Mosquito VEM virus SDRBAJ

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F6KIE6|F6KIE6_9VIRU Uncharacterized protein OS=Mosquito VEM virus SDRBAJ OX=1034806 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 3.71MRR4 pKa = 11.84DD5 pKa = 3.61NPRR8 pKa = 11.84SLSMSSTDD16 pKa = 3.38GSPEE20 pKa = 3.35ISSRR24 pKa = 11.84DD25 pKa = 3.59SQIATPSLWRR35 pKa = 11.84PKK37 pKa = 10.59AEE39 pKa = 4.51PFNSWRR45 pKa = 11.84RR46 pKa = 11.84EE47 pKa = 4.07SLSPPTARR55 pKa = 11.84RR56 pKa = 11.84SPGINQDD63 pKa = 2.72WAQWDD68 pKa = 3.94EE69 pKa = 4.3DD70 pKa = 4.61FKK72 pKa = 11.45HH73 pKa = 6.19LWGTYY78 pKa = 9.8SMSGPQSAMKK88 pKa = 10.37QPIANNWMKK97 pKa = 10.87SDD99 pKa = 3.78PEE101 pKa = 4.33VIHH104 pKa = 6.48FLEE107 pKa = 4.7EE108 pKa = 3.68QWEE111 pKa = 3.97QDD113 pKa = 3.22PFEE116 pKa = 4.64VKK118 pKa = 10.36SVTWW122 pKa = 3.44

Molecular weight:
14.2 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F6KIE6|F6KIE6_9VIRU Uncharacterized protein OS=Mosquito VEM virus SDRBAJ OX=1034806 PE=4 SV=1
MM1 pKa = 7.66APGKK5 pKa = 10.29KK6 pKa = 9.45SLHH9 pKa = 5.89SSEE12 pKa = 4.83SNTIRR17 pKa = 11.84EE18 pKa = 4.47HH19 pKa = 5.39YY20 pKa = 7.53QTLVVFGAGGTSLKK34 pKa = 10.8LIGEE38 pKa = 4.82IFRR41 pKa = 11.84SLYY44 pKa = 9.62SQIPIWLRR52 pKa = 11.84GIIIGKK58 pKa = 8.64YY59 pKa = 8.56PISIFLFRR67 pKa = 11.84SRR69 pKa = 11.84FRR71 pKa = 11.84HH72 pKa = 4.32NMIWEE77 pKa = 4.34GLTTGAGASGRR88 pKa = 11.84TGQRR92 pKa = 11.84LSGSRR97 pKa = 11.84EE98 pKa = 3.76GRR100 pKa = 11.84PHH102 pKa = 6.98RR103 pKa = 11.84GCGALLAHH111 pKa = 6.53GPEE114 pKa = 4.59VGGNTYY120 pKa = 9.52PHH122 pKa = 7.25LWSTHH127 pKa = 3.94TSGPRR132 pKa = 11.84ASLWANPSNKK142 pKa = 9.05INPVIHH148 pKa = 5.92YY149 pKa = 9.83VYY151 pKa = 10.24TDD153 pKa = 4.14SINDD157 pKa = 3.1NWALNLAKK165 pKa = 10.24VYY167 pKa = 9.96KK168 pKa = 10.2IPVIHH173 pKa = 7.05KK174 pKa = 8.75PRR176 pKa = 11.84SGPEE180 pKa = 3.5TTYY183 pKa = 10.04WPSGHH188 pKa = 6.52IQTMPRR194 pKa = 11.84YY195 pKa = 8.85SKK197 pKa = 9.84PIPMSINRR205 pKa = 11.84RR206 pKa = 11.84RR207 pKa = 11.84AWQAAKK213 pKa = 10.37DD214 pKa = 3.78KK215 pKa = 10.99RR216 pKa = 11.84YY217 pKa = 9.57IAYY220 pKa = 9.27GKK222 pKa = 10.26ARR224 pKa = 11.84ATVVVPGYY232 pKa = 8.88TRR234 pKa = 11.84TGGTFGRR241 pKa = 11.84FTGGPGSEE249 pKa = 3.75NKK251 pKa = 9.95FFDD254 pKa = 3.56TALGFSFDD262 pKa = 3.76STGEE266 pKa = 4.17VPATGQLNLIPQGVTEE282 pKa = 4.17STRR285 pKa = 11.84VGRR288 pKa = 11.84SCILKK293 pKa = 10.17SIQIKK298 pKa = 10.21GRR300 pKa = 11.84LRR302 pKa = 11.84FDD304 pKa = 3.67PGAAADD310 pKa = 3.98SSTIAYY316 pKa = 9.39LYY318 pKa = 10.43LVQDD322 pKa = 4.02SQCNGAAAAVTDD334 pKa = 4.11VLTSATMAGAIVNMDD349 pKa = 2.83NSQRR353 pKa = 11.84FRR355 pKa = 11.84ILKK358 pKa = 9.02KK359 pKa = 8.56WTWALNSASGVSTAYY374 pKa = 9.34NTVDD378 pKa = 3.82RR379 pKa = 11.84AIDD382 pKa = 3.78YY383 pKa = 9.48YY384 pKa = 10.47RR385 pKa = 11.84KK386 pKa = 9.79CHH388 pKa = 6.23IPLDD392 pKa = 4.27FSSTTGAIGEE402 pKa = 4.12IRR404 pKa = 11.84SNNVFLLAGSADD416 pKa = 3.75TDD418 pKa = 3.81DD419 pKa = 5.19LVAFSGVARR428 pKa = 11.84VRR430 pKa = 11.84FSDD433 pKa = 3.48KK434 pKa = 11.17

Molecular weight:
47.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

725

122

434

241.7

26.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.0 ± 0.885

1.103 ± 0.436

4.414 ± 0.804

4.414 ± 1.585

3.862 ± 0.16

8.414 ± 1.672

2.621 ± 0.339

6.069 ± 0.915

5.103 ± 0.859

6.207 ± 0.593

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.816

4.0 ± 0.369

6.069 ± 1.107

3.034 ± 0.739

6.897 ± 0.412

9.655 ± 1.461

6.483 ± 0.976

4.966 ± 0.678

2.759 ± 1.036

4.0 ± 0.995

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski