa = 3.44KK165 pKa = 11.07TSAEE169 pKa = 4.03ASFAGPTAQASIGNLLSSSFKK190 pKa = 10.77NQVDD194 pKa = 3.61AQTTSMLAPYY204 pKa = 9.19SANMQAAQTDD214 pKa = 4.0KK215 pKa = 11.43LKK217 pKa = 11.02ADD219 pKa = 3.76TNFTINDD226 pKa = 3.64NVRR229 pKa = 11.84QAALASTTIQEE240 pKa = 4.61AISRR244 pKa = 11.84IAMNAMTNAKK254 pKa = 9.5SAAEE258 pKa = 3.88IQQIRR263 pKa = 11.84ANTQLILGSKK273 pKa = 9.81ALQDD277 pKa = 3.49MDD279 pKa = 4.54LSMRR283 pKa = 11.84NAGMMPHH290 pKa = 7.28DD291 pKa = 4.52GFIQRR296 pKa = 11.84LLGGIGTWITGSNSAGGYY314 pKa = 9.18DD315 pKa = 4.13HH316 pKa = 7.64FDD318 pKa = 3.77GSPSTSSSVSPKK330 pKa = 10.64YY331 pKa = 10.65GDD333 pKa = 4.76PILGNN338 pKa = 3.7

Molecular weight: 35.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK25|A0A0G2UK25_9VIRU DNA pilot protein VP2 OS=Microviridae Fen2266_11 OX=1655652 PE=4 SV=1
MM1 pKa = 7.52ACMFPFFVDD10 pKa = 4.64DD11 pKa = 4.08VPVPCGKK18 pKa = 10.4CPACLVRR25 pKa = 11.84RR26 pKa = 11.84TNNWVFRR33 pKa = 11.84IMQEE37 pKa = 3.77EE38 pKa = 4.27RR39 pKa = 11.84HH40 pKa = 5.96SVSSLWITLTYY51 pKa = 11.0DD52 pKa = 3.34NYY54 pKa = 11.0HH55 pKa = 6.58LPISPNGFATLCKK68 pKa = 9.81RR69 pKa = 11.84DD70 pKa = 3.33VQLFFKK76 pKa = 10.39RR77 pKa = 11.84YY78 pKa = 8.8RR79 pKa = 11.84KK80 pKa = 9.96ALGKK84 pKa = 9.77DD85 pKa = 3.09HH86 pKa = 7.39PKK88 pKa = 9.92IKK90 pKa = 10.6YY91 pKa = 6.96YY92 pKa = 11.09ACGEE96 pKa = 4.24YY97 pKa = 10.43GSKK100 pKa = 10.06GQRR103 pKa = 11.84PHH105 pKa = 5.31YY106 pKa = 9.79HH107 pKa = 6.54LLIINGVDD115 pKa = 2.92RR116 pKa = 11.84CLASSWRR123 pKa = 11.84DD124 pKa = 3.35SEE126 pKa = 4.49GVQIGDD132 pKa = 3.68IYY134 pKa = 10.83IDD136 pKa = 4.04DD137 pKa = 4.36RR138 pKa = 11.84PLNSSAIGYY147 pKa = 6.58TVGYY151 pKa = 8.36MNKK154 pKa = 8.62TKK156 pKa = 9.38ITGRR160 pKa = 11.84YY161 pKa = 7.41QRR163 pKa = 11.84DD164 pKa = 3.42DD165 pKa = 3.6RR166 pKa = 11.84VSEE169 pKa = 3.99FALMSKK175 pKa = 10.61KK176 pKa = 9.79MGLSYY181 pKa = 9.36LTPEE185 pKa = 3.77VVAWHH190 pKa = 6.5KK191 pKa = 11.47ADD193 pKa = 3.75LSRR196 pKa = 11.84NYY198 pKa = 10.14VILPGGVKK206 pKa = 10.04SALPRR211 pKa = 11.84YY212 pKa = 8.58FADD215 pKa = 5.4RR216 pKa = 11.84IYY218 pKa = 11.23SDD220 pKa = 3.47EE221 pKa = 4.41DD222 pKa = 3.31KK223 pKa = 10.52EE224 pKa = 4.22ARR226 pKa = 11.84IAILKK231 pKa = 10.11LLFVKK236 pKa = 10.55LLSLNMMTMFVNLVMLMDD254 pKa = 4.93LLSLKK259 pKa = 8.94MRR261 pKa = 11.84PGMLQLLTFKK271 pKa = 10.34KK272 pKa = 10.45RR273 pKa = 11.84LMAVSTYY280 pKa = 11.2DD281 pKa = 3.75KK282 pKa = 10.92NFCAFGAYY290 pKa = 9.23HH291 pKa = 7.11

Molecular weight: 33.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1373

88

554

274.6

30.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.124 ± 2.017

1.092 ± 0.541

6.555 ± 0.359

4.224 ± 0.649

4.006 ± 0.691

6.118 ± 0.747

2.112 ± 0.486

5.317 ± 0.089

4.807 ± 1.558

8.23 ± 1.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.496 ± 0.59

5.681 ± 1.201

5.171 ± 0.749

5.025 ± 1.357

4.443 ± 1.05

6.773 ± 1.033

5.899 ± 0.972

6.045 ± 0.515

1.311 ± 0.44

3.569 ± 0.757
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski