Pedobacter sp. RP-1-14

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0ND33|A0A4R0ND33_9SPHI AhpC/TSA family protein OS=Pedobacter sp. RP-1-14 OX=2530453 GN=EZ437_18355 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 6.13DD3 pKa = 3.67SIKK6 pKa = 11.09GLVPHH11 pKa = 6.21VLCFIINEE19 pKa = 4.04LCKK22 pKa = 10.89YY23 pKa = 10.38GFLLAHH29 pKa = 6.61EE30 pKa = 5.13NDD32 pKa = 3.74LADD35 pKa = 4.77LKK37 pKa = 11.4GLVDD41 pKa = 4.86ADD43 pKa = 4.6SISPDD48 pKa = 3.47DD49 pKa = 4.3FEE51 pKa = 5.88LLEE54 pKa = 4.41SVDD57 pKa = 4.29DD58 pKa = 4.09EE59 pKa = 4.89VVQILLNSVEE69 pKa = 4.3KK70 pKa = 11.12VVDD73 pKa = 3.61CSKK76 pKa = 11.34AYY78 pKa = 10.38FLINNLDD85 pKa = 3.55EE86 pKa = 4.47MEE88 pKa = 4.14VMEE91 pKa = 4.3NEE93 pKa = 4.58EE94 pKa = 4.23YY95 pKa = 11.75NMLASDD101 pKa = 4.01NYY103 pKa = 8.77FTYY106 pKa = 10.34IIDD109 pKa = 3.89WDD111 pKa = 3.83NKK113 pKa = 10.51SYY115 pKa = 11.57NDD117 pKa = 4.58LLINLNSVYY126 pKa = 10.35FSISQLIYY134 pKa = 9.29HH135 pKa = 6.05TTCQIRR141 pKa = 11.84LNEE144 pKa = 4.05VEE146 pKa = 5.0VPDD149 pKa = 3.66EE150 pKa = 4.26VYY152 pKa = 11.15EE153 pKa = 4.12EE154 pKa = 4.61FLDD157 pKa = 4.0KK158 pKa = 11.42YY159 pKa = 11.43SDD161 pKa = 3.21ILTEE165 pKa = 5.05KK166 pKa = 10.54IPANDD171 pKa = 3.45KK172 pKa = 10.98NISLLYY178 pKa = 10.51DD179 pKa = 4.52LIVGLNADD187 pKa = 4.37LFKK190 pKa = 10.29IDD192 pKa = 4.53KK193 pKa = 10.3LSNDD197 pKa = 3.38TQTPP201 pKa = 3.39

Molecular weight: 23.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0NKF8|A0A4R0NKF8_9SPHI Glycosyl-4 4'-diaponeurosporenoate acyltransferase OS=Pedobacter sp. RP-1-14 OX=2530453 GN=EZ437_10590 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.3HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.54GRR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84LTVSSEE47 pKa = 3.76GRR49 pKa = 11.84HH50 pKa = 4.97KK51 pKa = 11.04AA52 pKa = 3.25

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4198

0

4198

1552746

9

3099

369.9

41.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.422 ± 0.035

0.731 ± 0.011

5.418 ± 0.023

5.725 ± 0.033

4.875 ± 0.03

6.952 ± 0.036

1.756 ± 0.016

7.334 ± 0.034

7.168 ± 0.036

9.534 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.015

5.649 ± 0.036

3.755 ± 0.02

3.588 ± 0.02

3.961 ± 0.021

6.521 ± 0.032

5.665 ± 0.035

6.36 ± 0.029

1.163 ± 0.016

4.082 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski