Lactococcus phage P4565

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus P4565

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V487|A0A649V487_9CAUD Uncharacterized protein OS=Lactococcus phage P4565 OX=2662297 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.41VYY4 pKa = 10.54VLSGDD9 pKa = 4.17TYY11 pKa = 9.3CGSWGSEE18 pKa = 3.01ISLFGVFSNKK28 pKa = 9.73EE29 pKa = 4.0EE30 pKa = 4.09ADD32 pKa = 3.56KK33 pKa = 11.28LADD36 pKa = 4.3EE37 pKa = 4.52MQCDD41 pKa = 3.37ISVVNIDD48 pKa = 3.63EE49 pKa = 4.27VEE51 pKa = 4.03EE52 pKa = 4.09PKK54 pKa = 10.79RR55 pKa = 11.84LGGYY59 pKa = 9.95CEE61 pKa = 3.92

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V335|A0A649V335_9CAUD Uncharacterized protein OS=Lactococcus phage P4565 OX=2662297 PE=4 SV=1
MM1 pKa = 7.71AMNSLNTSIIAKK13 pKa = 9.7EE14 pKa = 3.96MQTKK18 pKa = 7.47VTEE21 pKa = 4.24RR22 pKa = 11.84MGDD25 pKa = 2.9WFEE28 pKa = 5.71AEE30 pKa = 4.89FKK32 pKa = 11.05AKK34 pKa = 10.55ANAAARR40 pKa = 11.84RR41 pKa = 11.84TRR43 pKa = 11.84LIRR46 pKa = 11.84SHH48 pKa = 5.25GHH50 pKa = 5.21TYY52 pKa = 9.84TYY54 pKa = 11.14ARR56 pKa = 11.84HH57 pKa = 5.57QNTGQLARR65 pKa = 11.84NLKK68 pKa = 8.49QVKK71 pKa = 10.03KK72 pKa = 9.94GDD74 pKa = 3.72KK75 pKa = 9.93VVVNAGTRR83 pKa = 11.84ANYY86 pKa = 9.92SSGYY90 pKa = 8.88HH91 pKa = 5.38GMYY94 pKa = 10.52FLVEE98 pKa = 4.16KK99 pKa = 10.74KK100 pKa = 10.71GMQDD104 pKa = 3.28VKK106 pKa = 9.28TTLKK110 pKa = 10.68KK111 pKa = 10.0GANYY115 pKa = 9.69ANSMKK120 pKa = 10.65LL121 pKa = 3.26

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

9573

33

934

174.1

19.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.714 ± 0.484

0.606 ± 0.109

5.818 ± 0.211

7.333 ± 0.565

4.293 ± 0.241

6.247 ± 0.465

1.16 ± 0.155

7.26 ± 0.398

9.172 ± 0.448

8.754 ± 0.379

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.196

6.968 ± 0.423

2.235 ± 0.216

3.698 ± 0.266

3.353 ± 0.256

6.383 ± 0.437

6.55 ± 0.337

6.268 ± 0.318

1.4 ± 0.127

4.356 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski